P01.22 LB
Purpose: To investigate the effects of compound Qin Qing liquid on AMPK (AMP-activated proteinkinase, AMPK) protein expression levels in rat kidney tissue of high uric acid kidney injury, and study in molecular mechanisms of compound Qin Qing liquid in inhibiting high uric acid kidney injury.
Methods: SD rats were randomly divided into control group, model group, positive group, and high-, medium-, low-dose groups of Chinese medicine. The molding method was gavaging adenine and feeding yeast. Control group and model group were daily gavaged with distilled water (10ml/kg · d), positive control group was daily gavaged with allopurinol by 9.33 mg/kg. High-, medium-, low-dose group of Chinese medicine was daily gavaged with Compound Qin Qing Ligui by 3.77/(kg · d), 1.89/(kg · d), 0.09 g/(kg · d), ig 6 weeks. Some rats of each group were sacrificed at the 4th and 6th week, and their kidney were taken for observing the protein expression of AMPKα1 and AMPKα2 by western-blot and immunohistochemical staining.
Results: Protein levels of AMPKα1 and AMPKα2 was significantly up-regulated by compound Qin Qing Liquids.
Conclusion: The main molecular mechanisms of compound Qin Qing liquid inhibited immune inflammatory injury in high uric acid kidney tissue may be associated with the activation and increases expression of AMPK.
Contact: Fengxian Meng, liuo9@sina.com
