Antiviral Activity of HIV Type 1 Protease Inhibitors Nelfinavir and Indinavir in Vivo Is Not Influenced by P-Glycoprotein Activity on CD4 + T Cells

P-glycoprotein (P-gp) can compromise the antiretroviral effect of a protease inhibitor (PI)-containing regimen for HIV-1, but can also reduce HIV-1 replication. We studied the net effect of P-gp on the intracellular HIV-1 RNA and DNA load in vivo. CD4⁺ T cells were isolated from 27 HIV-1 patients (13 without and 14 with a PI-containing regimen) and subsequently sorted in CD45RO⁻ (naive) and CD45RO⁺ (memory) subsets with either high (P-gp^high) or low (P-gp^low) P-gp activity. Unspliced HIV-1 RNA and HIV-1 DNA load were determined. For each patient P-gp^high and P-gp^low subsets were compared. In patients on a PI-containing regimen, intracellular unspliced HIV-1 RNA was significantly lower in P-gp^high-naive CD4⁺ cells compared to P-gp^low-naive CD4⁺ cells (p = 0.04). The same trend was seen in naive CD4⁺ cells of treatment-naive patients. In both treated and untreated patients HIV-1 DNA levels were significantly lower in P-gp^high than in P-gp^low memory CD4⁺ cells (p = 0.02 and p = 0.04). High cellular P-gp activity coincided with a reduced intracellular HIV-1 load in vivo, both in therapy-naive and in PI-treated patients. Therefore we conclude that the potential efflux function of P-gp on PIs may be clinically less relevant than the effect of P-gp on intracellular HIV-1 replication.