Wistar Kyoto rats (Charles River Laboratories, Yokohama, Japan) were injected via the hind footpads with 100 μL of an emulsion containing 1×107 of FRβ-expressing RBL2H3 and Freund's complete adjuvant.
Parental Cell Line Used for Fusion
NS-1.
Selection and Cloning Procedure
HAT selection and limiting dilution.
Heavy and Light Chains of Immunoglobulin
Rat immunoglobulin IgG2a.
Specificity
Reactivity with both human folate receptor α-expressing KB and folate receptor β-expressing B300-19, but not with folate receptors α and β negative B300-19.
Specific Antigen Identified
Human folate receptors α and β.
Availability
Tissue culture supernatant
Yes ✓
No
Ascitic fluid
Yes
No ✓
Hybridoma cells
Yes
No ✓
References
1.
NagaiT, FurushoY, LiH, HasuiK, MatsukidaS, SueyoshiK, YanagiM, HataeM, TakaoS, and MatsuyamaT: Production of a high affinity monoclonal antibody reactive with folate receptors alpha and beta. Monoclon Antib Immunodiagn Immunother, 2015; 34(3);213–221.
