Cortical Substrate Oxidation during Hyperketonemia in the Fasted Anesthetized Rat in Vivo

Abstract

Ketone bodies are important alternate brain fuels, but their capacity to replace glucose and support neural function is unclear. In this study, the contributions of ketone bodies and glucose to cerebral cortical metabolism were measured in vivo in halothane-anesthetized rats fasted for 36 hours (n=6) and receiving intravenous [2,4-¹³C₂]-d-β-hydroxybutyrate (BHB). Time courses of ¹³C-enriched brain amino acids (glutamate-C4, glutamine-C4, and glutamate and glutamine-C3) were measured at 9.4 Tesla using spatially localized ¹H-[¹³C]-nuclear magnetic resonance spectroscopy. Metabolic rates were estimated by fitting a constrained, two-compartment (neuron–astrocyte) metabolic model to the ¹³C time-course data. We found that ketone body oxidation was substantial, accounting for 40% of total substrate oxidation (glucose plus ketone bodies) by neurons and astrocytes. d-β-Hydroxybutyrate was oxidized to a greater extent in neurons than in astrocytes (∼70:30), and followed a pattern closely similar to the metabolism of [1-¹³C]glucose reported in previous studies. Total neuronal tricarboxylic acid cycle (TCA) flux in hyperketonemic rats was similar to values reported for normal (nonketotic) anesthetized rats infused with [1-¹³C]glucose, but neuronal glucose oxidation was 40% to 50% lower, indicating that ketone bodies had compensated for the reduction in glucose use.

Keywords

energy metabolism kinetic modeling MR metabolite MR spectroscopy neuronal–glial interaction

Introduction

d-β-Hydroxylbutyrate (BHB), an end product of liver fatty acid catabolism, is actively used by the brain for energy metabolism, as well as for amino-acid and lipid biosynthesis. Under fasted conditions with declining blood glucose levels, ketone body levels increase from low levels of ∼0.1 to 3 mmol/L. During prolonged fasting or starvation, the brain's utilization of ketone bodies increase, primarily because of an increase in transport from the blood through monocarboxylic transporter proteins (Gjedde and Crone, 1975; Hasselbalch et al, 1996). Reports that ketone bodies are neuroprotective, including the well-known, but poorly understood, anticonvulsant effect of the ketogenic diet suggest that ketone bodies have actions beyond those of a simple fuel or synthetic substrate.

Although brain ketone oxidation can be substantial, the quantitative contribution of ketone bodies to neuronal and astroglial oxidative metabolism, and their capacity to replace glucose in support of neural function, remains unclear. In a study using [U-¹³C]BHB, Kunnecke et al (1993) reported greater oxidation of ketone bodies in astrocytes than in neurons. Increased astroglial acetate metabolism has been observed in mice maintained on a ketogenic diet possibly reflecting adaptation to prolonged ketonemia (Melo et al, 2006; Yudkoff et al, 2005). In contrast, a study of acute hyperketonemia in overnight-fasted humans (Pan et al, 2001, 2002) infused intravenously with [2,4-¹³C₂]-d-BHB revealed a pattern of brain glutamate and glutamine labeling similar to that from [1-¹³C]glucose, indicating that oxidation of BHB, like glucose, occurred mostly in neurons—conclusions in line with results of early ¹⁴C-labeled BHB tracer studies (Hawkins et al, 1986).

Although brain utilization of ketone bodies is increased by fasting, contradictory findings have appeared on the effects of ketone bodies on brain glucose consumption. Early studies using [¹⁴C] 2-deoxyglucose (2-DG) autoradiography showed no effects on glucose uptake and oxygen consumption during acute infusion of BHB or after 3 days of fasting (Corddry et al, 1982), whereas later studies reported that ketone bodies administered acutely or increased over time by the ketogenic diet inhibited brain glucose utilization (LaManna et al, 2009; Melo et al, 2006).

In this study, we investigated the effects of hyperketonemia on cortical glucose and ketone metabolism of anesthetized rats subjected to a prolonged (36 hours) fast. Using [2,4-¹³C₂]-d-BHB as a metabolic substrate administered by intravenous infusion, the turnover of ¹³C-enriched glutamate and glutamine was measured in vivo using ¹H-nuclear magnetic resonance with indirect ¹³C detection (¹H-[¹³C] NMR). The ¹³C-enrichment curves were fitted with a two-compartment metabolic model yielding the rates of BHB and glucose oxidation in neurons and astrocytes, and glutamate/glutamine neurotransmitter cycling.

Materials and methods

Animal Preparation

Experiments were conducted using male Sprague–Dawley rats (weighing 185±15 g) fasted for 36 hours before study (n=6). Rats were anesthetized with halothane (2% induction), tracheotomized, and ventilated (30% oxygen; ∼68% nitrous oxide). After surgery, halothane was reduced to ∼1% and d-tubocurarine-Cl was administered for immobilization. The left femoral artery and vein were catheterized for continuous monitoring of arterial blood pressure and blood sampling, and for infusion of [2.4-¹³C₂]-d-BHB, respectively (Cambridge Isotopes, Andover, MA, USA). Blood gases (pCO₂, pO₂, pH) were measured periodically in arterial blood samples (ABL5 blood gas system, Radiometer America Inc., Westlake, OH, USA). Animal core body temperature was maintained near ∼37°C using a heating pad connected to a temperature-regulated water bath.

Rats received [2,4-¹³C₂]-d-BHB (1.5 mol/L) according to a three-step infusion: 0 to 5 minutes at 600 μmol/min per kg, 5 to 10 minutes at 300 μmol/min per kg, and thereafter at 120 μmol/min per kg. The three-step infusion protocol achieved a relatively rapid increase in plasma BHB with stable maintenance of blood BHB level and ¹³C enrichment over the course of the ∼90-minute infusion.

A separate small group (n=3) of 36 hour-fasted rats was infused with [2-¹³C]acetate (instead of ¹³C-BHB) for 2 hours using a three-step infusion protocol (Jiang et al, 2009) and the brain tissue was studied ex vivo. Cerebral cortical extracts were prepared and ¹³C metabolite enrichments determined to calculate the steady-state value of the flux ratio, V_cyc/V_tcaN, as described previously (Lebon et al, 2002; Patel et al, 2005), and subsequently used to constrain the fits of the metabolic model to the ¹³C-enrichment time courses.

At the end of each animal experiment, the head and brain of the anesthetized animal were frozen in situ with liquid N₂ with continued mechanical ventilation.

All animal experiments were conducted under protocols approved by the Yale Animal Care and Use Committee.

In Vivo Nuclear Magnetic Resonance Spectroscopy

In vivo¹H-[¹³C] NMR spectra were obtained at 9.4 Tesla (Bruker ADVANCE NMR spectrometer/imager, Bruker BioSpin Corp., Billerica, MA, USA) (400.5 MHz for ¹H and 100.71 MHz for ¹³C). The ¹³C-edited ¹H NMR spectra were acquired using a circular surface coil of 14 mm diameter for ¹H detection, in which an outer butterfly coil was used for ¹³C decoupling. Magnetic-field homogeneity was optimized using FASTMAP (Gruetter, 1993). The ¹H-[¹³C] NMR spectra were obtained from a localized volume (6 × 6 × 6 mm³) using a polarization transfer pulse sequence in which the 90° and 180° pulses were replaced with adiabatic half- and full-passage pulses, respectively. Suppression of signals outside the localized volume was achieved with localization by adiabatic selective refocusing (de Graaf, 2007). The ¹H-[¹³C] spectra were acquired with a 2.5-second repetition time in blocks of 512 scans with alternate on/off (180°) inversion of ¹³C. Free induction decays were saved independently and processed in Matlab7.0 (MathWorks, Natick, MA, USA) for analyzing time-dependent changes. The time-dependent labeling of metabolites was analyzed by fitting the ¹H-[¹³C] total and difference spectrum using an in-house written version of the linear-combination model algorithm implemented in Matlab (MathWorks). The concentrations of the metabolites were also determined in the brain extracts at the end point of the labeled isotope infusions.

Preparation of Blood Plasma and Brain Extracts and Nuclear Magnetic Resonance Spectroscopy

Plasma samples were prepared for NMR spectral analysis by the addition of 200 μL of 2.5 mmol/L formic acid and 0.25 mmol/L TSP (3-trimethylsilyl tetradeuterated sodium propionate) and 400 μL 100 mmol/L phosphate buffer (pH 7) to 50 μL blood plasma. The solution was passed through a microcentrifuge filter tube (10,000 Da cutoff, Nanosep Centrifugal Devices, VWR, Batavia, IL, USA). Concentrations and ¹³C enrichments of BHB and other substances (such as acetoacetate, acetone, acetate, lactate) were determined using ¹H-[¹³C] NMR spectroscopy with 20-second repetition time to achieve full spin-lattice T₁ relaxation of the measured metabolite resonances. Plasma and tissue extract samples were measured at 500 MHz (Bruker AVANCE high-resolution NMR spectrometer, Bruker BioSpin Corp., Billerica, MA, USA). Both TSP and formic acid were used as chemical shift and concentration references, respectively.

Ethanol extracts were prepared from the frozen frontoparietal cortex (100 to 150 mg wet weight) using the procedure described in the study by Patel et al (2001). [2-¹³C]glycine (50 μL, 5 mmol/L) was added as an internal concentration reference at the beginning of tissue extraction. After centrifugation and lyophilization of the supernatant, the extract powder was suspended in 600 μL of a buffer solution containing 50 mmol/L phosphate (pH 7), 0.8 mmol/L formic acid, and 0.08 mmol/L TSP in D₂O/H₂O (2:1). The concentrations and ¹³C enrichments of metabolite resonances in the extracted brain samples were measured fully relaxed (repetition time=20 seconds) with ¹H-[¹³C] NMR spectroscopy.

Description of the Metabolic Model Incorporating d-β-Hydroxybutyrate Oxidation

The time courses of brain amino-acid ¹³C enrichments were fitted to a two-compartment metabolic model (Figure 1), using as input the plasma time courses of ¹³C-enriched BHB, glucose, and lactate. In addition, nonlabeled sources (set to natural abundance) were included in the model to represent dilution flows. The metabolic model was fitted to the time courses of Glu-C4, Gln-C4, and Glx-C3 enrichment, along with the end-point enrichments for Gln-C3 and Glu-C3 (determined in the extracts). The differential equations describing the model (mass and isotope balance) were generated using CWave software (GF Mason, New Haven, CT, USA; Mason et al, 2003) and are given in Table 1. The differential equations were solved in Matlab using a first/second-order Runge–Kutta algorithm, and data were fitted using the Levenburg–Marquardt algorithm. Tissue glutamine was assumed to be present entirely in astrocytes, whereas glutamate was divided between neurons (90%) and astrocytes (10%) (Patel et al, 2005; Storm-Mathisen et al, 1983). Pyruvate carboxylase flux (V_pc) was assumed to be 20% of the rate of glutamine synthesis (V_gln) (Sibson et al, 2001). To improve the reliability of flux estimates, the fits were constrained by the value of the ratio, V_cyc/V_tcaN (Patel et al, 2005). The flux ratio was calculated from steady-state Glu-C4 and Gln-C4 ¹³C enrichments (fe_ss) measured in a separate group of 36 hour-fasted animals receiving an infusion of [2-¹³C]acetate according to:

V_{c y c} / V_{t c a N} = (f e_{s s} G l u - C 4_{N} - c) / (f e_{s s} G l n - C 4 - f e_{s s} G l u - C 4_{N})

where ‘c’ is a correction to remove label incorporation arising from ¹³C-labeled plasma glucose (C1,6), lactate (C3), and BHB (C2,4) by peripheral metabolism of the [2-¹³C]acetate. The contribution of ¹³C-labeled plasma glucose and/or lactate to Glu-C4 was determined from the steady-state enrichment of brain lactate C3 (fe_ssLac-C3), which was assumed to be equivalent to the enrichment of pyruvate-C3, multiplied by the fraction of acetyl-CoA ¹³C labeling derived through pyruvate and pyruvate dehydrogenase and given by

f e_{s s} G l u - C 4_{p d h} = f e_{s s} L a c - C 3_{A c} \times (V_{p d h N} / V_{t c a N})

Figure 1

Two-compartment (neuron and astrocyte) model used to illustrate the metabolic pathway of label flow from BHB. CMR_kb=(V_AcCoA−kbA+V_AcCoA−kbN)/2, CMR_glc(ox)=(V_pdhA+V_pdhN+V_pc)/2. The OAA label distribution in astrocytes is shown for the pathway from succinate (equal labeling of OAA at C2 and C3) and pyruvate carboxylase (unlabeled carbon entry and dilution of OAA at C2). BHB, d-β-hydroxybutyrate; OAA, oxaloacetate.

Table 1

Differential equations describing the two-compartment model

Mass balance equations
dL/dt=2CMR_glc(ox) −[V_pc +V_pdhA+V_pdhN]	L, brain lactate
dnGlu/dt=V_cyc+V_xN(KGGlu)−[V_cyc +V_xN(GluKG)]	nGlu: neuronal glutamate, 90% of total glutamate
daGlu/dt=V_cyc+V_xA(KGGlu)−[V_gln +V_xA(GluKG)]	aGlu: astrocytic glutamate, 10% of total glutamate
dGln/dt=V_gln−[V_efflux+V_cyc]	Gln: glutamine concentration, only in astrocytes
daKG/dt=V_pdhA+V_xA(GluKG)+V_AcCoA-kbA−[V_tcaANet+V_xA(KGGlu)]	aKG: astrocytic α-ketoglutarate
dnKG/dt=V_xN(GluKG)+V_pdhN+V_AcCoA-kbN−[V_xN(KGGlu)+V_tcaN]	nKG: neuronal α-ketoglutarate
daOAA/dt=0.5V_tcaANet+0.5V_tcaANet+V_pc−V_tcaA	aOAA: astrocytic oxaloacetate
dnOAA/dt=V_xNAspOAA+V_tcaN−[nV_xNOAAAsp+V_tcaN]	nOAA: neuronal oxaloacetate
nAsp/dt=V_xN(OAAAsp)−V_xN(AspOAA)	nAsp: neuronal aspartate
aAsp/dt=V_xA(OAAAsp)−V_xA(AspOAA)	aAsp: astrocytic aspartate
Isotope balance equations
dL₃/dt=CMR_glc(ox)(Brain_Glucose_1,6/Brain_Glucose)+V_dilLac(Lac₃/Lac)−[V_pc+V_dilLac+V_pdhA+V_pdhN](L₃/L)
daKG₄/dt=V_pdhA(L₃/L)+V_xA(GluKG)(aGlu₄/aGlu)+V_AcCoA-kbA(Brain_BHB_2,4/Brain_BHB)+V_dilA(Nat_Abund₀/Nat_Abund)−[V_tcaANet+V_xA(KGGlu)](aKG₄/aKG)
daKG₃/dt=V_xA(GluKG)(aGlu₃/aGlu)+V_tcaA(aOAA₃/aOAA)−[V_tcaANet+V_xA(KGGlu)](aKG₃/aKG)
dnKG₄/dt =V_xN(GluKG)(nGlu₄/nGlu)+V_pdhN(L₃/L)+V_AcCoA-kbN (Brain_BHB_2,4/Brain_BHB)−[V_xN(KGGlu)+V_tcaN](nKG₄/nKG)
dnKG₃/dt=V_xN(GluKG)(nGlu₃/nGlu)+V_tcaN(nOAA₃/nOAA)−[V_xN(KGGlu)+V_tcaN](nKG₃/nKG)
daGlu₃/dt=V_xA(KGGlu)(aKG₃/aKG)+V_cyc(nGlu₃/nGlu)−[V_gln+V_xA(GluKG)](aGlu₃/aGlu)
dGln₃/dt=V_gln(aGlu₃/aGlu)−[V_efflux+V_cyc](Gln₃/Gln)
daGlu₄/dt=V_cyc(nGlu₄/nGlu)+V_xA(KGGlu)(aKG₄/aKG)−[V_gln+V_xA(GluKG)](aGlu₄/aGlu)
dnGlu₄/dt=V_cyc(Gln₄/Gln)+V_xN(KGGlu)(nKG₄/nKG)−[V_cyc+V_xN(GluKG)](nGlu₄/nGlu)
dGln₄/dt=V_gln(aGlu₄/aGlu)−[V_efflux+V_cyc](Gln₄/Gln)
dnGlu₃/dt=V_cyc(Gln₃/Gln) +V_xN(KGGlu)(nKG₃/nKG)−[V_cyc+V_xN(GluKG)](nGlu₃/nGlu)
dnOAA₃/dt=V_xN(AspOAA)(nAsp₃/nAsp)+0.5V_tcaN(nKG₄/nKG)+0.5V_tcaN(nKG₃/nKG)−[V_xN(OAAAsp)+V_tcaN ](nOAA₃/nOAA)
daOAA₃/dt=0.5V_tcaANet(aKG₄/aKG)+0.5V_tcaANet(aKG₃/aKG)+V_xA(AspOAA)(aAsp₃/aAsp)+0.5V_pc(L₃/L)−[V_xA(OAAAsp)+V_tcaA](aOAA₃/aOAA)
dnOAA₃/dt=V_xN(AspOAA)(nAsp₃/nAsp)+0.5V_tcaN(nKG₄/nKG)+0.5V_tcaN(nKG₃/nKG)−[V_xN(OAAAsp)+V_tcaN](nOAA₃/nOAA)
daOAA₃/dt=0.5V_tcaANet(aKG₄/aKG)+0.5V_tcaANet(aKG₃/aKG)+V_xA(AspOAA)(aAsp₃/aAsp)+0.5V_pc(L₃/L)−[V_xA(OAAAsp)+V_tcaA](aOAA₃/aOAA)
Combination pools
GluTot4=aGlu+nGlu; GluTot3=aGlu+nGlu	Total glutamate concentration at C4; aGlu is set to 10% total Glu concentration, nGlu is set to 90% total Glu concentration. Total glutamate concentration at C3
Glx3=Gln+aGlu+nGlu	Sum of glutamate and glutamine at C3
Rates
CMR_glc(ox)=(V_pdhA+V_pdhN +V_pc)/2	Cerebral metabolic rate of glucose oxidation
V_cyc=0.54^∗V_tcaN	Glutamate/glutamine cycling rate
V_d=0.77 mL/g	Distribution space for glucose and BHB (assumed to be equivalent)
V_efflux=V_pc	Glutamine efflux rate balanced to pyruvate carboxylase flux
V_gln=V_cyc+V_efflux	Glutamine synthesis rate
V _pdhA	Astrocytic pyruvate dehydrogenase rate
V _pdhN	Neuronal pyruvate dehydrogenase
V_tcaA=V_pdhA+ V_AcCoA-kbA	Astroglial TCA cycle rate
V_tcaN=V_pdhN + V_AcCoA-kbN	Neuronal TCA cycle rate
V_xA(GluKG)=5 μmol/min per g	Rate of flow from glial Glu to glial KG
V_xA(KGGlu)=V_xA(GluKG)+V_pc	Rate of flow from glial KG to glial Glu
V_xN(GluKG)=V_xN(KGGlu)=5 μmol/min per g	Rate of flow between neuronal KG and neuronal Glu
V_xA(AspOAA)=V_xA(OAAAsp)=5 μmol/min per g	Rates of flow between glial aspartate and glial OAA
V_xNAspOAA=V_xNOAAAsp=5 μmol/min per g	Rates of flow between neuronal aspartate and neuronal OAA

BHB, d-β-hydroxybutyrate; KB, ketone body.

The contribution to Glu C4 enrichment from ketone bodies (BHB) is given by

f e_{s s} G l u - C 4_{k b} = f e_{s s} B H B - C 4_{A c} \times (V_{A c C o A - k b N} / V_{t c a N})

Assuming that the neuronal acetyl-CoA pool is derived only from pyruvate and BHB, the value of ‘c’ can be expressed as:

\begin{aligned} c = f e_{s s} L a c - C 3_{A c} \times (1 - V_{A c C o A - k b N} / V_{t c a N}) \\ - f e_{s s} B H B - C 4_{A c} \times V_{A c C o A - k b N} / V_{t c a N} \end{aligned}

where the value for V_AcCoA-kbN/V_tcaN was estimated from BHB-infused fasted animals and scaled downward proportionally to reflect the lower plasma BHB levels (3.8±0.2 mmol/L, ¹³C enrichment 16%±5%) in fasted [2-¹³C]acetate-infused animals.

Statistics

Metabolites concentrations, enrichments, and metabolic fluxes were reported as group average±s.d. Monte Carlo analysis of each animal's data set was performed using CWave to assess the distribution of uncertainties in the model parameters for individual animals. The uncertainties in Monte Carlo fitting were smaller than the group variability for each parameter reported in this study, indicating that uncertainties were dominated by inter animal variation.

Results

Effects of Acute [2,4-¹³C₂]-d-β-Hydroxybutyrate Infusion on Blood and Brain Ketone Bodies

Table 2 presents the concentrations and enrichments of blood ketone bodies before and during ¹³C-BHB infusion. The preinfusion plasma BHB concentration was 3±1 mmol/L, which was quickly increased to 6.5±1 mmol/L giving an average enrichment of 66%±17% (Figure 2). The BHB infusions led to a small elevation in arterial blood pH (∼0.15 Units) compared with the preinfusion baseline.

Table 2

Concentrations and ¹³C enrichments of substrates and metabolites in the plasma and cortical extracts from fasted rats at baseline (plasma) and after [2,4-¹³C₂]-d-BHB infusion (plasma and brain)

	Baseline	After BHB infusion (end point)
	Concentration (mmol/L)	Concentration (mmol/L)	¹³C enrichment (%)
Blood plasma
BHB	3.0±1.0	6.5±1.0	66±17
AcAc	1.2±0.6	2.9±1.6	58±4
Acetone	0.20±0.08	0.6±0.2	40±4
Acetate	0.14±0.02	0.3±0.2	1.6±0.8
Glucose	5.5±1.8	6.9±2.4	5.8±2.2
Lactate	0.9±0.3	2.7±0.3	5.2±2.2
Cerebral cortex
BHB	0.26, 0.56^a	0.7±0.3	64±7 (C4)
Acetone^b	NA	0.025±0.004	39±4 (C1,3)
Glucose	0.96, 1.81^a	2.7±0.6	3.7±0.2 (C1,6)
Lactate	1.7, 1.6^a	2.8±0.5	4.5±1.1 (C3)
Alanine	0.47, 0.55^a	0.8±0.2	6.1±1.7 (C3)
Aspartate	3.5, 2.8^a	3.2±0.8	14.9±2.7 (C3)
Glutamate	12.4, 11.7^a	11.9±0.3	20.2±3.3 (C3)
			30.6±2.9 (C4)
Glutamine	6.65, 6.58^a	7.9±1.2	16.7±4.2 (C3)
			25.0±2.5 (C4)
GABA	1.35, 1.44^a	1.4±0.5	11.9±2.2 (C2)
			6.90±2.2 (C3)

BHB, d-β-hydroxybutyrate; LC, linear combination; NA, not available.

Cortical metabolite levels measured in extracts from two halothane-anesthetized animals fasted for 36 hours and prepared similarly but not infused with BHB. In these rats, the average concentration of plasma BHB was 3.9 mmol/L and that of lactate was 0.61 mmol/L. All other values represent mean±s.d. Enrichment (%) reflects ¹³C excess percentage enrichment above the natural abundance of 1.1%. All metabolites, except acetone, were measured in the end-point extract.

Acetone concentration was determined from the LC model fitted in vivo spectrum.

Figure 2

Time courses of BHB concentrations in the plasma and brain and ¹³C enrichment (plasma). BHB, d-β-hydroxybutyrate.

Blood levels of other ketone bodies were also increased and enriched with ¹³C during ¹³C-BHB infusion. Acetoacetate comprised ∼40% to 44% of the plasma BHB level (Table 2) and was similarly enriched, but was not detected in brain spectra in vivo or in the extract, likely because of both low brain concentration and overlap with GABA-C2. Plasma levels of acetone increased ∼3-fold during ¹³C-BHB infusion and were highly enriched with ¹³C (∼40%). Acetone was observed in plasma and brain spectra in vivo, but not in brain extracts because of its high volatility and loss during lyophilization. Plasma acetate levels increased similarly to BHB and acetoacetate (2.1-fold) during ¹³C-BHB infusion, although ¹³C enrichment remained relatively low (1.6%, Table 2), indicating limited conversion of the infused ¹³C-BHB to free acetate.

Enrichment of Brain Amino Acids From [2,4-¹³C₂]-d-β-Hydroxybutyrate

d-β-Hydroxybutyrate-C4 was readily detected in the brain ¹H-[¹³C] difference spectra in vivo within the first 5 minutes of ¹³C-BHB infusion (Figure 3A). Extensive incorporation of the ¹³C label into the major brain amino-acid pools was seen (Figures 3A and 3B), appearing initially in glutamate (Glu)-C4, followed by glutamine (Gln)-C4, glutamate and glutamine (Glx)-C3, Glx-C2, and aspartate (Asp)-C3. Comparing the ¹H-[¹³C] difference spectrum acquired at 96 minutes (Figure 3B) with the spectrum representing the sum of ¹²C and ¹³C (Figure 3C) shows the high enrichment of glutamate and glutamine from ¹³C-BHB. The dynamic enrichment pattern is nearly identical to that observed when [1-¹³C]glucose is the infused substrate (e.g., see the study by Pan et al (2002)). In addition to the enriched amino acids, ¹³C-labeled acetone was also observed during ¹³C-BHB infusion (Figure 3B; Supplementary Figure S1A), a product not observed during infusions of other substrates, such as [1,6-¹³C₂]glucose or [2-¹³C]acetate (Supplementary Figure S1B and C).

Figure 3

Spatially localized in vivo¹H-[¹³C] spectra of the rat cerebrum acquired during an intravenous infusion of [2,4-¹³C₂]-d-BHB. (A) Time course of ¹H-[¹³C] difference spectra acquired serially showing ¹³C labeling of brain amino acids during the [2,4-¹³C₂]-d-BHB infusion. The time of spectrum acquisition (midpoint, in minutes) is shown on the right. (B) ¹H-[¹³C] difference spectrum reflecting ¹H atoms bound to ¹³C atoms obtained by the difference of two acquired spectra with and without a ¹³C inversion pulse as described in the ‘Materials and methods’ section. (C) ¹H-[¹³C] spectrum of the sum of ¹²C and ¹³C resonances (total intensity). Carbonyl carbons are not detected in the ¹H-[¹³C] difference spectra because of the absence of covalently bonded protons. BHB infusion begins at t=0. Acquisition parameters: relaxation delay, 2.5 seconds; number of scans, 256; number of data points, 1,024, spectral volume, 6 × 6 × 6 mm³. Definitions: Cr_tot, total creatine; NAA, N-acetylaspartate; Glu, glutamate; Glx, glutamate+glutamine; Ala, alanine; Lac, lactate. BHB, d-β-hydroxybutyrate.

Table 2 depicts the total concentrations and end-point ¹³C enrichments of cortical amino acids measured after 100 minutes of infusion of [2,4-¹³C₂]-d-BHB. Inspection of the average end-point enrichments for Glu-C4 (30.6%), Gln-C4 (25%), and Asp-C3 (15%) shows values that are similar to those obtained from [1,6-¹³C]glucose or [U-¹³C]glucose for comparable plasma enrichment; however, GABA-C2 (11.9%) was much less. The steady-state GABA-C2/Glu-C4 ratio was only 0.40, which is considerably lower than that observed from ¹³C-labeled glucose infused under the same anesthetic condition (halothane) (0.74, Chowdhury et al, 2007; 0.83, van Eijsden et al, 2010). As GABA levels (1.4 μmol/g) are similar to cortical levels determined in ¹³C-glucose-infused, halothane-anesthetized rats (1.2 μmol/g, van Eijsden et al, 2010), the lower ¹³C enrichment in GABA-C2 from ¹³C-BHB in this study can be ascribed to a greater contribution of glucose (unlabeled dilution) to oxidative metabolism in GABA neurons.

Assessment of Metabolic Fluxes

Figure 4 depicts time courses of cortical ¹³C enrichments of Glu-C4 and Gln-C4 for a typical animal during [2,4-¹³C₂]-d-BHB infusion. The solid lines reflect the best fits of the constrained two-compartment (neuron–astrocyte) metabolic model (Figure 1 and Table 1) to the time courses, which yielded the absolute rate estimates of ketone body and glucose oxidation in neurons and astrocytes. The metabolic rates are summarized in Table 3. d-β-Hydroxybutyrate oxidation accounted for a substantial fraction (40%±5%) of the overall substrate oxidation of neurons and astrocytes. d-β-Hydroxybutyrate was oxidized quantitatively to a greater extent in neurons, however, accounting for ∼70% of total ketone body consumption (neurons, 0.31±0.16 μmol/g per min; astrocytes, 0.14±0.09 μmol/g per min). Within the two different neural cell types, BHB contributed a slightly larger fraction of the total substrate oxidation in neurons (neurons, ∼52%; astrocytes, ∼33%).

Table 3

Estimates of cerebral metabolic fluxes (μmol/g per min) during hyperketonemia and [2,4-¹³C₂]-d-BHB infusion determined by a constrained two-compartment (neuron–astrocyte) metabolic model

	CMR _glc(ox)	CMR _kb	V_AcCoA-kbN	V_AcCoA-kbA	V_pdhA	V_pdhN	V_tcaN	V_tcaA	V_Gln	V_cyc
Mean	0.33	0.22	0.31	0.14	0.29	0.29	0.60	0.42	0.40	0.32
Group s.d.	(0.13)	(0.04)	(0.16)	(0.09)	0.08	0.18	(0.32)	(0.13)	(0.21)	(0.17)
MCSD	(0.03)	(0.01)	(0.02)	(0.02)	0.04	0.03	(0.05)	(0.05)	(0.03)	(0.02)

BHB, d-β-hydroxybutyrate; MCSD, Monte-Carlo Standard Deviation.

Fluxes are given in units of μmol/g per min. Values represent mean±s.d. of six animals. The metabolic model was fitted to the time courses for each animal individually and the parameter estimates (best fits) were averaged to give the values shown. V_cyc/V_tcaN was constrained to the value of the ratio (0.54) calculated from Glu-C4 and Gln-C4 enrichments measured at isotopic steady-state during an infusion of [2-¹³C]acetate for a separate group of 36 hour-fasted rats (see the ‘Materials and methods’ section). V_pc was constrained to 0.2 × V_gln (Sibson et al, 2001). MCSD is the mean s.d. from the 20-step Monte Carlo analysis of 6 animals, providing a measure of the quality of individual fits.

Figure 4

Time courses of ¹³C enrichments of Gln-C4, Glu-C4, and Glx3, and best fits of the metabolic model for a 36 hour-fasted rat during [2,4-¹³C₂]-BHB infusion. The brain amino-acid ¹³C enrichments reflect the measured values (not normalized by plasma BHB ¹³C enrichment). The average blood BHB ¹³C enrichment was 65% for this animal. BHB, d-β-hydroxybutyrate.

The measured neuronal TCA cycle rate in 36 hour-fasted rats is similar to that seen in overnight-fasted, halothane-anesthetized rats infused with [1,6-¹³C] or [U-¹³C]glucose (0.47 to 0.55 μmol/g per min, van Eijsden et al, 2010; Jiang et al, 2009; Patel et al, 2004). In the latter studies, glucose accounts for >90% of oxidized substrate, such that V_pdhN≈V_tcaN. In contrast to glucose-infused rats, V_pdhN (0.29 μmol/g per min) was ∼40% to 50% lower in hyperketonemic rats indicating that BHB oxidation is compensated by an equal reduction in oxidation of glucose-derived acetyl-CoA.

Effects of Parameter Constraints on the Calculated Fluxes

The reliability of the estimated value of the glutamate/glutamine cycling flux (V_cyc) when iterated as a free parameter depends on the difference in the time courses of Glu-C4 and Gln-C4 (Shen et al, 2009). In contrast to the well-separated Glu-C4 and Gln-C4 enrichment curves seen in anesthetized rats infused with [1-¹³C]glucose (Patel et al, 2005), the enrichment curves were closer together in [2,4-¹³C]BHB-infused rats, decreasing reliability in the value of V_cyc as an iterated parameter. To improve reliability in flux estimates, the model fits were constrained by the ratio, V_cyc/V_tcaN, determined in 36 hour-fasted rats infused with the astroglial substrate, [2-¹³C]acetate (see the ‘Materials and methods’ section) (Supplementary material, Table S1).

Fitting the metabolic fluxes with the V_cyc/V_tcaN ratio constrained (0.54) compared with V_cyc freely iterated improved the individual fits, reducing %MCSD (% Monte Carlo Standard Deviation) of V_cyc ∼40% without significantly altering variance in V_tcaN and V_AcCoA-kb, effects consistent with previous analysis (Shen et al, 2009).

[2,4-¹³C]BHB labels the TCA cycle and associated amino acids through [2-¹³C]acetyl-CoA, and is relatively insensitive to glial anaplerosis (pyruvate carboxylase flux). Therefore, V_pc was constrained to a value equal to 0.2 × V_gln based on a previous study of anesthetized rats infused with [2-¹³C]glucose (Sibson et al, 2001). To assess the sensitivity of the calculated fluxes on the value of this parameter, a sensitivity analysis was performed by fitting the metabolic model to the ¹³C time courses using different V_pc/V_gln ratio values, 50% below and 50% above the assumed value (Supplementary Material, Table S2). The analysis estimates the potential error in the calculated fluxes from their nominal values should the true value of the parameter be lower or higher than assumed. Relatively small differences from the nominal values were seen for lower values (V_pc/V_gln=0.1, −3% to −10%), but differences became larger for higher values (V_pc/V_gln=0.3, −44% to +35%), whereas fit quality was poor.

Discussion

In this study, we measured the contributions of ketone bodies and glucose to neuronal and astroglial oxidation in anesthetized rats made hyperketonemic by prolonged (36 hours) fasting and infused acutely with [2,4-¹³C]-BHB. The following main conclusions can be drawn: (1) BHB oxidation in the fasted anesthetized rat cortex is extensive, accounting for ∼40% of total TCA cycle flux; (2) quantitatively, BHB oxidation is greater in neurons (70% of total ketone consumption) than in astrocytes; and (3) comparisons with glucose-infused rats from previous studies show that ketone body oxidation is compensated by an equal reduction in glucose oxidation.

Comparison of Present Results With Previous Studies

Prolonged fasting is well known to enhance ketone body transport (Gjedde and Crone, 1975; Hasselbalch et al, 1996). The prolonged fast effectively produced a stable steady state of hyperketonemia before ¹³C-labeled BHB infusion, thus avoiding the major brain metabolic/regulatory shifts in transitioning between glucose- and BHB-fed conditions. The brain-to-blood plasma ratio for BHB in 36 hour-fasted rats of 0.11±0.04 agrees well with values from 48 hour-fasted rats (0.12, Hawkins et al (1971)), and is higher than that seen in fed rats infused acutely with ketone bodies (0.075, Hawkins et al (1971)), consistent with a fasting-induced enhancement in ketone body transport.

The rate of cortical ketone body oxidation, CMR_kb (=1/2V_AcCoA-kb) of 0.22±0.04 μmol/g per min, can be compared with previous estimates based on cerebral arteriovenous difference or ¹⁴C-autoradiography. Assuming a net extraction ratio for BHB of ∼0.04 to 0.07 (Hawkins et al, 1971; Daniel et al, 1971; Gjedde and Crone, 1975; Conn et al, 1983) and for acetoacetate of ∼0.1 (Hawkins et al, 1971), and a brain blood flow of ∼0.62 mL/g per min (Dahlquist and Persson, 1976), the corresponding rates per unit blood concentration (mmol/L) is given by the product of the extraction ratio and cerebral blood flow, yielding ∼0.02 to 0.04 and ∼0.06 μmol/g per min per mmol/L, respectively. For plasma BHB and acetoacetate concentrations of 6.5 and 2.9 mmol/L, respectively (Table 1), CMR_kb would be ∼0.30 to 0.43 μmol/g per min. This value likely represents an upper estimate because extraction is assumed to be unidirectional with ketone bodies metabolized immediately upon entry. However, brain concentration of BHB (after correction for an assumed 3% blood volume) is not zero but is substantial (0.51 mmol/L), suggesting that net extraction (metabolism) is less than the values used above. Using clearance data from the ¹⁴C-autoradiography study of Hawkins et al (1986) for the parietal cortex of 2 day-starved unanesthetized rats, ketone body influx (μmol/g per min) can be computed from the BHB clearance rate (0.025 mL/g per min), the ratio of acetoacetate and BHB clearance (1.46), and the present concentrations of BHB (6.5 mmol/L) and acetoacetate (2.9 mmol/L), and is given by: CMR_kb=CMR_BHB+CMR_AcAc=(0.025 × 6.5)+(0.025 × 1.46 × 2.9)=0.27 (±0.08) μmol/g per min. Considering the differences between methods, conditions (awake versus anesthetized), brain tissue represented in the measurements (whole brain versus cortex), and rat strains/gender (Wistar females and Long-Evans males versus Sprague–Dawley males), the Magnetic Resonance Spectroscopy (MRS)-derived value for ketone body consumption in vivo is in reasonably good agreement with other approaches.

β-Hydroxybutyrate Oxidation in Cortical Neurons and Astrocytes

Glutamate is concentrated primarily in neurons, whereas glutamine is produced and concentrated in astrocytes. [2,4-¹³C₂]d-β-hydroxybutyrate enters the TCA cycle as acetyl-CoA at C2, labeling citrate-C4 followed by α-ketoglutarate at C4, which through mitochondrial/cytoplasmic exchange with glutamate enriches Glu-C4 in the large cytoplasmic pool. Glutamate labeled in neurons is released during synaptic activity, taken up by astrocytes, and metabolized to glutamine. Thus, the relative kinetics of Glu-C4 and Gln-C4 labeling during an infusion of a ¹³C-labeled substrate which labels acetyl-CoA at C2 (e.g., [1,6-¹³C₂]glucose or [2-¹³C]-acetate) reflects the relative preference of neurons and astrocytes for that substrate. During [2,4-¹³C]-d-BHB infusion, the ¹³C enrichment of Glu-C4 exceeded Gln-C4 at all times. This is consistent with findings using tracer levels of [3-¹⁴C]BHB in unanesthetized 48-hour-fasted and ketotic adult rats (Cremer, 1971) and in preweanling rats, which are normally ketotic (DeVivo et al, 1975), and interpreted as BHB oxidation in the large glutamate pool associated with neurons. A study of the occipital cortex of overnight-fasted, awake healthy human subjects using ¹³C MRS with [2,4-¹³C]-d-BHB infusion also shows the same labeling pattern, similar to that produced from [1-¹³C]glucose, and attributed to neuronal oxidation (Pan et al, 2002).

However, our findings contrast with those of Kunnecke et al (1993) who reported a higher labeling ratio in brain Gln-C4 relative to Glu-C4 (Gln/Glu >1) during acute hyperketonemia in fed rats receiving infusions of [U-¹³C]BHB, and was interpreted as a greater metabolism of ketone bodies in astrocytes than in neurons. It is not clear whether dietary status alone (fed versus fasted) might produce this effect. Enhanced glutamine synthesis has been reported in mice fed the ketogenic diet after intraperitoneal injection of [1,2-¹³C]acetate and [1-¹³C]glucose (Melo et al, 2006; Yudkoff et al, 2005), suggesting that astrocytes may adapt to prolonged ketosis, but this condition would have been anticipated in fasted rats rather than in fed rats. The influence of dietary status (fed, fasted, ketogenic diet) and species on astrocyte metabolism during hyperketonemia in vivo merits further study.

Effects of Hyperketonemia on Glucose Utilization in the Cerebral Cortex

The neuronal TCA cycle rate of hyperketonemic-fasted animals in our study (0.60±0.32 μmol/min per g) is similar to our previously reported values from overnight-fasted, halothane-anesthetized rats infused with [1,6-¹³C]glucose, as measured from a comparable brain region and spectroscopic volume (0.52 to 0.53 μmol/min per g (Patel et al, 2004; van Eijsden et al, 2010)). This observation is consistent with previous studies of rats showing that brain oxygen consumption (which is proportional to TCA cycle activity) is unaffected by hyperketonemia whether by acute BHB infusion (Linde et al, 2006) or by starvation for 48 to 72 hours (Dahlquist and Persson, 1976; Hawkins, 1971).

As total rates of substrate oxidation (TCA cycle flux) in neurons and astrocytes seemed similar to their respective values in nonketonemic, ¹³C-glucose-infused, anesthetized rats (Patel et al, 2005), we conclude that BHB oxidation was compensated by an equivalent reduction in acetyl-CoA oxidation from glucose. Previous studies have reported inconsistent findings regarding the effects of hyperketonemia on glucose utilization. In an early study of brain slices/minces, Openshaw and Bortz (1968) reported that ketone body elevation decreased glucose oxidation, whereas others reported increased lactate formation with no change in glucose oxidation (Ito and Quastel, 1970; Rolleston and Newsholme, 1967). Studies reporting brain glucose utilization by arterio/venous difference (Linde et al, 2006) or ¹⁴C-2-deoxyglucose phosphorylation (Corddry et al, 1982; Crane et al, 1985) in rats during hyperketonemia, whether induced by fasting (2 to 3 days) or by acute BHB infusion, consistently show no change in CMR_glc compared with the fed condition, unless fasting is more prolonged when a decrease in CMR_glc occurs (Crane et al, 1985). The findings that increased oxidation of ketone bodies occurs without altering either glucose or oxygen consumption suggest that glucose metabolism during hyperketonemia is diverted to lactate production and efflux to the blood, which may occur transiently, as reported by Hawkins et al (1971).

Brain lactate was elevated in end-point extracts when compared with rats fasted for a comparable time (36 hours) but not infused with BHB (Table 2), although animal numbers were too few at baseline for statistical assessment. As plasma lactate levels also increased during ¹³C-BHB infusion, we cannot say whether the higher brain lactate reflects blood level changes, or possibly reduced intracellular clearance caused by the reduction in pyruvate/lactate oxidation and efflux as described by Pan et al (2000) in 2 to 3 day-fasted human subjects. As a cautionary note, extract measurements are susceptible to postmortem effects that can increase brain lactate, and whereas rats in our study were ventilated during brain freezing to reduce the likelihood of agonal change, this possibility cannot be excluded entirely.

Few in vivo studies are available that report glucose oxidation, as opposed to glucose uptake (arterio/venous difference) or phosphorylation (¹⁴C-2-deoxyglucose autoradiography). One exception is the study by Mans et al (1987), who found a small decrease in glucose oxidation (∼12% averaged over the whole brain) in unanesthetized rats fasted for 2 days compared with fed rats. These authors used quantitative autoradiography with short time infusion (5 minutes) of [6-¹⁴C]glucose, which is metabolized and trapped mainly as ¹⁴C-labeled amino acids, a technique most closely related to the NMR approach in this study. The larger reduction in glucose oxidation assessed in this study would be expected based on the much greater plasma BHB level (6.5 mmol/L) compared with the study by Mans et al (0.85 mmol/L).

Rodents subjected to prolonged hyperketonemia through a high-fat (ketogenic) diet show similar effects on glucose metabolism to those seen after short periods of fasting (<3 days), in that glucose oxidation is reduced but glucose uptake (phosphorylation) is unaltered. al-Mudallal et al (1995) found no alteration in regional CMR_glc based on ¹⁴C-2-deoxyglucose phosphorylation in rats fed a ketogenic diet for 6 to 7 weeks. In contrast, in mice fed a ketogenic diet for 2 weeks followed by injection of [1-¹³C]glucose, significant reductions were reported in ¹³C labeling of brain metabolites and amino acids (Yudkoff et al, 2005; Melo et al, 2006).

Interestingly, in contrast to findings in rodents, prolonged fasting or acute BHB infusion in humans is associated with reduced brain glucose utilization (26% to 33%) as measured by positron emission tomography with [¹⁸F]2-fluoro-2-deoxy-d-glucose or arterio/venous difference methods (Hasselbalch et al, 1996).

Potential Impact of Other ¹³C-Labeled Ketones and Nonketone Substrates on the Analysis

The ¹³C enrichments of acetoacetate and acetone were not considered in the present analysis, although both were highly enriched (Table 2). Acetoacetate is an intermediate in BHB catabolism and is in fast equilibrium with BHB; hence, its time course of labeling would be expected to be proportional to BHB. As acetoacetate was not detected in the brain spectra, and the transport kinetics of acetoacetate across the blood–brain barrier is uncertain, the use of blood or brain acetoacetate levels as a driver was precluded. We also did not account for the labeled acetone in the metabolic model. As acetone is highly volatile, it is likely to be exhaled and may not contribute significantly as a fuel substrate. However, acetone can be metabolized by the liver and labels plasma glucose in fasted humans (Reichard et al, 1979) and in rats under normal and diabetic ketotic conditions, as well as BHB in ketosis (Kosugi et al, 1986).

Any labeling of BHB-C4,2 by acetone-C1,3 would not be distinguished from the infused ¹³C-BHB used as the input.

Blood glucose ¹³C enrichment (5.8% in C1) was detected in fasted/¹³C-BHB-infused animals at ∼90 minutes, which would not be distinguished from the ¹³C-BHB because both substrates lead to the same labeling pattern for acetyl-CoA. Although we cannot specifically address its origin, we included the labeled glucose as an additional driver (input) in the modeling (Table 1) by assuming that glucose enrichment increased linearly over the period of the ¹³C-BHB infusion and that glucose-C1 and glucose-C6 were equally enriched. Although acetyl-CoA derived from glucose represented ∼56% of total acetyl-CoA (the rest supplied by ketone bodies), the low plasma glucose enrichment of 5.8% would be expected to result in a relatively small contribution (0.56 × 0.058 × 100=3.2%) to the glutamate-C4 end-point enrichment at isotopic steady state.

Ketone Body Utilization in Relation to Aspartate and GABA

The steady-state labeling of aspartate (Asp) and GABA relative to glutamate or glutamine from [2,4-¹³C]-d-BHB (Table 2) is much less than is seen for nonketonemic animals prepared similarly but infused with [1-¹³C], [1,6-¹³C], or [U-¹³C]glucose (Tyson et al, 2003; van Eijsden et al, 2010). Values of the steady-state enrichment ratios of Asp-C3/Glu-C4 (0.49) and GABA-C2/Glu-C4 (0.39) were lower than respective values seen in glucose-infused rats (>0.75). This observation indicates that Asp and GABA do not share the same acetyl-CoA precursor pool with the large glutamate pool (glutamatergic neurons) in terms of relative flows of ketone bodies and glucose. DeVivo et al (1975) compared ¹⁴C labeling of brain amino acids in brain's of developing rats from tracer doses of [2-¹⁴C]glucose or [3-¹⁴C]BHB, finding a lower GABA/Glu ¹⁴C-specific activity ratio after postnatal day 15, and concluded that BHB was less effective than glucose as a substrate for GABA synthesis after this postnatal age. In our study, the similar percentage reductions in Asp and GABA labeling from [2,4-¹³C]-d-BHB compared with [1,6-¹³C]glucose, could be explained if Asp was more concentrated in GABAergic neurons than in astrocytes. Furthermore, because steady-state GABA labeling from ¹³C-BHB appeared less than would be expected from ¹³C-glucose, our results indicate that glucose is preferred over BHB as a fuel for GABAergic neurons. Reduced labeling of Asp was also reported in a ¹³C MRS study of human subjects infused with [2,4-¹³C]-d-BHB (Pan et al, 2002). Steady-state ¹³C labeling of Asp (C3) relative to Glu (C4,C3) was much less than expected had Asp and Glu labeling from BHB occurred in the same cellular (neuronal) pool, suggesting that Asp was mostly present in nonglutamatergic cells, possibly astrocytes or GABAergic neurons. These findings concur, suggesting that Asp may be enriched in GABAergic neurons.

Conclusions

In summary, our results show that in anesthetized rats fasted for 36 hours, ketone bodies can support as much as 40% of total substrate oxidation in the cerebral cortex. Neurons and astrocytes oxidize BHB, although neurons consume the most and in a pattern similar to glucose. The oxidation of ketone bodies is compensated by reduced oxidation of glucose resulting in an apparently unaltered TCA cycle flux. Further experiments are required to define the range over which ketone body oxidation can be increased. This is important because of recent work indicating that transport and utilization of acetate (and possibly other monocarboxylic acids) is increased in humans with type 1 diabetes (Mason et al, 2006). The capacity of brain cells to oxidize substrates other than glucose (e.g., acetate, ketone bodies, free fatty acids, etc.) could provide significant therapeutic opportunities to enhance neuronal and astroglial function during times of hypoglycemic stress.

Footnotes

Acknowledgements

The authors thank Ms Bei Wang for assistance in animal preparation.

The authors declare no conflict of interest.

Notes

Supplementary Information accompanies the paper on the Journal of Cerebral Blood Flow & Metabolism website ()

References

al-Mudallal

Levin

Lust

Harik

(1995) Effects of unbalanced diets on cerebral glucose metabolism in the adult rat. Neurology 45:2261–5

Chowdhury

Gupta

Gibson

Patel

Behar

(2007) Altered cerebral glucose and acetate metabolism in succinic semialdehyde dehydrogenase-deficient mice: evidence for glial dysfunction and reduced glutamate/glutamine cycling. J Neurochem 103:2077–91

Conn

Fell

Steele

(1983) Characterization of α-keto acid transport across blood–brain barrier in rats. Am J Physiol 245:E253–60

Corddry

Rapoport

London

(1982) No effect of hyperketonemia on local cerebral glucose utilization in conscious rats. J Neurochem 38:1637–41

Crane

Pardridge

Braun

Oldendorf

(1985) Two-day starvation does not alter the kinetics of blood–brain barrier transport and phosphorylation of glucose in rat brain. J Cereb Blood Flow Metab 5:40–6

Cremer

(1971) Incorporation of label from D-β-hydroxy-(¹⁴C)butyrate and (3-¹⁴C)acetoacetate into amino acids in rat brain in vivo. Biochem J 122:135–8

Dahlquist

Persson

(1976) The rate of cerebral utilization of glucose, ketone bodies, and oxygen: a comparative in vivo study of infant and adult rats. Pediatr Res 10:910–7

Daniel

Love

Moorehouse

Pratt

Wilson

(1971) Factors influencing utilisation of ketone-bodies by brain in normal rats and rats with ketoacidosis. Lancet 2:637–8

de Graaf

(2007) In Vivo NMR Spectroscopy/Principals and Techniques. John Wiley & Sons, Ltd: Hoboken, NJ, USA

10.

DeVivo

Malas

Leckie

(1975) Starvation and seizures. Observation on the electroconvulsive threshold and cerebral metabolism of the starved adult rat. Arch Neurol 32:755–60

11.

Gjedde

Crone

(1975) Induction processes in blood–brain transfer of ketone bodies during starvation. Am J Physiol 229:1165–9

12.

Gruetter

(1993) Automatic localized in vivo adjustment of all first- and second-order shim coils. Magn Reson Med 29:804–11

13.

Hasselbalch

Madsen

Hageman

Olsen

Justesen

Holm

Paulson

(1996) Changes in cerebral blood flow and carbohydrate metabolism during acute hyperketonemia. Am J Physiol 270: E746–51

14.

Hawkins

(1971) Uptake of ketone bodies by rat brain in vivo. Biochem J 121:17P

15.

Hawkins

Mans

Davis

(1986) Regional ketone body utilization by rat brain in starvation and diabetes. Am J Physiol 250:E169–78

16.

Hawkins

Williamson

Krebs

(1971) Ketone-body utilization by adult and suckling rat brain in vivo. Biochem J 122:13–8

17.

Ito

Quastei

(1970) Acetoacetate metabolism in infant and adult rat brain in vitro. Biochem J 116: 641–55

18.

Jiang

Herzog

Mason

de Graaf

Rothman

Sherwin

Behar

(2009) Recurrent antecedent hypoglycemia alters neuronal oxidative metabolism in vivo. Diabetes 58:1266–74

19.

Kosugi

Chandramouli

Kumaran

Schumann

Landau

(1986) Determinants in the pathways followed by the carbons of acetone in their conversion to glucose. J Biol Chem 261:13179–81

20.

Kunnecke

Cerdan

Seelig

(1993) Cerebral metabolism of [1,2-¹³C₂]glucose and [U-¹³C₄]3-hydroxybutyrate in rat brain as detected by ¹³C NMR spectroscopy. NMR Biomed 6:264–77

21.

LaManna

Salem

Puchowicz

Erokwu

Koppaka

Flask

Lee

(2009) Ketones suppress brain glucose consumption. Adv Exp Med Biol 645:301–6

22.

Lebon

Petersen

Cline

Shen

Mason

Dufour

Behar

Shulman

Rothman

(2002) Astroglial contribution to brain energy metabolism in humans revealed by ¹³C nuclear magnetic resonance spectroscopy: elucidation of the dominant pathway for neurotransmitter glutamate repletion and measurement of astrocytic oxidative metabolism. J Neurosci 22:1523–31

23.

Linde

Hasselbalch

Topp

Paulson

Madsen

(2006) Global cerebral blood flow and metabolism during acute hyperketonemia in the awake and anesthetized rat. J Cereb Blood Flow Metab 26:170–80

24.

Mans

Davis

Hawkins

(1987) Regional brain glucose use in unstressed rats after two days of starvation. Metab Brain Dis 2:213–21

25.

Mason

Falk Petersen

de Graaf

Kanamatsu

Otsuki

Shulman

Rothman

(2003) A comparison of ¹³C NMR measurements of the rates of glutamine synthesis and the tricarboxylic acid cycle during oral and intravenous administration of [1-¹³C]glucose. Brain Res Brain Res Protoc 10:181–90

26.

Mason

Petersen

Lebon

Rothman

Shulman

(2006) Increased brain monocarboxylic acid transport and utilization in type 1 diabetes. Diabetes 55:929–34

27.

Melo

Nehlig

Sonnewald

(2006) Neuronalglial interactions in rats fed a ketogenic diet. Neurochem Int 48:498–507

28.

Openshaw

Bortz

(1968) Oxidation of glucose, acetoacetate, and palmitate in brain mince of normal and ketotic rats. Diabetes 17:90–5

29.

Pan

de Graaf

Petersen

Shulman

Hetherington

Rothman

(2002) [2,4-¹³ C₂]-β-Hydroxybutyrate metabolism in human brain. J Cereb Blood Flow Metab 22:890–8

30.

Pan

Rothman

Behar

Stein

Hetherington

(2000) Human brain beta-hydroxybutyrate and lactate increase in fasting-induced ketosis. J Cereb Blood Flow Metab 20:1502–7

31.

Pan

Telang

Lee

de Graaf

Rothman

Stein

Hetherington

(2001) Measurement of β-hydroxybutyrate in acute hyperketonemia in human brain. J Neurochem 79:539–44

32.

Patel

de Graaf

Mason

Kanamatsu

Rothman

Shulman

Behar

(2004) Glutamatergic neurotransmission and neuronal glucose oxidation are coupled during intense neuronal activation. J Cereb Blood Flow Metab 24:972–85

33.

Patel

de Graaf

Mason

Rothman

Shulman

Behar

(2005) The contribution of GABA to glutamate/glutamine cycling and energy metabolism in the rat cortex in vivo. Proc Natl Acad Sci USA 102:5588–93

34.

Patel

Rothman

Cline

Behar

(2001) Glutamine is the major precursor for GABA synthesis in rat neocortex in vivo following acute GABA-transaminase inhibition. Brain Res 919:207–20

35.

Reichard

Jr Haff

Skutches

Paul

Holroyde

Owen

(1979) Plasma acetone metabolism in the fasting human. J Clin Invest 63:619–26

36.

Rolleston

Newsholme

(1967) Effects of fatty acids, ketone bodies, lactate and pyruvate on glucose utilization by guinea-pig cerebral cortex slices. Biochem J 104:519–23

37.

Shen

Rothman

Behar

(2009) Determination of the glutamate-glutamine cycling flux using two-compartment dynamic metabolic modeling is sensitive to astroglial dilution. J Cereb Blood Flow Metab 29: 108–18

38.

Sibson

Mason

Shen

Cline

Herskovits

Wall

Behar

Rothman

Shulman

(2001) In vivo ¹³C NMR measurement of neurotransmitter glutamate cycling, anaplerosis and TCA cycle flux in rat brain during [2-¹³C]glucose infusion. J Neurochem 76:975–89

39.

Storm-Mathisen

Leknes

Bore

Vaaland

Edminson

Haug

Ottersen

(1983) First visualization of glutamate and GABA in neurones by immunocytochemistry. Nature 301:517–20

40.

Tyson

Gallagher

Sutherland

(2003) ¹³C-labeled substrates and the cerebral metabolic compartmentalization of acetate and lactate. Brain Res 992:43–52

41.

van Eijsden

Behar

Mason

Braun

de Graaf

(2010) In vivo neurochemical profiling of rat brain by ¹H-[¹³C] NMR spectroscopy: cerebral energetics and glutamatergic/GABAergic neurotransmission. J Neurochem 112:24–33

42.

Yudkoff

Daikhin

Nissim

Horyn

Lazarow

Luhovyy

Wehrli

Nissim

(2005) Response of brain amino acid metabolism to ketosis. Neurochem Int 47:119–28

Supplementary Material

Please find the following supplemental material available below.

For Open Access articles published under a Creative Commons License, all supplemental material carries the same license as the article it is associated with.

For non-Open Access articles published, all supplemental material carries a non-exclusive license, and permission requests for re-use of supplemental material or any part of supplemental material shall be sent directly to the copyright owner as specified in the copyright notice associated with the article.

0.00 MB

0.20 MB

Cortical Substrate Oxidation during Hyperketonemia in the Fasted Anesthetized Rat in Vivo

Abstract

Keywords

Introduction

Materials and methods

Animal Preparation

In Vivo Nuclear Magnetic Resonance Spectroscopy

Preparation of Blood Plasma and Brain Extracts and Nuclear Magnetic Resonance Spectroscopy

Description of the Metabolic Model Incorporating d-β-Hydroxybutyrate Oxidation

Statistics

Results

Effects of Acute [2,4-13C2]-d-β-Hydroxybutyrate Infusion on Blood and Brain Ketone Bodies

Enrichment of Brain Amino Acids From [2,4-13C2]-d-β-Hydroxybutyrate

Assessment of Metabolic Fluxes

Effects of Parameter Constraints on the Calculated Fluxes

Discussion

Comparison of Present Results With Previous Studies

β-Hydroxybutyrate Oxidation in Cortical Neurons and Astrocytes

Effects of Hyperketonemia on Glucose Utilization in the Cerebral Cortex

Potential Impact of Other 13C-Labeled Ketones and Nonketone Substrates on the Analysis

Ketone Body Utilization in Relation to Aspartate and GABA

Conclusions

Footnotes

Acknowledgements

Notes

References

Supplementary Material

Effects of Acute [2,4-¹³C₂]-d-β-Hydroxybutyrate Infusion on Blood and Brain Ketone Bodies

Enrichment of Brain Amino Acids From [2,4-¹³C₂]-d-β-Hydroxybutyrate

Potential Impact of Other ¹³C-Labeled Ketones and Nonketone Substrates on the Analysis