The antibody was not immunized. Cloning was performed from the human naïve antibody display phage library (1.1×1010cfu).1
Parental Cell Line Used for Fusion
There was no fusion performed. Recombinant human antibody was expressed in HEK293E cells.
Selection and Cloning Procedure
scFv anti-uPA antibody A8 was cloned into pcDNA-hFc to convert scFv-minibody.
Heavy and Light Chains of Immunoglobulin
VH: 402 bp, VH3-30 family.
VL: 324 bp, Vλ2 family.
Specificity
Antigen binding: flow cytometry.
Agonistic activity: uPA enzyme assay using the substrate pyro-Glu-Gly-Arg-pNA.
Specific Antigen Identified
Human urokinase-type plasminogen activator (uPA).
Availability
Tissue culture supernatant
Yes ✓
No
Ascitic fluid
Yes
No ✓
Hybridoma cells
Yes
No ✓
References
1.
BaeKB, JeongYJ, WonHJ, HongKH, ChoiIW, SeoSK, and ParkSG: A human monoclonal antibody scFv to urokinase plasminogen activator. Hybridoma, 2010; 29:147–152.
2.
SongH, LeeA-Y, JungH, ChoiJH, RohK, HaS, KimKD, BaeKB, KangMS, ParkSJ, ChoiIW, SeoSK, and ParkSG: A8, an anti-uPA agonistic antibody, promotes metastasis of cancer cells via ERK pathway. Monoclon Antib Immunodiagn Immunother, 2014; 33(5):312–318.
