Monoclonal Antibody 4E3-2F7 Antimouse Dermokine-β/α

Antigen Used for Immunization

Mouse dermokine-α amino terminus peptide.

Method of Immunization

Injection into the rear footpads of a rat with Freund's complete adjuvant.

Parental Cell Line Used for Fusion

Mouse myeloma SP2/0-Ag14 cells.

Selection and Cloning Procedure

Hybridoma cells were plated onto 96-well plates and cultured in hypoxanthine-aminopterin-thymidine selection medium.

Heavy and Light Chains of Immunoglobulin

Rat IgG2b, k.

Specificity

Western blot, immunofluorescence, ELISA.

Specific Antigen Identified

Mouse dermokine-β/α.

Availability