P05.01
Purpose: Development of a simple, sensitive and stability indicating TLC-densitometric method for quantification of naringin in the methanol extracts of stems and leaves of Rumex vesicarius.
Methods: Chromatography was performed on glass-backed silica gel 60 F254 HPTLC plates with the green solvents Ethyl acetate: GAA: MeOH: H2O in proportion of 30:10:5:1, v/v/v/v as mobile phase. Scanning and quantification of developed plate was done densitometrically at 275 nm. Naringin was subjected to acid and alkali hydrolysis, peroxide treatment, photodegradation, dry heat, moist heat and UV treatment for the stability studies.
Results: The system was found to give compact spot for naringin at Rf=0.46±0.001. The linear regression analysis data for the calibration plots showed good linear relationship with r=0.9973 with respect to area in the concentration range of 100–1000 ng. The regration equation of standard was found to be Y=8.448X+21.395. The method was validated for detection and quantification limits, precision, recovery and robustness. The LOD and LOQ were found to be 8 and 24 ng band-1, respectively. The drug undergoes complete degradation under acidic treatment and mild degradation under basic and H2O2 treatment. The degraded products were well separated from the pure drug. The statistical analysis proves that the developed method for quantification of Naringin is reproducible and selective. The content of Naringin in the stems and leaves of R. vesicarius were found to be 1.35% and 2.73% w/w, respectively.
Conclusion: Due to the ability of this method in separating degraded products from the pure drug (naringin), it can be employed as stability-indicating method for in process as well as finished products in the market.
Contact: Mohammed Fahad Alajmi, alajmister@gmail.com
