Establishment of Minimum Operational Parameters for a High-Volume Static Chamber Steam Pasteurization System (SPS 400-SC ™ ) for Beef Carcasses to Support HACCP Programs

A static chamber steam pasteurization unit (SPS 400-SC^™) was installed in a high-volume commercial beef slaughter facility. The SPS 400-SC consists of a three-phase carcass treatment cycle of water removal, steam pasteurization, and water chilling. Seven chamber temperatures (71.1, 73.9, 76.7, 79.4, 82.2, 85.0, and 87.8°C) were evaluated at the midline area of pre-rigor beef carcasses. For each temperature evaluated, 20 carcass sides were randomly selected and aseptically sampled by tissue excision immediately before and after steam pasteurization to determine total aerobic bacteria, Enterobacteriaceae, generic E. coli, and total coliform populations. The 87.8 and 85.0°C treatment temperatures were highly effective at reducing total aerobic bacterial populations, with log₁₀ reductions of 1.4 and 1.5 CFU/cm², respectively, from pretreatment mean population levels of 1.7 and 1.9 log10 CFU/cm². These temperatures also reduced Enterobacteriaceae, total coliforms, and generic E. coli to undetectable levels (<0.4 CFU/cm²) on all carcasses sampled. Treatment at 82.2 was marginally effective at reducing bacterial populations, while 71.1, 73.9, 76.7, and 79.4°C treatments were ineffective at reducing microbial populations. In a Hazard Analysis Critical Control Points (HACCP)–based system employing steam pasteurization of carcasses as a critical control point, a critical limit of 85.0°C as a minimum chamber temperature should be established, with a targeted operating temperature of 87.8°C providing optimum antimicrobial activity.