Fifty male and 49 female B6;129 mice (wild-type, + / + ) were maintained until 2 years of age to study their age-related pathology. By 104—105 weeks, 14/50 (28%) of the males and 30/49 (61%) of the females were still alive. The most common contributing cause of morbidity or mortality was lymphoma. Lymphoma was observed in 21/50 (42%) of the males and 33/49 (67%) of the females with the most common sites being mesenteric lymph nodes, gut associated lymphoid tissue (Peyer's patches), and spleen. The lymphoma most often appeared to arise in the mesenteric node. Immunohistochemistry revealed CD45R expression as well as infiltration by many CD3+ T cells. IgH gene rearrangements were found in typical mesenteric node lymphomas indicating B-cell origin. They bore similarities to the human T-cell rich, B-cell lymphomas. Other tumors included hepatocellular adenoma or carcinoma (male 12%, females 10%), lung alveolar Type II cell adenoma or carcinoma (male 32%, female 20%), thyroid follicular adenoma or carcinoma (male 2%, female 8%), ovarian tumors (17%), and endometrial tumors (6%). Nonneoplastic lesions included amyloidlike material in the nasal septum (male and female 100%), otitis media (male 84%, female 79%), epididymal epithelial karyomegaly (88%), melanosis (high incidences in various tissues including brain, parathyroid, and spleen), membranoproliferative glomerulonephritis (male 52%, female 71%), hyalinosis with extracellular crystals in several tissues (respiratory tract, gall bladder, stomach), islet cell hyperplasia (male 45%, female 29%) and esophageal dilation (male 10%, female 6%). The B6;129 mouse is a mouse with aging lesions similar to those in other mouse strains but with a characteristic common lymphoma.
Blackwell BN , Bucci TJ, Hart RW, Turturro A. (1995). Longevity, body weight, and neoplasia in ad libitum-fed and diet-restricted C57BL6 mice fed NIH-31 open formula diet. Toxicol Pathol23: 570—582.
2.
Boorman GA, Rafferty CN, Ward JM, Wills RC (2000). Leukemia and lymphoma incidence in rodents exposed to low-frequency magnetic fi elds. Rad Res153: 627—636.
3.
Donehower LA , Harvey M., Vogel H., McArthur MJ, Montgomery CA, Park SH, Thompson T., Ford RJ, Bradley A. (1995). Effects of genetic backgroun d on tumorigenesis in p53-defi cient mice. Mol Carcinog14: 16—22.
4.
Eckhaus MA, Kopp JB ( 2000). Renal diseases in genetically engineered mice. In: Pathology of Genetically Engineered Mice, Ward JM, Mahler JF, Maronpot RR, Sundberg JP (eds). Iowa State University Press, Ames, Iowa, pp 347—364.
5.
Frith CH, Ward JM, Frederickson T., Harleman JH (1996). Neoplastic lesions of the hematopoieti c system . In: Pathobiology of The Aging Mouse, Mohr U, Dungworth DL, Capen CC, Carlton WW, Sundberg JP, Ward JM (eds). ILSI Press, Washington, DC, pp 219—235.
6.
Gross-Bellard M., Oudet P., Chambon P. (1973). Isolation of high molecular weight DNA from mammalian cells. Eur J Biochem36: 32—37.
7.
Harris NL, Jaffe ES, Stein H., Banks PM, Chan JKC, Cleary ML, Delsol G., De Wolf-Peeters C., Falini B., Gatter KC, Grogan TM, Isaacson PG, Knowles DM, Mason DY, Muller-Hermelink H., Pileri SA, Piris MA, Ralfkiaer E., Warnke RA (1994). A revised European-American classfi cation of lymphoid neoplasms: a proposal from the International Lymphoma Study Group . Blood84: 1361—1392.
8.
Hartley JW, Chattopadhyay SK, Lander MR, Taddesse-HeathL., Naghashfar Z., Morse HC, Fredrickson TN (2000). Accelerated appearance of multiple B cell lymphoma types in NFS/N mice congenic for ecotropic murine leukemia viruses. Lab Invest80: 159—169.
9.
Hendrick SM, Germain RN, Bevan MJ, Dorf M., Engel I., Fink P., Gascoigne N., Heber-Katz E., Kapp J., Kaufman Y., Kaye J., Melchers F., Pierce C., Schwartz R., Sorensen C., Taniguchi M., Davis MM (1985). Rearrangement and transcription of a T-cell receptor β-chain gene in different T cell subsets. Proc Natl Acad Sci USA82: 531—535.
10.
Herbert RA, Leininger JR (1999). Nose, larynx and trachea. In: Pathology of the Mouse, Maronpot RR (ed). Cache River Press, Vienna, IL, pp 259— 292.
11.
Hursting SD , Perkins SN, Brown CC, Haines DC, Phang JM (1997). Calorie restriction induces a p53-independen t delay of spontaneou s carcinogenesis in p53-defi cient and wild-type mice. Cancer Res57: 2843—2846.
12.
Johnson M., Leonard G., Kreutzer DL (1997). Murine model of interleukin-8-induced otitis media . Laryngoscope107: 1405—1408.
13.
Kelemen G. (1978). Diseases of the ear. In: Pathology of Laboratory Animals, Vol. I, Benirschke K, Garner FM, Jones TC (eds). Springer-Verlag, New York, pp 620—629.
14.
Kodell RL, Blackwell BN, Bucci TJ, Greenman DL (1995). Cause-of-death assignment at the National Center for Toxicological Research. Toxicol Pathol23: 241—247.
15.
Lang RB, Stanton LW, Marcu KB (1982). On immunoglobulin heavy chain switching: two γ 2b genes are rearranged via switch sequences in MPC-11 cells but only one is expressed. Nuclei Acid Res10: 611—630.
16.
Li S., Wang Q., Chakladar A., Bronson RT, Bernards A. (2000). Gastric hyperplasia in mice lacking the putative Cdc42 effector IQGAP1. Mol Cell Biol20: 697—701.
17.
Losco PE (1995). Dental dysplasia in rats and mice. Toxicol Pathol23: 677—688.
18.
Mahler M., Rozell B., Mahler JF, Merlino G., Devor-Hennema n D., Ward JM, Sundberg JP (2000). Pathology of the gastrointestinal tract in genetically engineered mice. In: Pathology of Genetically Engineered Mice , Ward JM, Mahler JF, Maronpot RR, Sundberg, JP (eds). Iowa State University Press, Ames, Iowa, pp 269—297.
19.
Maronpot, RR, Boorman GA, Gaul BW ( 1999). Pathology of the Mouse: Reference and Atlas. Cache River Press, Vienna, IL, p. 699.
20.
McClatchey AI, Saotome I., Mercer K., Crowley D., Gusella JF, Bronson RT, Jacks T. (1998). Mice heterozygous for a mutation at the Nf2 tumor suppressor locus develop a range of highly metastatic tumors. Genes Dev12: 1121—1133.
21.
McGinn MD, Bean-Knudsen D., Ermel RW (1992). Incidence of otitis media in CBA/J and CBA/CaJ mice. Hear Res59: 1—6.
22.
Meek RB, McGrew BM, Cuff CF, Berrebi AS, Spirou GA, Wetmore SJ (1999). Immunologic and histologic observations in reovirus-induced otitis media in the mouse. Ann Otol Rhinol Laryngol108: 31—38.
23.
Mitchell CR , Kempton JB, Scott-Tyler B., Trune DR (1997). Otitis media incidence and impact on the auditory brain stem response in lipopolysaccharide-nonresponsiv e C3H/HeJ mice. Otolaryngol Head Neck Surg117: 459—464.
24.
Mohr U., Dungworth DL, Capen CC, Carlton WW, Sundberg JP, Ward JM ( 1996). Pathobiology of the Aging Mouse, Volumes 1 and 2. ILSI Press , Washington DC, pp 527, 505.
25.
Perkins SN, Hursting SD, Phang JM, Haines DC (1998). Calorie restriction reduces ulcerative dermititis and infection-related mortality in p53-defi cient and wild-type mice. J Invest Dermatol111: 292—296.
Porter DD, Porter HG, Cox NA (1973). Immune complex glomerulonephri- tis in one-year old C57BL/6 mice induced by endogenou s murine leukemia virus and erythrocyte antigens. J Immunol111: 1626—1633.
28.
Sheldon WG, Bucci TJ, Blackwell B., Turturro A. (1996). Effect of ad libitum feeding and 40% feed restriction on body weight, longevity, and neoplasms in B6C3F1, C57BL6, and B6D2F1 mice . In: Pathobiology of the Aging Mouse, Vol. 2, Mohr U, Dungworth DL, Capen CC , Carlton WW, Sundberg JP, Ward JM (eds). ILSI Press, Washington, DC, pp 21—26.
29.
Sundberg JP, King LE Jr (2000). Skin and its appendages: normal anatomy and pathology of spontaneous, transgenic, and targeted mouse mutations. In: Pathology of Genetically Engineered Mice, Ward JM, Mahler JF, Maronpot RR, Sundberg JP (eds). Iowa State University Press, Ames, Iowa, pp 183— 215.
30.
Taddesse-Heath L., Morse HC III (2000). Lymphomas in genetically engineered mice. In: Pathology of Genetically Engineered Mice , Ward JM, Mahler JF, Maronpot RR , Sundberg, JP (eds). Iowa State University Press, Ames, Iowa, pp 365—381.
31.
Tarone RE, Chu KC, Ward JM ( 1980). Variability in the rates of some naturally occurring tumors in F344 rats and B6C3F1 mice. J Nat Cancer Inst66: 1175—1181.
32.
The Jackson Laboratory ( 2000 ). Jax Bulletin No. 4, http://jaxmice.jax.org/html/nomenclature/hybrid.pdf.
33.
Turusov V, Mohr U (eds) (1994). Pathology of Tumours in Laboratory Animals, Volume II—Tumours of the Mouse. Lyon, IARC Scientific Publications No. 111, pp 776.
34.
Ward JM, Mahler JF, Maronpot RR, Sundberg, JP (2000). Pathology of Genetically Engineered Mice. Iowa State University Press, Ames, Iowa , p 394.
35.
Ward JM, Anver MR, Mahler JF, Devor-Henneman DE (2000). Pathology of mice commonly used in genetic engineering (C57BL/6, 129, B6,129 and FVB/N). In: Pathology of Genetically Engineered Mice, Ward JM, Mahler JF, Maronpot RR, Sundberg JP (eds). Iowa State University Press, Ames, Iowa, pp 161—179.
36.
Ward JM, Taddesse-Heath L., Perkins SN, Chattopadhyay SK, Hursting SD, Morse HC III (1999). Splenic marginal zone B-cell and thymic T-cell lymphomas in p53-defi cient mice. Lab Invest79: 3—14.
37.
Ward JM, Mann PC, Morishima H., Frith CH (1999). Thymus, spleen and lymph nodes. In: Pathology of the Mouse, Maronpot RR (ed). Cache River Press, Vienna, IL, pp 333—360.
38.
Ward JM, Yoon M., Anver MR, Haines DC, Kudo G., Gonzalez FJ, Kimura S. (2001). Hyalinosis and Ym1/Ym2 gene expression in the stomach and respiratory tract of 129S4/SvJae and wild-type and CYP1A2-Null B6,129 mice. Am J Pathol158: 323—332.
39.
Wright JA, Horne M., Stewart MG (1991). An immunohistochemica l study of spontaneous lymphomas in the C57B1/10J mouse. J Comp Pathol104: 211—222.
