New Insights into the Regulation of Neutrophil NADPH Oxidase Activity in the Phagosome: A Focus on the Role of Lipid and Ca 2+ Signaling

Importance of cytochrome b₅₅₈ phosphorylation

The phosphorylation of cytochrome b ₅₅₈ in neutrophils is poorly documented, although the phosphorylation of the p22^phox subunit has been observed a long time ago (40). Later, McPhail and coworkers demonstrated that p22^phox phosphorylation is correlated with NADPH oxidase activity, suggesting that the phosphorylation of p22^phox regulates multicomponent enzyme activation (94). p22^phox phosphorylation does occur on the threonine 147 residue (64), but its precise role in NADPH oxidase activation has not yet been identified. A recent publication indicated that gp91^phox phosphorylation by PKC enhances NADPH oxidase activity as well as increases the binding to Rac2, p47^phox, and p67^phox (90).

Role of cytosolic phox protein phosphorylation during phagocytosis

The best example of subunit phosphorylation and its physiological significance remains the well-understood intracellular signaling pathways involved in p47^phox phosphorylation that induce conformational changes of p47^phox (32, 53) to a state which is accessible for p22^phox binding, allowing for NADPH oxidase activation (1). The phosphorylation of p47^phox provides a control mechanism that modulates NADPH oxidase assembly and/or activity and, thus, prevents inadequate activation. The recent study conducted by Marcoux et al. demonstrated that the phosphorylation of three serine residues in the auto-inhibitory region (AIR) of p47^phox is critical for the release of the phox homology (PX) domain of the protein and its subsequent interaction with p22^phox at the phagosomal membrane (70). Although the PX domain of p47^phox is only a passive requirement for phagosomal superoxide production, experiments using phosphorylation/activation-mimicking p47^phox mutants indicated that adequate serine phosphorylation is required for p47^phox-induced, p40^phox-independent, FcγR-mediated NADPH oxidase activation (65, 117).

The recent discovery of a CGD patient expressing a mutation in the PX domain of p40^phox has firmly established the importance of p40^phox in oxidase activation (72). Controversies over the role of p40^phox phosphorylation exist depending on the experimental approach. In a cell-free system, p40^phox phosphorylated on threonine 154 was shown to inhibit oxidase activity (66). Conversely, the PKCδ-mediated phosphorylation of threonine 154 in p40^phox (but not serine 315) as well as p47^phox translocation to phagosomes is required for full oxidase activation in mouse neutrophils (14). The involvement of NADPH oxidase activity-mediated p67^phox phosphorylation still remains unclear.

It is of interest to investigate whether the phosphorylation of specific phox proteins has a role in modifying the affinity of the subunits for each other or with cytochrome b ₅₅₈ and/or influencing the stability of the complex. Identification of the phosphorylated sites, determination of the phosphorylation status, and site-directed mutagenesis studies should continue to illuminate the significance of phox protein phosphorylation in the regulation of NADPH oxidase during phagocytosis.

Role of phospholipid and p47^phox/p40^phox interactions

Phospholipase D (PLD), which cleaves phospholipids to form phosphatidic acid (PA), is also an important modulator of NADPH oxidase activity. Regier et al. (95) suggested that p22^phox phosphorylation is mediated by PLD-dependent and -independent mechanisms (Fig. 3). PLD-dependent phosphorylation of p22^phox could be activated by a not-yet-identified PA-activated protein kinase, whereas the PLD-independent mechanism is mediated by conventional PKC isoforms (94). The significance of p22^phox phosphorylation may be the promotion of the p22^phox-p47^phox interaction at the membrane (46).

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FIG. 3.

Lipid metabolism. Binding of opsonized particles (OpP) to FcγR or CR3 triggers the activation of several signaling pathways. Stimulation of phosphoinositide 3-kinase (PI3K) results in a downstream inositol phospholipid signaling. It phosphorylates PI(4,5)P₂ to produce phosphatidylinositol 3,4,5 trisphosphate (PI(3,4,5)P₃), which is dephosphorylated by SHIP-1 into phosphatidylinositol 3,4 bisphosphate (PI(3,4)P₂), while inositol polyphosphate 4-phosphatase (4-Pase) hydrolyzed PI(3,4)P₂ to form PI(3)P. Products of inositol phospholipid signaling bind with domain PX of p47^phox and p40^phox, a prerequisite for NADPH oxidase activation. In addition, PI(3,4,5)P₃ activates Rac proteins. In parallel, phospholipase C (PLC), Phospholipase D (PLD), and PLA₂ are also activated. The resulting products, diacylglycerol (DAG) and phosphatidic acid (PA), can activate different types of kinases, participating in the regulation of the NADPH oxidase activity. The level of DAG and PA is dependent on the activity of diacylglycerol kinase (DAGK) and transferase (DAGT). The role of AA underlying NADPH oxidase activation remains unidentified. For details, see text.

3′-phosphoinositides (PI) are also considered pivotal regulators of NADPH oxidase activation by serving as site-specific membrane signals or by modulating cytosolic localization and/or other biological properties of effector proteins. Direct or indirect PI interactions with the subunits p40^phox and p47^phox regulate the NADPH oxidase assembly. Both proteins contain a lipid-binding domain, specifically the PX domain that binds PI with a broad specificity. Studies of PX domain structures by X-ray crystallography have revealed the charged binding sites for stereospecific recognition of their cognate PI (10, 57). The PX domain of p40^phox specifically binds phosphatidylinositol 3-phosphate (PI(3)P), a lipid product of class III phosphoinositide 3-kinase (PI3K), and is required for p40^phox accumulation at the phagosome and assembly/activation of the NADPH oxidase (Fig. 3) (111). Initial evidence regarding the importance of PI(3)P in NADPH oxidase regulation was provided in vitro in semi-recombinant cell-free assays and COS^phox systems (35, 111). Generation of the p40^phox mutant mice expressing the p40^phox which carries a mutation in the PX domain corroborates in vivo that intact binding of PI(3)P to the intact PX domain of p40^phox is required for FcγR-mediated ROS production by professional phagocytes (9). The group of Saito (117) proposed that p40^phox is able to acquire PI(3)P-binding capacity through conformational changes induced by H₂O₂ and functions as a carrier of the cytosolic p47^phox- p40^phox-p67^phox complex. Binding of PI(3)P to p40^phox PX domain has a differential impact on the downstream signaling pathways, depending on the phagocytic receptor subtypes and the type of target particles (34).

The PX domain of p47^phox has two distinct lipid binding pockets: one with preferential affinity for phosphatidylinositol 3,4 bisphosphate (PI(3,4)P₂) and phosphatidylinositol 3,4,5 trisphosphate (PI(3,4,5)P₃), while the other nonspecifically binds anionic phospholipids such as PA and phosphatidylserine (PS) (Fig. 3) (55, 108). The PI-dependent membrane-binding mechanisms of PX domains and their involvement in NADPH oxidase activation have not been clearly identified. However, it has recently been suggested that p47^phox allows p40^phox to acquire PI(3)P binding on targeted membranes that act in cooperation with p47^phox as an adaptor of NADPH oxidase assembly on the phagosome (117).

The role of the p47^phox PX domain in phagosomal NADPH oxidase activation may be selective and may vary greatly depending on the nature of the stimulus. Mutations in the p47^phox PX domain that result in the loss of PI(3,4)P₂ binding does not impair phagosomal membrane recruitment and the normal activity of NADPH oxidase during phagocytosis of a variety of particles in mouse neutrophils. In contrast, plasma membrane NAPDH oxidase activity is reduced in neutrophils expressing p47^phox mutants (65). Thus, although the formation of the phagosomal NADPH oxidase complex is considered similar to the plasma membrane, it seems clear that the regulation of the plasma membrane NADPH oxidase activity differs in terms of signaling pathways from that of phagosomal NADPH oxidase.

Charge-dependent localization of Rac proteins

As for p40^phox and p47^phox, the translocation of positively charged small GTPases to the membrane may involve direct interactions with anionic lipids, mainly PI, PA, and PS. Lipid metabolism regulates the net membrane charge, which is responsible for the selective recruitment of Rac proteins that rely heavily on their own inherent net-positive charge. Based on alterations of lipid metabolism during phagocytosis, a charge-shift mechanism dictates localization patterns to distinct membrane compartments of Rac1 and Rac2 (112). On stimulation, Rac proteins dissociate from RhoGDI, exchange GDP against GTP, and translocate to the membrane where they bind to p67^phox (4). They may either act as an adaptor to ensure correct positioning of p67^phox toward NADPH oxidase or participate in the electron-transfer reaction. The electrostatic forces between Rac1 and the plasma membrane are higher for Rac2 than for the plasma membrane. The higher electrostatic forces result in a preferential association of Rac1 with highly charged actin-rich pseudopods and primarily the localization to the plasma membrane. During phagosome maturation, membrane lipid composition is altered by the partially localized depletion of specific membrane phospholipids (PI(4,5)P₂, PI(3,4,5)P₃) and by the partial decrease in PS at the base of the phagocytic cup and phagosomal membrane (Fig. 4). This generates a membrane environment with a moderate negative charge that correlates with the preferential localization of active Rac2 with the intermediately charged phagosome membrane (69).

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FIG. 4.

Chronological phospholipid-dependent localization of NADPH oxidase subunit. (A) On particle binding, PI(4,5)P₂ transiently accumulates at the site of particle engagement and at the tips of the pseudopods, extending around the phagocytic cup. (B) The concentration of PI(4,5)P₂ decreases rapidly on internalization, and early phagosomes are instead enriched in PI(3,4,5)P₃, generated from PI(4,5)P₂ by PI3K. The disruption of an isoform of inositol hexakisphosphate kinase augments downstream PI(3,4,5)P₃ signaling in phagocytes (88). This increase of PI(3,4,5)P₃ triggers Rac2 activation (dissociation from Rho-GDI). (C) The membrane-associated SHIP-1, which dephosphorylate PI(3,4,5)P₃ yielding PI(3,4)P₂, enhances the early ROS production by transiently increasing PI(3,4)P₂ on the phagosomal membrane (54). Localized alteration of the membrane 3′PI composition affects the recruitment of p47^phox (55, 99) and Rac. (D) Late phagosomes are depleted of PI(4,5)P₂ and predominantly contain PI(3,4,5)P₃.

Contribution of lipid rafts in phagosomal NADPH oxidase activation

Lipid rafts are sphingolipid and cholesterol-enriched insoluble membrane microdomains that are associated with signaling molecules such as receptors. They have the ability to constitute platforms on cell stimulation. These lipid domains have emerged as an important factor in the regulation of NADPH oxidase activity, probably through the control of the efficacy of NADPH oxidase assembly. Indeed, Shao et al. (103) established that NADPH oxidase subunits localize to lipid rafts, which mediate the efficiency of NADPH oxidase coupling to FcγR. A marked negative impact on NADPH oxidase assembly is observed when lipid rafts are disrupted. Later, the work of Jin and co-workers (52) supported this conclusion by highlighting the fact that redox-signaling platforms formed by lipid rafts provide an important driving force for assembling the NADPH oxidase subunits and subsequent NADPH oxidase activation. In addition to the lipid rafts, a member of the Src family tyrosine kinases, which are responsible for the phosphorylation of FcR ITAM motifs and downstream activation of PI3K, has been identified as a regulator of NADPH oxidase recruitment to the phagosomes in rodents (56). This result opens up a new potentiality for our understanding of the mechanisms involved in the regulation of phagosomal NAPDH oxidase activity. In the future, it will be interesting to confirm that Lyn ensures the same function in human neutrophils.

Source of Ca²⁺-mediated phagosomal NADPH oxidase activity

There is no consensus on the source of Ca²⁺, and the question of whether phagosomal oxidase activity requires Ca²⁺ release from intracellular stores or Ca²⁺ influx remains unanswered. Changes in Ca²⁺ are global and not restricted to the phagosomal region but are temporally correlated with NADPH oxidase activity (22). Hallett and co-workers research (22) has given rise to speculation that extracellular Ca²⁺ entry is a rapid event which contributes to [Ca²⁺]_c elevation during C3bi-opsonized zymosan particles-mediated phagocytosis. The fact that knockdown by the specific siRNA of Ca²⁺ release-activated Ca²⁺ modulator 1 (Orai1), an essential pore subunit of Ca²⁺ channels, decreases ROS production on FcγR activation tends to confirm that phagosomal NADPH oxidase activity is dependent on extracellular Ca²⁺ entry (110). However, Ca²⁺ influx per se does not exert a sufficient signal that ensures an optimal NADPH oxidase activation during phagocytosis but instead acts in synergy with other events. Given the importance of phospholipids in phagosomal NADPH oxidase activity, it is reasonable to assume that inositol and Ca²⁺ signaling pathways are interconnected. Observations in B cells that SHIP-1 plasma membrane recruitment on FcγR activation decreases the PI(3,4,5)P₃ level and block Ca²⁺ signals suggest that PI(3,4,5)P₃ is a critical regulator of Ca²⁺ signaling (99). Further, restricting PI(3,4,5)P₃ formation by LY294002, a PI3K inhibitor, has been shown to impair both global Ca²⁺ signal and ROS production triggered by CR3 activation in neutrophils (23). The exact mechanisms linking PI(3,4,5)P₃ formation to Ca²⁺ signals are not yet known. However, this phospholipid may initiate Tec kinase activation, resulting in tyrosine phosphorylation of PLCγ (99) and leading to the formation of inositol 1,4,5-trisphosphate (IP₃), the second messenger responsible for Ca²⁺ release from intracellular stores via the opening of IP₃ receptors during FcγR-mediated phagocytosis (110). Depletion of intracellular Ca²⁺ stores induces activation of the intraluminal Ca²⁺ sensor STIM1 (stromal interaction molecule 1), which, in turn, interacts with Orai1 to allow extracellular Ca²⁺ entry (Fig. 5). This mechanism is known as store-operated Ca²⁺ entry and has been well detailed in research published over the past 20 years (89).

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FIG. 5.

Proposed model for Ca²⁺-dependent phagosomal NADPH oxidase activity. IgG or complement fragments-opsonized particles are recognized by neutrophils through its FcγR or CR3. Phagocytic engagement of these both receptors activate PI3K, leading to the formation of PI(3,4,5)P₃. This phospholipid participates in the activation of PLCγ and PLD-mediated downstream signaling pathways. The generation of IP₃ by PLCγ triggers Ca²⁺ release from intracellular stores (endoplasmic reticulum, ER) via the opening of IP₃ receptors (IP₃-R). PLD is involved in the process of sphingosine kinase-produced sphingosine 1-phosphate (S1P), leading to the depletion of intracellular Ca²⁺ stores. The emptying of intracellular Ca²⁺ stores induces the activation of the Ca²⁺ sensor STIM1, which, in turn, interacts with Orai1 at the plasma membrane where it activates Ca²⁺ channels Orai1 and extracellular Ca²⁺ entry. The resulting [Ca²⁺]_c elevation mediates the recruitment of the cytosolic S100A8/A9 complex to the phagosomal membrane. The translocation of S100A8/A9 allows the transfer of S100A9-binding AA to cytochrome b ₅₅₈, favoring the conformational change of cytochrome b ₅₅₈ and triggering intraphagosomal NADPH oxidase activation.

The role of IP₃ is the subject of much controversy. On the one hand, its role might be dependent on individual FcγR and CR3 for IgG subclasses and complement activity, respectively. On the other hand, as observed in platelets, PI(3,4,5)P₃ may sustain NADPH oxidase activity during neutrophil phagocytosis without an increase in PLC activity but through another lipid-based signaling pathway (85). CR3 could preferentially activate the PLD-dependent cascade, triggering the depletion of Ca²⁺ from intracellular stores via sphingosine-1 phosphate and subsequent STIM1/Orai1-mediated extracellular Ca²⁺ entry (Fig. 5) (79).

In addition to Orai1, Hv1 voltage-gated proton channels could regulate phagosomal Ca²⁺ turnover by preventing the depolarization generated by NADPH oxidase activity, therefore, enhancing the driving force for extracellular Ca²⁺ entry and sustaining NADPH oxidase activity. Initially, proton channels responsible for H⁺ efflux were described as a part of the gp91^phox subunit (46). Later, convincing evidence demonstrated that channels nonrelated to gp91^phox constitute voltage-gated proton channels (21). In this sense, Hv1 channels also preserve physiological membrane electroneutrality by compensating the charge generated by the electrogenic transfer of electrons from NADPH to O₂ ^.− in the cytosol and hydrolysis of NADPH [for review, see (33)] (Fig. 6).

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FIG. 6.

Proposed model for the feed-back mechanism between redox state and phagosomal NADPH oxidase activity. Hv1 voltage-gated proton channels prevent the depolarization due to NADPH oxidase activity and promote [Ca²⁺]_c elevation, probably through Orai1-mediated Ca²⁺ entry (in red). The intraphagosomal Ca²⁺ mobilization sustains NADPH oxidase-produced ROS. H₂O₂ activates TRPM2 channels, which, in turn, trigger membrane depolarization and damp NADPH oxidase activity (in green).

At this point, it is interesting to emphasize that a feedback mechanism could exist between Ca²⁺ homeostasis and NADPH oxidase activity at the phagosomal level. Indeed, it has been shown that extensive membrane depolarization dependent on ROS generation results in a marked decrease of the driving force for Ca²⁺ influx (91, 115). TRPM2, a nonselective and ROS sensitive channel cation found to be expressed in lysosomes (61), might be a putative ROS target linking the redox state of the membrane to Ca²⁺ homeostasis (114, 121). Moreover, TRPM2 possesses the ability to increase [Ca²⁺]_c and the bactericidal activity of neutrophils (49). A model is proposed in Figure 6 that depicts the feedback mechanism for the inactivation of ROS production.

There is a close cooperation between the NADPH oxidase and the Hv1 proton channels to adjust intraphagosomal and cytosolic pH variations. Hv1 proton channels are present in specific granules and accumulated in phagosomes along with the oxidase during FcγR-dependent phagocytosis (80). It has been recently shown that NADPH oxidase-dependent bacterial killing was significantly reduced in Hv1-deficient mice neutrophils in vitro (87, 93). During phagocytosis-mediated NADPH activation, the consumption of NADPH produces H⁺ that acidifies the cytosol (74). The acidification and the electrogenic translocation of electrons into the phagosome are perfectly compensated by Hv1 proton channels, as they are activated by intracellular acidification and depolarizing voltages (75). Surprisingly, the intraphagosomal pH remains near neutral for several minutes, possibly due to several mechanisms such as the net consumption of luminal H⁺ during the dismutation of O₂ ^.− to hydrogen superoxide, a large passive proton leak to the cytosol, preventing accumulation in the lumen and a reduced insertion of V-ATPases in the phagosome membrane, resulting in lower rates of H⁺ efflux (Fig. 7) (33, 51).

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FIG. 7.

Correlation between NADPH oxidase and Hv1 activities. Phagocytosis-triggered NADPH oxidase activation leads to a flux of electrons that depolarizes the membrane and an accumulation of protons, both events activating the Hv1 proton channels. Hv1 promotes proton influx into the phagosomal lumen, counteracting cytosol acidification and favoring sustained NADPH oxidase activity. Intraphagosomal accumulation of protons during early phagosome maturation is prevented by three mechanisms: (i) Consumption of protons for the production of ROS species, (ii) ROS-dependent passive leak of protons to the cytosol (51), and (iii) reduced fusion of V-ATPases-containing granules to the phagosome membrane, resulting in low rates of H⁺ flux.

S100A8/A9: the link between Ca²⁺ and NADPH oxidase

Over the last decade, the phagocyte-specific Ca²⁺-binding S100A8 and S100A9 proteins have been proposed as essential regulators of the plasma membrane NADPH oxidase activity. These proteins are abundantly expressed in the cytosol of neutrophils and are able to form Ca²⁺-dependent heterocomplexes, with heterotetramers being a probable prerequisite for their biological activities in myeloid cells (62). S100A8 and S100A9 have been proposed as essential regulators that exert their role through interactions with NADPH oxidase subunits (8).

The addition of Ca²⁺-loaded S100A8/A9 to the reconstituted assembled NADPH oxidase complex prepared with neutrophil cytochrome b ₅₅₈ and B lymphocyte cytosol is able to increase the constitutive activity of cytochrome b ₅₅₈ in the absence of any stimulus. Initially, S100A8/A9 has been designated as a positive allosteric effector of NADPH oxidase activity that interacts preferentially with p67^phox and increases its affinity for cytochrome b ₅₅₈. However, the increased affinity of p67^phox for cytochrome b ₅₅₈ as inferred by S100A8/S100A9 was confirmed by Berthier and co-workers (8) in a semi-recombinant cell-free system, whereas S100A8/S100A9 appear to also interact directly with cytochrome b ₅₅₈. Observations conducted on structural conformation changes by atomic force microscopy underlie the fact that the S100A8/A9 complex is able to enhance or induce a transition from an inactive to an active conformation state of cytochrome b ₅₅₈. Preincubation of S100A8/S100A9 in the absence of Ca²⁺ led to an interaction with cytochrome b ₅₅₈ but not to a conformational change, allowing ROS production. Thus, the relevant role for S100A8/A9 in NADPH oxidase activity is dependent on Ca²⁺ (7) and is probably mediated via S100A8/A9 translocation to the membrane where NADPH oxidase is activated (97, 100, 101). An elevation of [Ca²⁺]_c is necessary for S100A8/A9 redistribution to the plasma membrane; thus, intracellular Ca²⁺ store depletion appears to be substantially responsible for this phenomenon (100).

From these observations, it is logical to question whether S100A8/A9 could also constitute the link between [Ca²⁺]_c elevation and phagosomal NADPH oxidase activity. Different research groups have provided indirect evidence for such a speculation. Kumar et al. (59) showed that phagocytosis was associated with a rapid reduction of cytoplasmic immunostaining of the S100A8 homodimeric form, and a Ca²⁺ dependence of S100A8/A9 translocation in cytoskeletal structures was reported (97). Direct evidence that S100A8/A9 constitute the molecular switch between [Ca²⁺]_c elevation and phagosomal NADPH oxidase activity has been provided by a recent work of Steinckwich et al. (110). These authors established that S100A8/A9 siRNA knockdown decreases intraphagosomal ROS production. Further, on FcγR-induced [Ca²⁺]_c elevation, endogenous S100A8/A9 is recruited to the phagosomal membrane at the beginning of the formation of the phagocytic cup and persists throughout the zymosan internalization process. Therefore, extracellular Ca²⁺ entry-mediated S100A8/A9 phagosomal membrane recruitment serves as a determinant for the NADPH oxidase activity. Via a mechanism similar to that proposed at the plasma membrane level (8, 81), S100A8/S100A9 could deliver AA to the phagosomal membrane (Fig. 5), increasing its local concentration and favoring the conformational change of cytochrome b ₅₅₈. This hypothesis requires confirmation, but it may provide a foundation for further investigation of the link between S100A8/A9 and phagosomal NADPH oxidase. In the near future, it will also be of interest to (i) identify phagosomal NADPH oxidase subunits involved in direct interactions with S100A8/A9 and (ii) characterize the additional signaling pathways leading to S100A8/A9 phagosomal membrane recruitment with an emphasis on phospholipid and phosphorylation signaling cascades.

The pro-inflammatory role of S100A8/A9 is not restricted to the intracellular level. Neutrophils are known to secrete S100A8/A9 that are involved in autocrine/paracrine regulatory mechanisms underlining the inflammatory process (30, 92). In a variety of cell types, this dual action is mediated through the engagement of Receptor for Advanced Glycation End (RAGE) products signaling (47). This signaling contributes to the activation of p38 MAPK and the downstream effector NF-κB (29). S100A8 and S100A9 have recently been identified as target genes of NF-κB (76), which regulate their expression, thus allowing them to fulfill their intracellular roles (Fig. 8). In parallel, exogenous S100A9 may have the ability to induce NADPH oxidase activation, although it mainly stimulates neutrophil microbicidal activity by promoting phagocytosis (106). Further, other RAGE ligands have been associated with a modulation of bacterial destruction by neutrophils and activation of NADPH oxidase. Definitely, the prototypical RAGE ligand AGE has been found to increase bacterial killing by neutrophils and activation of NADPH oxidase, likely through the phosphorylation of the p40^phox subunit. In contrast, high mobility group box 1 diminished the ability of neutrophils to destroy pathogens and NADPH oxidase activation (113).

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FIG. 8.

Possible feed-forward signaling mediated by S100A8/A9 during phagocytosis. Receptor for Advanced Glycation End (RAGE) ligation by S100A8/A9, on the neutrophil surface, leads to the activation of the transcription factor NF-(B, which, in turn, induces the production of S100A8 and S100A9. The S100A8/A9 complex is recruited to the phagosomal membrane to regulate NADPH oxidase activation or is secreted in the extracellular environment. Extracellular S100A8/A9 amplifies the pro-inflammatory response by either activation of neutrophils (autocrine mode of action) or other inflammatory cell types (paracrine mode of action).

In light of these results, an intriguing possibility arises that S100A8/A9 secreted by neutrophils on opsonized zymosan stimulation bind to RAGE and regulate neutrophil phagosomal NADPH oxidase activity (Fig. 8). Kumar et al. (59) have hypothesized that the rapid loss of cytoplasmic immunoreactivity for homodimeric S100A8 was the consequence of S100A8 release by active secretion on opsonized zymosan stimulation. Using the same line of evidence, Guignard et al. (43) showed that S100A8 and S100A9 were recruited to the plasma, underlining the eventual and subsequent S100A8/A9 secretion after opsonized zymosan. However, these authors have provided no clear indication for a secretion of S100A8 and S100A9 proteins. While the release of S100A8 and S100A9 upon soluble stimuli is well established, to our knowledge, no direct evidence for the release of neutrophil S100A8 and S100A9 in response to particulate stimuli has yet been convincingly provided. Further investigations are, thus, compulsory. Understanding the S100A8/A9-RAGE-NADPH oxidase axis, in an autocrine/paracrine fashion, potentially offers some interesting perspectives for providing a more comprehensive mechanism for the regulation of the phagosomal NADPH oxidase.

Monitoring of phagosomal ROS production and Ca²⁺ mobilization

To understand the relationship between intracellular Ca²⁺ mobilization and NADPH oxidase activity, quantitative concomitant measurement of phagosomal NADPH oxidase activity and [Ca²⁺]_c changes with sufficient resolution is not only necessary but also a challenge. One of the issues is the difficulty of distinguishing the contribution of [Ca²⁺]_c elevation for the phagosomal oxidative event from earlier phagocytic events (chemotaxis, phagocytic cup formation, engulfment, and phagosome closure). Commonly used techniques for following intraphagosomal ROS production are provided in Table 3 [for a more detailed description, see (38, 96, 116, 123)]. An original technique was developed by Dewitt and co-workers (22) in which C3bi-opsonized particles labeled with an oxidant-sensitive probe (2′,7′-dichlorodihydrofluorescein [DCFH₂]) were presented to neutrophils at a defined time and location using micromanipulation. Later, Steinckwich et al. (110), using fluorescence light microscopy, which allows for the detection of intraphagosomal ROS production, measured simultaneously [Ca²⁺]_c elucidating the relationship between phagosomal NADPH oxidase activity and changes in [Ca²⁺]_c. Classical indicators used to monitor [Ca²⁺]_c changes remain fluorescent dyes such as fluo-4 or fura-2, but genetically encoded probes are being developed to follow Ca²⁺ amplitude and kinetics at specific subcellular locations and improve Ca²⁺ imaging resolution [for review, see (20)].

To overcome the lack of sensitivity, and problems with auto-oxidation and quenching observed with the fluorescein derivative DCFH₂, research has been devoted to the development of a specific probe to improve the detection and measurement of intracellular H₂O₂ production. Genetically encoded probes, which can be targeted at specific subcellular locations (96), represent potentially useful tools for monitoring H₂O₂ production within the phagosomal compartment.