Subcutaneous injection of antigen for immunization. Day 0, mice received initial inoculation of 20 μg, followed by four boosts given on days 28, 56, 84, and 112.
Parental Cell Line Used for Fusion
P3X-Ag 8.653.
Selection and Cloning Procedure
ELISA using gamma-irradiated Acinetobacter baumannii 19606. Hybridoma clones were sub-cloned by limiting dilution.
Heavy and Light Chains of Immunoglobulin
50 kDa heavy chain and 25 kDa light chain, identified by Coomassie-stained SDS-PAGE gel.
Specificity
ELISA-tested monoclonal antibody against irrelevant proteins and different Acinetobacter baumannii strains.
Specific Antigen Identified
N/A.
Availability
Tissue culture supernatant
Yes
No ✓
Ascitic fluid
Yes
No ✓
Hybridoma cells
Yes ✓
No
References
1.
CorbettC, BaigA, and CabralT: Development and characterization of monoclonal antibodies for the rapid detection of Acinetobacter baumannii. Monoclon Antibod Immunodiagn Immunother, 2014; 33:291–298.
