A Combined DNA Vaccine Encoding BCSP31,SOD,and L7/L12 Confers High Protection Against Brucella abortus 2308 by Inducing Specific CTL Responses

We constructed a combined DNA vaccine comprising genes encoding the antigens BCSP31, superoxide dismutase (SOD), and L7/L12 and evaluated its immunogenicity and protective efficacy. Immunization of mice with the combined DNA vaccine offered high protection against Brucella abortus (B. abortus) infection. The vaccine induced a vigorous specific immunoglobulin G (IgG) response, with higher IgG2a than IgG1 titers. Cytokine profiling performed at the same time showed a biased Th1-type immune response with significantly increased interferon-γ and tumor necrosis factor-α stimulation. CD8⁺, but not CD4⁺, T cells accumulated at significantly higher levels after administration of the vaccine. Granzyme B–producing CD8⁺ T cells were significantly higher in number in samples prepared from combined DNA–vaccinated mice compared with S19-vaccinated mice, demonstrating that the cytotoxicity lysis pathway is involved in the response to Brucella infection. The success of our combined DNA vaccine in a mouse model suggests its potential efficacy against brucellosis infection in large animals.