Correction to: Dead Cas9–sgRNA Complex Shelters Vulnerable DNA Restriction Enzyme Sites from Cleavage for Cloning Applications,by Saifaldeen M et al. The CRISPR Journal,2021;4(2):275–289;DOI: 10.1089/crispr.2020.0134
Available accessCorrectionFirst published online December, 2021
Correction to: Dead Cas9–sgRNA Complex Shelters Vulnerable DNA Restriction Enzyme Sites from Cleavage for Cloning Applications,by Saifaldeen M et al. The CRISPR Journal,2021;4(2):275–289;DOI: 10.1089/crispr.2020.0134
In the April, 2021 issue of The CRISPR Journal (vol.4, no.2; 275–289) the article entitled “Dead Cas9–sgRNA Complex Shelters Vulnerable DNA Restriction Enzyme Sites from Cleavage for Cloning Applications,” by Saifaldeen et al. requires correction.
The panels listed in Fig. 5B of the figure were incorrect. An error in section E of the caption has been updated also and is displayed in bold. These errors had no effect on the scientific content or conclusions. The changes made do not affect the conclusions drawn either in this experiment or in this study.
The following pages explain the original and corrected figure.
dCas9–sgRNA-BbsI-F1R1 multiplexes efficiently prevent restriction cleavage of two internal BbsI cut sites in the insert I-3 to promote full-length cloning into the vector V-3. (A) Schematic illustration showing the strategy used for blocking two internal BbsI sites in the full-length insert I-3 to allow its cloning into the V-3 vector. (B) Insert I-3 PCR amplicon (200 ng) subjected to single or dual RE digestion (KpnI and BbsI) without or with preincubation with increasing concentrations of either dCas9–sgRNA-BbsI-F1R1 or -F1R2. (C) Recognition, cleavage, sgRNAs, and protospacer adjacent motifs targing BbsI sites I and 2. (D) Releasing of the dRNPs from the DNA using the indicated treatments. (E) Analysis of KnpI and BbsI digested and purified vector V-3 and the digested and purified insert I-3. (F) SacI unique digestion of one potential pV-3-I-3 clone carrying the full-length insert I-3. Samples were run on 1.5% agarose gel stained with SYBR Safe Dye. Panels 5A and 5C: created using Biorender.com
The figure has been revised to:
The legend has been revised to:
dCas9–sgRNA-BbsI-F1R1 multiplexes efficiently prevent restriction cleavage of two internal BbsI cut sites in the insert I-3 to promote full-length cloning into the vector V-3. (A) Schematic illustration showing the strategy used for blocking two internal BbsI sites in the full-length insert I-3 to allow its cloning into the V-3 vector. (B) Insert I-3 PCR amplicon (200 ng) subjected to single or dual RE digestion (KpnI and BbsI) without or with preincubation with increasing concentrations of either dCas9–sgRNA-BbsI-F1R1 or -F1R2. (C) Recognition, cleavage, sgRNAs, and protospacer adjacent motifs targing BbsI sites I and 2. (D) Releasing of the dRNPs from the DNA using the indicated treatments. (E) Analysis of KpnI and BbsI digested and purified vector V-3 and the digested and purified insert I-3. (F) SacI unique digestion of one potential pV-3-I-3 clone carrying the full-length insert I-3. Samples were run on 1.5% agarose gel stained with SYBR Safe Dye. Panels 5A and 5C: created using Biorender.com
The authors apologize for this error and for any inconvenience caused.
The online version of the article has been corrected.
