Identification of Four Genes of the Brucella melitensis ATP Synthase Operon F 0 Sector: Relationship with the Rhodospirillaceae Family

ABSTRACT

We have determined the nucleotide sequence of a cloned DNA fragment from the human and animal pathogen Brucella melitensis. Four genes were identified from a 4069 bp fragment, corresponding to the B. melitensis a, c, b′, and b subunits of the ATP synthase F₀ sector operon. A duplicated and divergent copy of the b-subunit gene was observed. This feature has been found only in photosynthetic bacteria and chloroplasts. In addition, the gene cluster was separated from the F₁ sector, a characteristic described only for the Rhodospirillaceae family.