BACKGROUND AND OBJECTIVE: To evaluate the ability of optical coherence tomography (OCT) (a noncontact, high-resolution imaging technique that generates detailed cross-sectional images of tissue structure) to image and document acute response to trauma in the larynx.
MATERIALS AND METHODS: Porcine larynges were dissected to expose the vocal cords. Vocal cords were imaged with 1310 nanometer (nm) light source with a bandwidth of 75 nm at 1 frame/second (s). Vocal cord injuries were simulated by applying topical phenolic acid, injecting titanium dioxide into the submucosa, and carbon dioxide laser irradiation.
RESULTS: The epithelium, basement membrane, and lamina propria were clearly delineated, and the effect of each intervention could be monitored as lesions progressed. The OCT image corresponded closely with histology.
CONCLUSION: OCT is a powerful imaging tool with the potential for use in real time and has potential for multiple clinical applications in the larynx.
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References
1.
HuangDSwansonEALinCP, et al. Optical coherence tomography. Science1991;254:1178–81.
2.
TearneyGJBrezinskiMEBoumaBE, et al. In vivo endoscopic optical biopsy with optical coherence tomography. Science1997;276:2037–9.
3.
FujimotoJGBrezinskiMETearneyGJ, et al. Optical biopsy and imaging using optical coherence tomography. Nat Med1995;1:970–2.
4.
JaffeGJCaprioliJ.Optical coherence tomography to detect and manage retinal disease and glaucoma. Am J Ophthalmol2004;137:156–69.
5.
StangaPEBirdACOptical coherence tomography (OCT): Principles of operation, technology, indications in vitreoretinal imaging and interpretation of results. Int Ophthalmol2001;23:191–7.
6.
BibasAGPodoleanuAGCucuRG, et al. 3-D optical coherence tomography of the laryngeal mucosa. Clin Otolaryngol2004;29:713–20.
7.
BrandSPonerosJMBoumaBE, et al. Optical coherence tomography in the gastrointestinal tract. Endoscopy2000;32:796–803.
8.
BrezinskiM.Characterizing arterial plaque with optical coherence tomography. Curr Opin Cardiol2002;17:648–55.
9.
ColstonBWJr.EverettMJSathyamUS, et al. Imaging of the oral cavity using optical coherence tomography. Monogr Oral Sci2000;17:32–55.
10.
JackleSGladkovaNFeldchteinF, et al. In vivo endoscopic optical coherence tomography of the human gastrointestinal tract-toward optical biopsy. Endoscopy2000;32:743–9.
11.
PanYLiZXieT, et al. Hand-held arthroscopic optical coherence tomography for in vivo high-resolution imaging of articular cartilage. J Biomed Opt2003;8:648–54.
12.
PierceMCStrasswimmerJParkBH, et al. Advances in optical coherence tomography imaging for dermatology. J Invest Dermatol2004;123:458–63.
13.
ShenBJr.ZuccaroG, Optical coherence tomography in the gastrointestinal tract. Gastrointest Endosc Clin N Am2004:14:555–71.
14.
WelzelJ.Optical coherence tomography in dermatology: A review. Skin Res Technol2001;7:1–9.
15.
NassifNAPierceMCParkBH, et al. Quantitative evaluation of osteoarthritis progression using polarization-sensitive optical coherence tomography. Advanced Biomedical and Clinical Diagnostic Systems II; 2004:170–5.
16.
WongBJZhaoYYamaguchiM, et al. Imaging the internal structure of the rat cochlea using optical coherence tomography at 0.827 microm and 1.3 microm. Otolaryngol Head Neck Surg2004;130:334–8.
17.
ShakhovAVTerentjevaABKamenskyVA, et al. Optical coherence tomography monitoring for laser surgery of laryngeal carcinoma. J Surg Oncol2001;77:253–8.
18.
SaxerCEde BoerJFParkBH, et al. High-speed fiber-based polarization-sensitive optical coherence tomography of in vivo human skin. Opt Lett2000;25:1355–7.
19.
ZhaoYChenZSaxerC, et al. Phase-resolved optical coherence tomography and optical Doppler tomography for imaging blood flow in human skin with fast scanning speed and high velocity sensitivity. Opt Lett2000;25:114–6.
20.
JohnsonRESigmanJDFunkGF, et al. Quantification of surgical margin shrinkage in the oral cavity. Head Neck1997;19:281–6.
