Lactate Transport and Signaling in the Brain: Potential Therapeutic Targets and Roles in Body—Brain Interaction

Introduction

l-lactate, pyruvate, and ketone bodies like β-hydroxybutyrate and acetoacetate are monocarboxylates transported across different brain cell membranes by monocarboxylate transporters (MCTs).^{1, 2}l-lactate is the MCT substrate that is most abundant in the brain, and fluctuates the most in concentration. As the MCTs mediate facilitative transport, they serve to equilibrate substrate concentrations across cell membranes, the concentration gradients of substrate and cotransported ion being the driving force. This means that substrates such as l-lactate migrate from sites of production toward sites of consumption, be it between cells within an organ (e.g., between glia and neurons in the brain or between glycolytic and oxidative fibers in skeletal muscle), or among different organs (e.g., skeletal muscle, heart, and brain) via the blood stream. The equilibrating action of MCTs also provides the basis for lactate acting as a volume transmitter that can mediate metabolic signals through the nervous tissue. ³ The latter concept was underpinned by the demonstration that lactate can bind to the lactate receptor GPR81 (hydroxycarboxylic acid receptor, HCAR1), on brain cells and cerebral blood vessels, resulting in inhibition of adenylyl cyclase. ⁴ The localization and function of HCAR1 and the three MCTs (MCT1, MCT2, and MCT4) expressed in brain will be the focus of this review (Figure 1).

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Figure 1.

Lactate transport and signaling in the brain. (A) Electron micrograph showing double immunogold labeling at synapses between parallel fiber terminals (T) and Purkinje cell spines (S). The section was double-labeled with antibodies to GluR2/3 (15 nm gold particles) and to MCT2 (10 nm gold particles). Note colocalization of MCT2 with GluR2/3 at the synaptic membrane and intracellularly in the spine. Cerebellar cortex, molecular layer. (B) The membranes of the Bergmann glia (A) facing the parallel fiber terminals (T) and Purkinje cell spines (S) are labeled with antibody to MCT4 (10 nm gold particles). (C) The lactate receptor (10 nm gold, red arrowheads) is at the postsynaptic membrane (black arrowheads). Synapse of a nerve terminal (t) on a dendritic spine (s). Stratum radiatum, hippocampus CA1. (D, E) The lactate receptor GPR81/HCAR1 (green) is in the pyramidal cell somatodendritic compartment including spines (white arrows in D), and in microvascular endothelium (white v in E). (D) Magnification of area framed in (E) is shown. Hippocampus CA1. Neurons labeled for microtubule-associated protein 2 (MAP2) (red), nuclei with DAPI (blue). (F) The endfeet (Ae) of the Bergmann glia facing the endothelium (E) strongly express MCT4 (10 nm gold particles). (G) MCT1 (10 nm gold particles) is in the endothelium facing the lumen of the capillary, including in itracellular vesicular organelles (partly indicated by white arrows). (H) Electron micrograph showing GPR81 immunogold particles (red arrowheads) at luminal (short arrow) and abluminal (Abl) membrane of the vascular endothelium and at the perivascular astrocytic end-foot membrane (Ae) at a hippocampal blood vessel. (I) Schematic representation of l-lactate production and action at the ‘tripartite synapse’ of axon, dendrite, and astrocyte, and at the glio-vascular junction (see text). Lactate, formed by glycolysis in brain cells or entering from blood, migrates down concentration gradients (of lactate and cotransported proton) between intracellular and extracellular compartments of neurons, astrocytes, and endothelial cells (orange circle), catalyzed by monocarboxylate transporters (MCT1, MCT2, and MCT4, red ovals). Lactate also migrates along the extracellular space, as well as throughout the astrocytic syncytial network via connexin gap junctions (Cx, orange oval). For simplification, pyruvate and other intermediates are not shown. HCAR1 (red rectangles), lactate receptors, the lactate binding site facing the extracellular space. GLUT1 and GLUT3 (green ovals), glucose transporters, providing glucose for brain cells. Positions of ionotropic glutamate receptors (AMPA-R, purple rectangles) and metabotropic glutamate receptors (blue serpents), influencing and influenced by lactate, are indicated. Mitochondria (dark-blue symbols) shun dendritic spines and thin astroglial processes. Astroglial processes contain glycogen particles (green spheres). (A, B, F, G modified from Bergersen ¹ and C, D, E, H modified from/based on Lauritzen. ⁴ )

Lactate acts as a ‘buffer’ between glycolysis and oxidative metabolism. In this process, it is exchanged as a fuel between cells and tissues with different glycolytic and oxidative rates. ⁵ According to some, lactate is a preferred fuel for brain metabolism. ⁶ At rest, there is a net efflux of lactate from the brain into the blood stream. But when blood lactate levels rise, such as in physical exercise, there is an influx of lactate from blood to brain.^{7, 8} Glucose is the principal energy source of the adult brain. ⁹ The cells can either store glucose, in the form of glycogen, or break it down to pyruvate in glycolysis. The rapid formation of adenosine triphosphate (ATP) through glycolysis may be particularly important after neuronal activation and may explain the immediate increase in glucose uptake with oxygen uptake lagging behind.^{10, 11} However, there is an instantaneous decrease in lactate immediately after neuronal activation, ¹² followed after a few seconds by a rise, indicating that the cells have a latent capacity for lactate oxidation that is rapidly exhausted. The full chemical energy in glucose is released as ATP by metabolism of pyruvate in the mitochondria through the tricarboxylic acid cycle and oxidative phosphorylation. When the rate of glycolysis exceeds that of oxidative phosphorylation, the cells convert pyruvate into lactate via lactate dehydrogenase. The lactate can exit form the cells via MCTs and be metabolized in other cells that also carry MCTs. The cotransport of protons and lactate by MCTs impacts on pH regulation, but is in line with the fact that lactic acid rather than lactate anion is the form produced in glycolysis and consumed in oxidative metabolism and anabolic reactions. ¹³

Monocarboxylate transporters

The MCTs, ² belong to the solute carrier 16 (SLC16) gene family according to the Human Genome Organization (HUGO) nomenclature (www.genenames.org). They are classified as the monocarboxylate porter family (2.A.1.13) according to the Transporter Classification system for functional and phylogenetic classification of membrane transport proteins (http://www.tcdb.org/), approved by the International Union of Biochemistry and Molecular Biology. The MCTs are predicted to have 12 transmembrane domains with the N- and C-termini located intracellularly. Mutagenesis, chimera studies, and molecular modeling have clarified MCT structure and transport mechanism. ¹⁴ Results indicate an uptake cycle in which proton binds before the lactate anion, followed by a domain rearrangement during which lactate and proton pass through the channel between the extracellular and intracellular binding site to get released to the cytosol, lactate first and then proton. A lysine residue (K38), which is thought to bind lactate after getting protonated, at the extracellular substrate binding site and an ion pair formed by aspartate (D302) and arginine (R306) at the intracellular substrate binding site are essential for transport and are not conserved in MCT family members that do not cotransport lactate and proton. The transport cycle can be repeated 12 times per second (MCT1), the rate limiting step being the reversal of the domain rearrangement to return to the extracellularly open conformation. The exchange of intracellular with extracellular monocarboxylate is faster than net transport of monocarboxylate with proton. The most widely used MCT inhibitor has been α-cyano-4-hydroxycinnamate, but this is not specific for MCTs. A novel potent inhibitor (Ki 2 nmol/L for MCT1), AR-C155858 developed by AstraZeneca (Stockholm, Sweden), blocks transport by MCT1 and MCT2, but not by MCT4. ¹⁵ This inhibitor appears to bind from the cytosolic side interacting with the C-terminal part of the protein.

Among the 14 MCT isoforms that have been identified, only MCT1 to MCT4 have been shown to catalyze a proton coupled transport of lactate and other monocarboxylates, including pyruvate and ketone bodies. ¹⁶ MCT1 (2.A.1.13.1, SLC16A1), MCT2 (2.A.1.13.5, SLC16A7), and MCT4 (2.A.1.13.6, SLC16A3) are widely expressed in the brain (see below), whereas MCT3 (2.A.1.13.9, SLC16A8) in the central nervous system is restricted to the basolateral membrane of cells in the retinal pigment epithelium and choroid plexus. ¹⁷ Phylogenetically, as inferred from the sequence, MCT1 to MCT4 are more closely related to each other than to other SLC16 members; MCT2 is closer to MCT1 than to MCT4 and MCT3, ² which correlates with their substrate affinities.

The membrane localization and catalytic function of the transporters depend on ancillary proteins that are members of the immunoglobulin superfamily engaged in cell recognition, and, in contrast to the MCTs, are heavily glycosylated: basigin (CD147, extracellular matrix metalloproteinase inducer, 8.A.23.1.1) forms a complex with MCT1, MCT3, or MCT4, and embigin (gp70, 8.A.23.1.2) interacts with MCT2. ¹⁴ The preference for ancillary protein is not absolute and in rat erythrocytes embigin interacts with MCT1. Interestingly, in human erythrocytes, where basigin is the Ok blood group antigen (and MCT1 is the lactate transporter), basigin is also the receptor for a malaria parasite surface protein and is required for invasion of the erythrocytes by Plasmodium falciparum. ¹⁸ Neuroplastins, other members of the immunoglobulin superfamily adhesion molecules, which regulate the surface expression of glutamate and GABA receptor subunits, have recently been shown to be required for the surface expression of MCT2 and the associated lactate transport, ¹⁹ linking MCT2 with synaptic function (see below under Neurons). Sorting signals in the C-terminal tail direct MCT3 and MCT4 to the basolateral membrane of retinal pigment epithelium cells, whereas MCT1, which lacks such basolateral sorting signal, ²⁰ requires interaction with basigin for targeting to the apical membrane. ²¹

The MCT isoforms have different affinities for lactate and other substrates, partly correlating with their tissue distributions: high-affinity isoforms (MCT1 and MCT2) are expressed in cells that mainly import lactate, the low-affinity isoform (MCT4) in lactate-exporting cells. Consistent with this, MCT4 has a very low affinity for pyruvate, which saves pyruvate for conversion to lactate by lactate dehydrogenase concomitantly converting NADH into NAD⁺, which serves to maintain glycolysis. The rate of transport in either direction depends on the prevailing gradients of substrate and pH, such that net transport is the difference between efflux and influx. At equilibrium, [lactate⁻]_i/[lactate⁻]_o = [H⁺]_o/[H⁺]_i where brackets denote concentration and subscripts i and o denote intracellular and extracellular, respectively.

While MCT mediated net transport always runs in the direction of the combined concentration gradients of monocarboxylate and proton, intracellular lactate is generally higher than extracellular, ²² meaning that a high affinity will favor the capture of extracellular substrate at low concentration, and a low affinity will avoid saturation by intracellular substrate at high concentration.

Whereas a high affinity indicates that the transport will be saturated and independent of the lactate concentration, the surface expression of the transporter is subject to regulation by trafficking between the plasma membrane and intracellular vesicular organelles. Electron microscopy shows such organelles to contain MCT2 in synaptic spines and MCT1 in vascular endothelium^{23, 24} and in cardiac muscle. ²⁵ It has been suggested that signal sequences in the C-terminus may be involved in such translocation targeting. ²

Regulation of MCT expression in relation to activity was shown by neuromuscular trans-reinnervation experiments, ²⁶ regulation in response to pathologic conditions was shown in cardiac ischemia and heart failure models. ²⁵ The MCT protein contents are regulated at the transcriptional level and even more at the translational level. ² The 3′-untranslated region, which is particularly long in MCT1, may be important for translational regulation. Hypoxia causes strong upregulation of MCT4, which is consistent with the presence of potential binding sites for hypoxia inducible factor 1α in the MCT4 promoter but not in the MCT1 and MCT2 promoters. ² Upregulation of the high-capacity, low-affinity MCT4 by hypoxia, recently showed to be mediated transcriptionally by hypoxia inducible factor 1α in cultured astrocytes, ²⁷ would meet the needs of cells to export excess lactate formed in anaerobic glycolysis. Astrocytic expression of MCT4, along with glucose transporter 1 and glycolytic lactate production, is enhanced by hypoxia inducible factor 1α produced in response to nitric oxide from cocultured vascular endothelial cells. ²⁸ In a neural-derived cell line, MCT1 as well as MCT4 were found to be upregulated in hypoxia, whereas MCT2 was downregulated. ²⁹ As MCT2 has a particularly high affinity for pyruvate, downregulation of MCT2 would limit the loss of pyruvate from the cell, saving it for conversion to lactate to regenerate NAD⁺ from NADH. AMP-activated protein kinase, which guards against deficient ATP regeneration by upregulating catabolic pathways including glycolysis, ³⁰ likely contributes to the upregulation of MCTs in response to hypoxia and increased activity-dependent energy demand in the brain, such as observed for MCT1 and MCT4 in heart and skeletal muscle on chronic stimulation or exercise. ³¹ The transcriptional coactivator PGC-1α (peroxisome proliferator-activated receptor-γ (PPARγ) coactivator-1α), a master regulator of reactive oxygen species scavenging enzymes and of mitochondrial biogenesis in brain, ³² may also participate. Thus, PGC-1α ³³ is known to upregulate MCTs in other tissues, and so does PPARα, ³⁴ which mediates adaptive responses to energy restriction and induces the formation of ketone bodies. Food deprivation has been shown to upregulate MCT2, but not MCT1, in a brain stem nucleus ³⁵ (the area postrema—solitary tract nucleus).

Brain-derived neurotrophic factor (BDNF) upregulates MCT2, but not MCT1 or MCT4, through several signaling mechanisms involved in synaptic plasticity, including mitogen-activated protein kinase, and mammalian target of rapamycin, in cultured mouse cortical neurons ³⁶ and in hippocampus in vivo. ³⁷ Noradrenalin ³⁸ as well as insulin and insulin-like growth factor ³⁹ also cause MCT2 upregulation through mitogen-activated protein kinase and mammalian target of rapamycin. The upregulation of MCT2 by these mechanisms is in line with its enrichment at glutamatergic synapses and its involvement in neuronal activity, synaptic plasticity, and memory formation (see below under Neurons).

In the brain, the low-affinity and high-capacity transporter MCT4 is on astrocytes (Figures 1B, 1F, and 1I),^{23, 40} cells that are glycolytic and can supply lactate to other brain cells. Due to the high Km (about 30 mmol/L), MCT4 will usually not be saturated, and the transport rate will therefore increase with the lactate concentration. MCT2 is found on the cell bodies, dendrites, dendritic spines (Figures 1A and 1I), and axons of neurons,^{23, 24, 41} cells that are highly oxidative and are likely to mainly import lactate.^{42, 43, 44} The Km of less than 1 mmol/L ⁴⁵ suggests that MCT2 will be saturated with lactate in most conditions, and that efflux as well as uptake rates will be largely independent of concentration. MCT1 is mainly detected on endothelial cell of the blood—brain barrier (BBB) (Figures 1G and 1I), both in rodents^{23, 46, 47, 48} and in humans.^{49, 50} By being expressed on cerebral microvessels, MCT1 is part of the physiologic barrier that controls the passage of these molecules between the blood and the brain, for example, during exercise when blood lactate is high. ⁵¹ MCT1 has an intermediate-to-high affinity for lactate (about 4 mmol/L). ⁴⁵ However, Lee et al ⁵² found no MCT1 in endothelial cells. Still more controversial is the presence of MCT1 on mitochondria ⁵ which is challenged by others and is difficult to reconcile with the generally large difference in NADH/NAD⁺ ratios between the cytosol and the mitochondria. ² Recently, MCT1 was found on oligodendrocytes. ⁵³ This isoform is expressed even on the myelin sheath.^{52, 53} MCT3 has an intermediate affinity (Km 6 mmol/L) ⁵⁴ and is predominantly expressed in retinal epithelial cells and in the choroid plexus epithelial cells (which are developmentally homologous to the latter), but not in the ependymal cells lining the cerebral ventricles. ¹⁷

The high-affinity transporter MCT2 is abundantly detected on neurons and astrocytes by immunohistochemical methods, ⁵⁵ but shows substantial species differences in distribution. In rat brain, MCT2 immunoreactivity has primarily been found on perivascular endfeet^{8, 9, 56} and postsynaptic densities.^{23, 24} MCT4 is believed to be mainly localized on astrocytes, both on plasma membranes of perisynaptic processes and on perivascular endfeet.^{23, 40}

In situ hybridization pictures (http://mouse.brain-map.org/experiment/show?id=72001956, The Allen Institute for Brain Science) are consistent with the immunocytochemical observations. MCT2 (Slc16a7) pictures indicate a general distribution of mRNA in neurons of the mouse brain, including in pyramidal cells in the hippocampal and cerebral cortex, as well as in blood vessels. MCT4 (Slc16a3) pictures (http://mouse.brainmap.org/experiment/show?id=69838404) show a less clear signal, compatible with localization in the astrocytic network. MCT1 (Slc16a1) pictures indicate vascular localization (http://mouse.brain-map.org/experiment/show?id=68203467).

Lactate receptor GPR81/HCAR1

In addition to its role as a metabolite and energy substrate, lactate is a signaling substance. It may signal in several ways, thus lactate modifies prostaglandin action contributing to vasomotor regulation, ⁵⁷ and influences the NADH/NAD⁺ ratio contributing to redox regulation. ⁵ Lactate signaling through a specific receptor protein, HCAR1, was shown in the brain only in 2013, ⁴ and the distribution of the protein (Figures 1C, 1D, 1E, 1H, and 1I) was delineated the year before. ⁵⁸ In a review, published in July 2013, ¹² the possibility that HCAR1 mediates lactate action in brain was mentioned as an outstanding question—‘Functional demonstration of HCAR1 in brain may reveal a whole new dimension to metabolic signaling in this organ’. Subsequently, HCAR1 was shown to modify electrical activity in primary neuronal cultures. ⁵⁹ More recently, action of lactate through a yet unidentified receptor was reported in locus coeruleus ⁶⁰ (without reference to the previous work). Lactate is the endogenous ligand for the HCAR1^{61, 62} previously classified as an orphan receptor. Lactate activates HCAR1 within the physiologic concentration range (1 to 20 mmol/L) of lactate. Computational modeling and mutation analysis have shown that Arg71, Arg99, Glu166, and Arg240 of the human HCAR1 are important sites for lactate interaction. ⁶³

GPR81, a G-protein-coupled orphan receptor was previously discovered to be selectively activated by lactate, downregulating cAMP through G_i action to inhibit lipolysis in adipocytes.^{61, 62, 64} GPR81 is therefore subsequently named hydroxycarboxylic acid receptor 1 (HCA₁, IUPHAR nomenclature; HCAR1, HUGO nomenclature), and has cogeners, HCAR2 and HCAR3, that respond to other metabolites than lactate.^{65, 66} The HCARs are categorized as the G-protein coupled receptor family, Class A (rhodopsin-type), 7: Hydroxy-carboxylic acid receptors (http://www.genenames.org/genefamilies/GPCR). The HCAR genes are all located on the short arm of human chromosome 12 (12q24.31), where also the gene of the neuronal MCT (MCT2) is located (12q.13).

In adipose tissue, where HCAR1 mRNA is expressed at levels that by far exceed those in other tissues, the lactate receptor appears to serve a special role, as it does not respond primarily to increases in ambient lactate supplied via the circulation, but rather to locally high-lactate concentrations released from adipocytes as an autocrine produced in response to insulin-dependent uptake of glucose. ⁶⁴ Could GRP81 signaling occur in the brain?

Lactate in the physiologic concentration range was shown to downregulate forskolin-stimulated cAMP levels in hippocampal slices. ⁴ 3,5-Dihydroxy benzoic acid (3,5-DHBA), which stimulates HCAR1 but not the cogeners HCAR2 and HCAR3 ⁶⁷ reproduced the effects of lactate, with concentration dependency similar to that in adipocytes. The results indicate that the lactate receptor is present and active in brain at levels high enough to regulate cAMP-dependent functions. Immunofluorescence showed that HCAR1 is highly concentrated in the principal neurons, such as hippocampal pyramidal cells and Purkinje cells, as well as in interneurons in multiple brain regions including the hippocampus and the cerebellum. In the principal cells, it is concentrated in the somatodendritic compartment, particularly in the spines. Astroglia and vascular endothelium showed some labeling. Electron microscopic immunogold quantification showed the highest immunoreactivity at the postsynaptic membrane of excitatory type synapses on dendritic spines of pyramidal cells, but also a signal in vascular endothelial cells, i.e., at the BBB, and in perivascular and perisynaptic processes of astrocytes. ⁴

The action of lactate described in locus coeruleus ⁶⁰ increases rather than suppresses cAMP levels and in contrast to HCAR1 is selective for l-lactate versus d-lactate. The authors therefore dismissed the option that the effects were mediated by HCAR1 and proposed that a yet unidentified receptor is involved. However, G-protein-coupled receptors are known to signal through varying coupling mechanisms showing bias for different mechanisms among different ligands, ⁶⁸ which implies the possibility that HCAR1 could cause the observed effects.

While rtPCR indicated HCAR1 mRNA to be an order of magnitude less abundant in hippocampus than in adipose tissue (per protein content), ⁴ in situ hybridization has shown a distribution of HCAR1 mRNA throughout the brain, including in principal neurons of the cerebral, hippocampal, and cerebellar cortices (The Allen Institute for Brain Science, http://www.brain-map.org; GENSTAT, http://www.ncbi.nlm.nih.gov/gensat; St Jude Children's Research Hospital, http://www.stjudebgem.org). These observations indicate functions of the lactate receptor in widespread regions of the central nervous system, mediating effects of lactate that may serve to link synaptic function, energy metabolism, and cerebral blood flow.

Neurons

The main MCT expressed in neurons is MCT2 (Figures 1A and 1I). ¹ Early work reported colocalization of MCT2 with microtubule associated protein 2. ⁶⁹ Notably, MCT2 is concentrated at the postsynaptic membranes of glutamatergic synapses on spines of Purkinje cells in cerebellum ²³ and pyramidal cells in the hippocampus. ²⁴ The density is lower at mossy fiber synapses, which have lower prevalent firing rate, compared with Schaffer-collateral synapses. The lack of MCT2 at inhibitory type synapses on pyramidal cell somata suggests a connection to glutamatergic neurotransmission. In agreement with this notion, quantitative immunogold double-labeling (Figure 1A) showed that MCT2 is colocalized with subunits GluR2/3 (GluA2/3) of the AMPA type glutamate receptor. ²⁴ This receptor is known to undergo trafficking between the plasma membrane and intracellular vesicular stores in the spines as part of activity-dependent synaptic plasticity. ⁷⁰

HCAR1, like MCT2, shows the highest concentration in neurons, including pyramidal cells (Figures 1D, 1E, and 1I). HCAR1^{4, 58} as well as MCT2 ²⁴ show a dual localization on the synaptic membrane (Figures 1C and 1I) and intravesicular organelles, probably reflecting protein trafficking. The presence of the lactate receptor along with a lactate transporter on intracellular vesicular organelles brings up the interesting possibility that intravesicular lactate could contribute to lactate signaling, which would be a novel principle. In any case, the main finding of the two proteins at postsynaptic membranes of glutamatergic synapses, together with glutamate receptors, suggests an intimate relation of lactate to glutamatergic neurotransmission.

Such a relation has previously been advanced on purely metabolic grounds as part of the astrocyte-to-neuron lactate shuttle (ANLS) hypothesis, according to which supply of lactate from astrocytes, driven by glycolysis due to astrocytic uptake of transmitter glutamate released from neurons, ⁴³ is essential for neuronal functions such as memory formation. ⁷¹ Although the ANLS is disputed, ²² the role of lactate in synaptic plasticity is consistent with the observation that MCT2 expression is enhanced, together with postsynaptic density 95 protein and glutamate receptor subunit 2 (GluR2) protein, by BDNF, ³⁷ a central mediator of synaptic plasticity. However, the direction of flux through this MCT2 is not known. Lactate, whether from blood or produced in astrocytes, may enter dendritic spines through the MCT2 in the postsynaptic membrane to assist aerobic energy production. This would be in line with the notion that postsynaptic ion currents consume the major part of the energy production in the brain. ⁷² However, while mitochondria are essential for spine development and function, the synaptic spines themselves rarely have mitochondria ⁷³ meaning that the lactate would have to diffuse into the dendrite branch before being oxidized to yield ATP. In line with this, the initial response to neuronal activation appears to be glycolysis for fast ATP production, ¹¹ rather than glucose oxidation. An obvious reason for this is that cytosolic glycolysis takes place close to the sites of ATP consumption while mitochondria are at a distance. In agreement, there is evidence that the glycolytic complex of enzymes is associated with plasma membranes as well as with endoplasmic reticulum membranes, and that regulation and function of ion channels and transporters, including the ATPases that pump K⁺, Na⁺ and Ca²⁺, depend on ATP generated by glycolysis. ¹³ Lactate produced in the spine may either diffuse to reach mitochondria in the dendrite or exit the spine through the MCT2 of the synaptic membrane for further metabolism elsewhere, including reentering the neuron later, when the intraneuronal lactate concentration decreases. On activation of AMPA receptors by climbing fiber stimulation, cerebellar Purkinje cells produce lactate from glucose in the absence of glycogenolysis; blocking AMPA receptors by CNQX prevented the rises in extracellular lactate, postsynaptic currents, blood flow, and glucose and oxygen consumption. ⁷⁴ These observations indicate that the extracellular lactate produced on neuronal stimulation exits rather than enters the neurons (Figure 1I). A similar conclusion was arrived at based on computer modulations. ⁷⁵

The lactate leaving neurons immediately after activation may be dispersed in the extracellular space or through the astrocytic, gap-junction connected syncytial network to be flushed out into cerebrospinal fluid, blood, or lymph where the lactate concentration is low at physical rest. It may also be taken up and oxidized in neighboring neurons. The total contribution of the ANLS may account for some 10% of total glucose uptake and oxidation in brain. ⁷⁶ An interesting thought is that mobilization of astrocyte glycogen during brain activation may reduce utilization of blood-borne glucose by astrocytes because of hexokinase inhibition, increasing glucose availability for activated neurons. ⁷⁷ Regardless of the quantitative energetic importance of the ANLS, the lactate generated on neuronal activation could exert functions, perhaps partly through HCAR1, that may contribute to observations by the Magistretti group.^{71, 78}

An additional rationale for having an MCT at the spine membrane would be to limit acidification, which is known to adversely affect glutamate receptors. Thus, NMDA receptors and kainate receptors are sensitive to pH.^{79, 80} In addition, intracellular acidification may regulate excitability through modifying chloride channels, as observed in skeletal muscle. ⁸¹ We suggest that lactate produced through activity initiated glycolysis in the spine exits the spine through the synaptic MCT2 to activate the colocalized lactate receptor, acting as an autocrine to confine the rate of spine glycolysis.

Astrocytes

The low-affinity transporter MCT4 appears to be the main MCT in astrocytes (Figures 1B, 1F, and 1I),^{23, 40} at least in rodents. As low affinity is considered suitable for efflux rather than influx (see above), the expression of MCT4 in astrocytes is in line with the notion of the astrocyte as mainly glycolytic and the fact that it is the only brain cell containing appreciable quantities of glycogen. ⁷³ MCT1 was initially reported in cultured astrocytes ⁴⁵ and MCT2 ⁵⁰ has also been reported to be expressed to some extent in astrocytes. The discrepancies in localization may result from differences among species and in experimental conditions. MCT4 appears to be restricted to astrocytes throughout development and reaches adult levels 14 days postnatally, ⁴⁰ i.e., proceeding the time of synaptogenesis. ⁸² MCT4 is primarily located on the plasma membrane, on perisynaptic as well as perivascular processes of the astrocyte, which means that the astrocyte forms a direct channel for lactate between the blood vessels and the computing machinery of the brain. As expected, because the total area of the perisynaptic membranes is larger than that of the juxtavascular area, the density of MCT4 is considerably higher on the juxtavascular face of the perivascular endfeet compared with other parts of the astrocyte.

HCAR1 is relatively sparsely expressed in astrocytes in the brain, but occurs at astrocytic endfeet (Figures 1H and 1I). ⁴

Oligodendrocytes

Lactate is taken up and used by developing oligodendrocytes in primary culture ⁸³ and organotypic brain slices. ⁵³ During development, the need for substrates to support lipid synthesis is highly increased: at the peak of myelination, oligodendrocytes have been estimated to synthesize an amount of lipids equal to three times their cell body weight daily. ⁸⁴ The acetylation of histones, which is important for oligodendrocyte differentiation and for myelination, also requires carbon substrates. ⁸⁵ The myelinating oligodendrocytes and their precursor cells are particularly vulnerable to energy deprivation, compared with neurons and other brain cells. ⁸⁶ Energy deprivation leads to a reversal of glutamate transporters, which increases the extracellular glutamate concentration. Energy deprivation also triggers activation of AMPA/kainate receptors and NMDA receptors expressed on oligodendrocyte lineage cells causing damage through increased intracellular Ca²⁺.^{87, 88, 89, 90} Glutamate receptors are also expressed in axons,^{91, 92} which are damaged by the same mechanisms. Lack of energy inhibits myelination by limiting the accessibility of carbon substrates for the synthesis of myelin lipids. ⁵³ In this way, oligodendrocytes require alternative substrates such as lactate for survival and to satisfy their biosynthetic demand. Similarly, organotypic brain slices cultured in low-glucose media showed loss of oligodendrocytes and lack of myelination, which could be avoided by supplying lactate.^{53, 93} Besides lactate, ketone bodies are present at high concentrations in the blood at the time of myelination in humans and rats ⁹⁴ and are important for myelination ⁸⁴ and hence for sustaining long axons.

After myelination has taken place, the use of lactate by oligodendrocytes apparently changes. In adult mice, the myelin sheath was found to release lactate that was used by axons.^{52, 93, 95} This is in line with the notion that long axons are supported by lactate from glycolysis in oligodendrocytes. ⁹⁶ The results suggest that oligodendrocytes may depend on import of lactate for ATP production and lipid synthesis during development, followed by a switch to other metabolic pathways and lactate export after myelination comes to a steady state. Support for this developmental switch was obtained using mutant mice in which oxidative phosphorylation was disabled in the oligodendrocytes. The findings suggested that mature myelinating oligodendrocytes do not need oxidative phosphorylation and can rely solely on glycolysis for ATP production. Knockdown of MCT1 expression specifically in oligodendrocytes established that lactate released from the oligodendroglial compartment is crucial for axon health. ⁵² The MCT1 knockdown led to abnormal axon morphology and neuronal death in organotypic cultures that could be rescued by supplying lactate. ⁵² MCT1 knockdown caused pathology similar to that seen in patients with amyotrophic lateral sclerosis (ALS). In addition, ALS patients and SOD1 mutant mice (a mouse model for ALS) show reduced levels of MCT1 in oligodendrocytes. Failure in the supply of lactate from oligodendrocytes (or disruptions upstream of this) could therefore be involved in the pathogenesis of ALS and other neurodegenerative diseases.

Roles of lactate in oligodendrocytes and myelinated axons have been reviewed recently. ⁹³

The blood—brain barrier

MCT1 is the MCT expressed at the BBB, constituted by the endothelial cells of cerebral blood vessels (Figures 1G and 1I). This barrier represents the body—brain interface, the gateway to the brain. The transporter is localized at similar densities at the luminal and abluminal surfaces of the endothelium, as well as over cytoplasm, presumably on vesicular organelles.^{1, 23} This intracellular pool of MCT1 may constitute a reservoir for regulating the amount of transporter at the plasmalemma according to need.

A similar dual plasmalemmal and intracellular localization was observed for the lactate receptor HCAR1 in brain capillary endothelium, which showed receptor levels intermediate between those in neurons and in astrocytes (Figures 1H and 1I). ⁴

Lactate—A volume transmitter

A ‘volume transmitter’, as opposed to a ‘wiring transmitter’ at synapses, reaches receptors distributed in a considerable volume surrounding the site of release. ⁹⁷ The existence of a lactate receptor in brain ⁴ bolsters the role of lactate as a ‘volume transmitter’, a role suggested in a perspectives article in 2012. ³ The distribution of MCTs among brain cells (Figure 1) sets the scene for this volume transmitter action of lactate: through the equilibrating, facilitatory transport mediated by the MCTs, lactate will diffuse down its concentration gradient from sites of net production to reach lactate receptor sites via intracellular and extracellular compartments. The entry of lactate into brain tissue at the BBB to act on HCAR1 on brain cells is related to the volume transmission concept.

The activation of the lactate receptor to lower cAMP levels may serve to regulate multiple cellular processes, including curbing glycolysis and glycogenolysis to save energy and limit acidification when glucose breakdown is faster than oxidation through mitochondria. This may counteract damage in hypoxic conditions, but may even have a role in the first moments of cell activation, when glucose uptake and its conversion to lactate increase earlier than oxygen uptake.^{11, 75} As dealt with further below, another beneficial effect would be to limit the activity of cAMP activated potassium channels and thereby counteract excessive hyperpolarization. In addition, HCAR1 may have noncanonical activities, such as through β-arrestin. The downstream mechanisms and the physiologic importance of these and other effects of stimulating the lactate receptor remain to be explored.

Besides acting through the lactate receptor, we suggested ³ that the volume transmitter action of lactate could take effect through modification of the redox state of cells. When lactate equilibrates over cellular membranes, the redox potential changes, and could act as a ‘redox switch mechanism’.^{98, 99} Numerous cellular processes are subjected to redox regulation, notably through modified gene expression by histone deacetylases. ¹⁰⁰ Interestingly, redox modulation of the NMDA receptor has recently been shown to mediate several of the effects of lactate on brain function. ¹⁰¹

Targets for new therapeutic drugs and interventions