Monoclonal Antibody Specific for Ovarian Tumor Domain-Containing Protein 1

Abstract

Antigen Used for Immunization

mOtud1 (full length)

Method of Immunization

Rat lymph node method

Parental Cell Line Used for Fusion

SP2/0-Ag14

Selection and Cloning Procedure

HAT selection medium

Heavy and Light Chains of Immunoglobulin

7G4 was determined as rat IgG2aκ

Specificity

7G4 detected both human and mouse OTUD1 (overexpression)

Specific Antigen Identified

hOTUD1 (1–290)

Availability

	Yes	No
Tissue culture supernatant	[✓]	[ ]
Ascitic fluid	[ ]	[✓]
Hybridoma cells	[ ]	[✓]

References

Oikawa

, Shiota

, Goto

, Komakura

, Wanibuchi

, and Tokunaga

. Generation of rat monoclonal antibodies against a deubiquitinase, Ovarian Tumor Domain-Containing Protein 1. Monoclon Antib Immunodiagn Immunother, 2018; 37:180–184.