Measurements of red cell volume, plasma volume, and tissue haematocrit (Hct) were made in 14 brain regions in adult rats using 51Cr-tagged red cells and 125I-labeled human serum albumin. The mean large vessel (systemic artery) Hct was 41.8, total body Hct was 35.3, and of the brain regions, the lowest value (septal nucleus) was 25.91 and the highest (visual cortex) was 32.05. The lowest blood volume was 6.29 μl g−1 (caudate putamen) and the highest was 14.44 μl g−1 (inferior colliculus). There was a significant difference between regions in both blood volume and tissue blood Hct. When brain regions were ranked in order of blood volume, this did not coincide with the order for blood flow.
Albe-FessardDStutinskyFLiboubanS, (1966) Atlas Sterotaxique du Diencephale du Rat Blanc. Paris, Editions du Centre National de la Recherche Scientifique
2.
EverettNBSimmonsBLasherEP, (1956) Distribution of blood (Fe59) and plasma (I131) volumes of rats determined by liquid nitrogen freezing. Circ Res4:419–424
3.
FenstermacherJDBlasbergRGPatlakCS, (1981) Methods for quantifying the transport of drugs across brain barrier systems. Pharmacol Ther14:217–248
4.
GjeddeA, (1980) Rapid steady-state analysis of blood-brain glucose transfer in rat. Acta Physiol Scand108:331–339
5.
GjeddeAHansenAJSiemkowiczE, (1980) Rapid simultaneous determination of regional blood flow and blood-brain glucose transfer in brain of rat. Acta Physiol Scand108:321–330
6.
HeathDF, (1973) The effect of scald injury upon the distribution of glucose between red cells and plasma and upon the turnover of glucose in red cells in the rat. Br J Exp Pathol54:359–367
7.
HeiseySR, (1968) Brain and choroid plexus blood volumes in invertebrates. Comp Biochem Physiol26:489–498
8.
HugginsRASmithELDeaversS, (1966) Cr51-tagged red cell equilibration in dogs. Am J Physiol211:283–287
9.
IrwinGHPreskornSH, (1982) A dual label radiotracer technique for the simultaneous measurement of cerebral blood flow and the single-transit extraction of diffusion-limited compounds in rats. Brain Res249:23–30
10.
LevinVAAusmanJI, (1969) Relationship of peripheral venous hematocrit to brain hematocrit. J Appl Physiol26:433–437
11.
OhnoKPettigrewKDRapoportSI, (1979) Local cerebral blood flow in the conscious rat as measured with 14C-antipyrine, 14C-iodoantipyrine and 3H-nicotine. Stroke10:62–67
12.
PollayMStevensA, (1979) Simultaneous measurement of regional blood flow and glucose extraction in rat brain. Neurochem Res4:109–123
13.
RapoportSIOhnoKPettigrewKD, (1979) Drug entry into the brain. Brain Res172:354–359
14.
SageJIVan UitertRLDuffyTE, (1981) Simultaneous measurement of cerebral blood flow and unidirectional movement of substances across the blood-brain barrier: Theory, method, and application to leucine. J Neurochem36:1731–1738
