Abstract
A complete cDNA clone of murine interferon-γ (MuIFN-γ) was obtained by recombining two appropriate segments from partial cDNA clones originally identified by colony hybridization with rat IFN-γ chromosomal gene fragments as probes. An expression vector was constructed in which the cDNA was placed under control of the SV40 early promoter. Transient expression of MuIFN-γ was obtained by transformation of COS-1 cells. Subsequently, this interferon expression unit was linked to a vector containing a dihydrofolate reductase (DHFR) modular gene and used to transform DHFR--CHO cells. Cell clones were selected that constitutively produce an interferon activity which by several criteria was found to be indistinguishable from natural, splenocyte-derived MuIFN-γ.
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