New Self-Assembling Multifunctional Templates for the Biofabrication and Controlled Self-Release of Cultured Tissue

Synthesis of PAs

PAs were custom synthesized by CS Bio as >95% pure trifluoroacetic acid salts and their molecular weight confirmed by electrospray mass spectrometry. Lyophilized C₁₆-TPGPQG↓IAGQRGDS (_mfPA;↓indicates cleavage site for MMP1) and C₁₆-ETTES (_dPA) ³² were weighed and dissolved as single or binary component solutions in ultrapure water from a Barnstead Nanopure system to the desired concentrations. For binary PA systems, _mfPA and _dPA were mixed in mol/mol ratios. The C₁₆-GGGRGDS (_sfPA), ²⁴ C₁₆-TPGPQG, and IAGQRGDS molecules were similarly prepared as single component solutions.

Cell culture

Corneal tissues were obtained as by-products of grafting procedures and kindly provided by Mr. Martin Leyland, BSc, MD, FRCOphth, following informed consent. All experiments were approved by the Royal Berkshire NHS Foundation Trust (RBFT) R&D Office and in accordance with RBFT and MHRA ethics guidelines. Human corneal stromal fibroblasts (hCSFs) were isolated from epithelia-depleted corneal rings and cultured as previously described. ³³ For additional details, see the Supplementary Materials and Methods section (Supplementary Data are available online at www.liebertpub.com/tea).

Preparation of PA coatings

To produce thin, dry film coatings, 100 μL aliquots from PA solutions at 1×10⁻⁵–10⁻² M were spotted as≈1.5 cm² drops onto standard or low-attachment, tissue culture plastic surfaces (Nunc) and left to dry overnight at room temperature inside an aseptic, Class II cell culture cabinet. Resulting films were washed thrice with sterile phosphate-buffered saline (PBS) just before cell seeding. Stability of PA coatings was assessed by imaging film coatings upon drying (day 0) and after incubation in serum-free medium (SFM) at 37°C for 7 days using a LumaScope inverted light microscope (Etaluma). Conditioned medium enriched with endogenous MMPs (cSFM) was prepared by incubating confluent hCSFs with SFM for 5 days immediately before use in stability assays. MMP-free conditioned medium (_RASFM) was similarly produced while adding 1×10⁻⁵ M of all-trans retinoic acid (RA; Sigma-Aldrich) every other day to inhibit hCSFs from expressing MMPs. ³³

Adhesion and proliferation assays

To evaluate the biocompatibility and bioactivity of _mfPA, single and binary PA coatings were produced on low-attachment, tissue culture plastic surfaces and then seeded with 1.5×10⁴ hCSFs·cm² in serum-free culture medium supplemented with 1×10⁻⁵ M RA. Cell adhesion and proliferation were evaluated using the AlamarBlue assay (Life Technologies) according to the manufacturer's instructions (see the Supplementary Materials and Methods section). Water-treated polystyrene surfaces were used as uncoated control. To assess adhesion specificity, hCSFs were incubated for 30 min with 5×10⁻⁵ M of _dPA (mock), _sfPA, anti-αVβ5 integrin antibody (ab24694; Abcam), or cyclo[Arg-Gly-Asp(D-Phe)-NMe-Val] (cRGD; CS Bio) before seeding, and the cell number was evaluated 24 h postseeding. All experiments were performed in triplicate.

Analysis of gene marker expression

Cells seeded at 1.5×10⁴ hCSFs·cm² on 1.25 mM _mfPA:_dPA at a 15:85 mol/mol ratio or _dPA templates coating normal cell culture plates were grown for 21 days in serum-free culture medium supplemented with 1×10⁻⁵ M RA, and then harvested for mRNA isolation by standard Trizol extraction (Life Technologies) and evaluation of gene expression as previously described^27,33 (see the Supplementary Materials and Methods section).

Biofabrication and self-release of 3D tissue

Coatings produced on low-attachment cell culture plates using 1.25 mM _mfPA:_dPA at 15:85 mol/mol served as templates for adhesion, proliferation, and stratification of hCSFs. Cells were seeded (5×10⁴ cells·cm²) and cultured for 90 days in SFM+1×10⁻⁵ M RA to allow the fabrication of an ECM-rich tissue, and then maintained in nonsupplemented SFM for 3 days to elicit MMP expression and consequent tissue self-release. Tissue detachment was monitored by following removal of RA from the culture medium by time-lapse bright-field microscopy using a remote-operated LumaScope inverted microscope inside the tissue incubator.

Reattachment assays

Self-released tissues (R1) were sampled using a reusable 3-mm Harris micropunch (Sigma-Aldrich) and then reconditioned in RA-supplemented SFM for 3 days to suppress MMP expression endogenous to the tissue. Reconditioned tissues were then transferred to new uncoated or _mfPA:_dPA-coated low-attachment plates and maintained in RA-supplemented SFM. A glass coverslip was used to hold the tissue plane in close contact with the surface. After 7 days, glass coverslips were removed and tissues were washed with PBS and imaged from above and diagonally using a Pentax Optio 750Z digital camera (Pentax). Tissues attached to the _mfPA:_dPA coating were then released as before by removal of RA from the culture medium. These second-time self-released tissues (R2) were again sampled, and the reattachment/redetachment process repeated for a third time (R3). Samples were used immediately after punching to evaluate cell viability using the Live/Dead Cell double staining kit (CalBioChem; Merck) according to the manufacturer's instructions. Calcein- and propidium iodide-stained cells were imaged using an Axio Imager fluorescence microscope (Zeiss) at λ_em=515 and 620 nm, respectively. Quantification of total viable cells was performed by analyzing 10 different fields per sample from 3 independent samples.

Contraction assays

Free-floating self-released tissues, R1, R2, and R3, were imaged (day 0) using a Nikon D90 digital camera (Nikon) and then incubated in cell culture medium containing 5% fetal bovine serum (FBS) to elicit myofibroblast differentiation of hCSFs. After 7 days, tissues were again imaged (day 7) and contraction evaluated by calculating the ratio between the initial and final areas for each tissue using the ImageJ v1.46 software.

Statistical analysis

Error bars represent the standard deviation of the mean, analyzed a priori for homogeneity of variance. Replicates from each independent experiment were confirmed to follow a Gaussian distribution, and differences between groups were determined using one- or two-way analysis of variance, followed by Bonferroni's multiple comparison post hoc test. Significance between groups was established for p<0.05, 0.01, and 0.001, with a 95% confidence interval, and R ² values of 0.7585, 07591, 0.9031, 0.9291, and 0.9814 for the proliferation, gene expression, contraction, viability, and MMP1 expression assays, respectively.

Biophysical characterization of the PAs

The _mfPA was solubilized from lyophilized powder in ultrapure water and its critical aggregation concentration (c.a.c.) determined by Thioflavin T (ThT) fluorescence was 1.1×10⁻³ M (Fig. 1b, upper panel). Fourier transform infrared (FTIR) spectroscopy experiments above the c.a.c. showed that _mfPA self-assembled with a secondary β-sheet structure, as is denoted by the positive band at 1620 cm⁻¹ (Fig. 1c), suggesting its arrangement into highly organized structures. ²⁴ This was supported by transmission electron microscopy (TEM), which showed that _mfPA forms well-defined polydisperse nanotapes, 528±83 Å wide (Fig. 2a), displaying 5.6±0.8 Å-thick stripes along the main axis of the fiber (Fig. 2a, left inset), expectedly associated with the internal lamellar structure of the nanotapes. ²⁴

OPEN IN VIEWER

FIG. 2.

Specific degradation of _mfPA by MMP1. (a) Transmission electron micrographs of self-assembled structures comprising _mfPA and _mfPA:_dPA. In aqueous solution, these PA systems formed well-defined polydisperse nanotapes (control) that degrade when treated with a protease able to recognize the enzyme-responsive sequence of _mfPA (+MMP1). Scale bars, 500 nm (insets, 50 nm). (b) Enzyme-catalyzed cleavage of _mfPA in solution. The products of the reaction with enzymes that cleave (+MMP1) or are unable to recognize the enzyme-responsive sequence of _mfPA (+MMP7) were analyzed by mass spectrometry and compared with enzyme-free control (+buffer).

The sensitivity of _mfPA (m/z 1578.87) to specific cleavage by metalloproteases was analyzed by mass spectrometry after incubation of _mfPA nanotapes with MMP1. This enzyme specifically recognized the cleavage sequence designed for _mfPA, producing the expected peptide fragment, IAGQRGDS, detected at m/z 803.43 (Fig. 2b). A smaller moiety corresponding to the GQRGDS peptide was also detected at m/z 619.3, indicating some residual proteolytic cleavage or degradation. However, the cleavage product, C₁₆-TPGPQG, was not detected by mass spectrometry. This product self-assembled above 8.6×10⁻⁵ M (Supplementary Fig. S1) and formed micelle-like structures (Supplementary Fig. S2) similar to those observed in MMP1-treated _mfPA (Fig. 2b). In contrast, no self-assembly was detected for the IAGQRGDS peptide (Supplementary Fig. S1). These results suggested that, upon cleavage of the _mfPA by MMP1, the RGDS-containing peptide is released into the medium, whereas the newly formed C₁₆-TPGPQG fragment remained assembled within the pre-existing nanotapes, forming distinct self-assembling structures. The specificity of cleavage was further tested by incubating _mfPA with proteolysis buffer only or with recombinant MMP7. MMP7 was previously shown to be expressed by various cell types, including hCSFs, ³⁴ but unable to target the MMP-cleavage sequence included in the _mfPA.^29,35 No cleavage products were obtained after these treatments (Fig. 2b), demonstrating that the cleavable motif of the _mfPA is recognized only by specific proteases.

Several studies have shown that the bioactivity of PAs containing cell adhesion motifs can be augmented using diluent PA molecules to space the bioactive molecules in the assembled nanostructure,^27,36 thus creating binary PA systems that minimize interaction between bioactive motifs. ³² Recently, we have used the nonbioactive, negatively charged C₁₆-Glu-Thr-Thr-Glu-Ser molecule (hereby designated _dPA) as such a diluent to achieve the optimal cell adhesion activity of an RGDS-containing PA. ²⁷ Similarly, we produced a binary PA system by combining _mfPA with _dPA in water. ThT fluorescence analysis showed that the c.a.c. of the mixed system was five times lower than single-system _mfPA (Fig. 1b), indicating that the aggregation process was driven by the _dPA, probably due to its higher degree of hydrophobicity. FTIR for _mfPA:_dPA denoted a β-sheet secondary structure with a positive band at 1620 cm⁻¹ (Fig. 1c). The existence of a single c.a.c. for the binary _mfPA:_dPA system suggested an interaction between _mfPA and _dPA molecules. Additional evidence was found by TEM, where the self-assembly of the mixed binary system led to the formation of nanotapes, 254±41 Å wide (Fig. 2a), displaying stripes similar to those observed for single-system _mfPA (Fig. 2a, right inset). Furthermore, these nanotapes were also sensitive to cleavage by the proteolytic activity of MMP1 (Fig. 2a, bottom right panel). Altogether, these results indicate that _mfPA:_dPA coassembled and formed homogeneous nanostructures where the bioactive enzyme-responsive _mfPA is interspersed with _dPA molecules.

Bioactivity of the PA templates

The bioactivity of single and mixed PA systems was tested by evaluating the potential of _mfPA and _mfPA:_dPA to promote integrin-dependent cell attachment and direct cell phenotype. For that, various concentrations of PAs in solution were drop-spotted and dry-coated onto low-attachment cell culture plates, allowing the formation of thin film coatings (Fig. 3). The stability of these films was tested after incubation with SFM previously conditioned by hCSFs (cSFM, containing secreted MMPs). As controls, MMP-free media, that is, fresh SFM or medium conditioned in the presence of all-trans RA (_RASFM), were used. RA is a metabolite of vitamin A that suppresses MMP expression while enhancing ECM production from various cell types, namely hCSFs ³³ and skin fibroblasts. ³⁷ The results demonstrated that, after 7 days in cSFM, _mfPA and _mfPA:_dPA films were partially removed with considerable fraying and degradation of the edges (Fig. 3, cSFM). In contrast, when incubated with MMP-free media (Fig. 3, SFM or _RASFM), the films remained intact and comparable as before incubation (Fig. 3, day 0). This effect was shown to be specific to films containing the _mfPA molecule as films comprising a single function PA, _sfPA (i.e., with the RGDS motif, but without the MMP-cleavable sequence ²⁷ ), were not degraded in cSFM (Fig. 3). These results further suggested that the supplementation of cell culture medium with RA constituted a simple and reliable method to control the cleavage of _mfPA, and consequently, to regulate the ability of _mfPA-containing coatings to provide a template for cell attachment. Moreover, the use of RA as a medium supplement was physiologically relevant as this metabolite has been shown to play a fundamental role in the correct development, function, and regeneration of many organs, ³⁸ as well as in cancer prevention. ³⁹ As such, RA was supplemented to SFM in subsequent cell culture experiments, unless otherwise noted.

OPEN IN VIEWER

FIG. 3.

Stability of PA coatings. Self-assembled _mfPA and _mfPA:_dPA structures dry-coated on cell culture surfaces produced continuous films (day 0) that degraded after incubation with conditioned culture medium (cSFM), but remained stable in MMP-free media (serum-free medium [SFM] or _RASFM). This effect was specific to _mfPA-containing coatings and not to functional PAs without an MMP-responsive sequence (_sfPA). Scale bars, 20 μm.

The optimal bioactivity of the film coatings was assessed for _mfPA mixed with _dPA in ratios from 0:100 (_dPA only) to 100:0 mol/mol (_mfPA only) and at final concentrations of 1×10⁻⁵–10⁻² M. The activity of the different PA coatings was assessed by counting the number of attached hCSFs after 7 days in culture. Low-attachment plates were used as the underlying culture surface to minimize cell adhesion on uncoated areas. The results showed that all coatings containing _mfPA were able to support cell adhesion and proliferation, whereas _dPA coatings (0:100 ratio) and uncoated controls were not (Fig. 4). Cells grown on biofunctional adhesive coatings assumed an extended fibroblast-type morphology similar to that presented by cells attached to standard polystyrene culture surfaces, but those on _dPA remained round shaped (Fig. 4a). The adhesive activity of the coatings was shown to be dependent on _mfPA concentration up to 1.25×10⁻³ M and on the PA ratio, with an optimum at 15:85 mol/mol _mfPA:_dPA. Furthermore, these coatings were shown to bind cells specifically and dependently on αVβ3 integrins (Supplementary Fig. S3). PA coatings above 1.25×10⁻³ M were shown to be toxic to the cells attached in the early stage of culture, inducing cell death and consequent detachment (Fig. 4b). Thus, we hypothesize that the optimal bioactivity found for the 15:85 mol/mol ratio resulted from a balance between the highest epitope density and the lowest epitope crowding effect. Moreover, the susceptibility to degradation by MMPs observed for this binary PA system in solution (Fig. 2) and as a coating (Fig. 3) was not due to the presence of the _dPA as coatings produced solely from _dPA were stable in cSFM (Supplementary Fig. S4). As such, to maximize the interaction between the surface coating and cells, all further experiments were performed using the 15:85 mol/mol _mfPA:_dPA binary system.

OPEN IN VIEWER

FIG. 4.

The adhesive and proliferative bioactivity of _mfPA. (a) The adhesive properties of _mfPA and _mfPA:_dPA coatings allowed human corneal stromal fibroblasts (hCSFs) to attach on low-attachment cell culture plates (upper panels) as in normal culture polystyrene surfaces (lower panels). This effect was dependent on the RGDS motif of _mfPA and not observed in nonbioactive _dPA coatings. Scale bars, 20 μm. (b) Effect of concentration and the _mfPA:_dPA dilution ratio on hCSF proliferation. Cells grown on PA films coating low-attachment surfaces were counted after 7 days in culture. Proliferation increased with increasing PA concentration up to 1.25 mM, after which PA coatings induced cell death. In all viable coatings, maximum proliferative effect was observed for _mfPA:_dPA at the 15:85 mol/mol ratio. No attachment was observed in noncoated (n/c) or _dPA-coated surfaces (0:100 ratio). Data are expressed as average±standard deviation (SD) of three independent experiments (n=3); *** corresponded to p<0.001. Color images available online at www.liebertpub.com/tea

Integrin-mediated cell adhesion has been shown to modulate gene and protein expression and control cell phenotype. ⁴⁰ Previous studies have shown that, when cultured in serum-free media, hCSFs revert to a characteristic alpha smooth muscle actin (αSMA)-negative phenotype and express a defined panel of markers that include keratocan, lumican, decorin, and aldehyde dehydrogenase A1 (ALDH1), all of which are fundamental for the function of the native tissue.^41,42 In this context, the effect of _mfPA:_dPA coating on the expression of several hCSF gene markers was evaluated by reverse transcription quantitative–polymerase chain reaction and compared with that of cells on _dPA-coated and uncoated surfaces (Fig. 5). In this particular assay, normal cell culture polystyrene plates were used to allow cells to adhere and grow, irrespectively of the coating. Importantly, hCSFs grown on bioactive _mfPA:_dPA coatings showed significant upregulation for genes coding for keratocan, lumican, decorin, and ALDH1 (KERA, LUM, DCN, and ALDH1, respectively) compared with cells on nonbioactive _dPA coatings (Fig. 5). However, no effect was observed in the transcription levels of ACTA2 (the gene coding for αSMA, a marker of myofibroblastic differentiation ⁴³ ) or MMP1 (Fig. 5). Furthermore, no differences in gene expression were observed between cells grown on _dPA-coated and uncoated surfaces (data not shown). Together, these results showed that the bioactive PA not only promoted cell adhesion but also directed cell phenotype by enhancing the expression of several specific ECM components without compromising the proven MMP repression effect from RA supplementation to cultured hCSFs. ³³

OPEN IN VIEWER

FIG. 5.

Effect of _mfPA on the molecular phenotype of hCSFs. Relative expression of genes coding for hCSF-characteristic extracellular matrix (ECM) components (KERA, LUM, DCN, and ALDH1) was significantly increased in cells grown for 21 days on _mfPA:_dPA (grey) when compared with _dPA coatings (white bars). Data are expressed as average±SD of three independent experiments (n=3); * and ** corresponded to p<0.05 and 0.01, respectively.

Controlled degradation of PA template and tissue self-release

The potential of _mfPA:_dPA to act as a template capable of inducing the formation of self-releasable tissues under physiological conditions was tested by coating it onto low-attachment culture plates. These plates were subsequently used for the adhesion and long-term culture of hCSFs in serum-free RA-supplemented medium (Fig. 6a). Under these conditions, cells were able to attach, proliferate, and survive for 90 days (Fig. 6b and c) without compromising the adhesive PA surface. The combined effects from _mfPA:_dPA interaction and RA signaling ³³ ensured that cells proliferated up to day 60 (Fig. 6b and c, d60) and stratified from day 30 onward, creating a multilayered construct comprising a cell-derived dense tissue within the boundaries of the PA template (Fig. 6b, d30–d90). Concomitantly, cells maintained in RA-supplemented medium suppressed MMP expression, with corresponding culture supernatants presenting MMP activities below 5% of that from nonsupplemented conditioned medium (Fig. 7). As such, newly formed tissue was cumulatively deposited by cells, and no visible degradation was observed for the entire 90-day period of culture (Fig. 6b). After 90 days in culture, the PA-instructed hCSFs were able to fabricate structurally representative corneal tissue, with an interwoven matrix comprising densely packed collagen fibers and associated native-type proteoglycans, such as keratocan, lumican, and decorin (Fig. 8). These proteoglycans only partially colocalized with the collagen fibers, also occupying the space between the collagenous matrix and the cell layers (Fig. 8, blue staining; also Supplementary Figs S5–S7).

OPEN IN VIEWER

FIG. 6.

Proof of concept for the use of _mfPA as a template to induce the biofabrication and control the self-release of tissues by donor-derived cells. (a) Schematic diagram depicting how cells isolated from human donors were seeded and grown on low-attachment plates coated with 1.25 mM _mfPA:_dPA at the 15:85 mol/mol ratio (green) in retinoic acid (RA)-supplemented SFM (blue). Tissues fabricated in the course of the 90-day culture period were induced to express MMPs into the culture supernatant after RA removal from the medium (orange), thus providing the cue to degrade the adhesive PA coating and induce their self-release (blue arrows). Lifted tissues were then easily recoverable for downstream applications, namely for thrice-repeated reattachment and relifting processes, using only RA-induced regulation of endogenous MMP expression. (b) Phase-contrast micrographs of hCSFs growing as a monolayer on _mfPA:_dPA templates (day [d]7) and integrating the tissue formed by PA-induced ECM deposition and accumulation before (d30–d90), during (+3), and after tissue self-release (R1). Scale bars, 100 μm. (c) Proliferation of hCSFs grown on _mfPA:_dPA templates. Cell proliferation in RA-containing SFM (blue bars) increased up to day 60, and then decreased at day 90 and in the following 3 days, when incubated in SFM without RA (white bars, +1, +2, and +3). Data are expressed as average±SD of three independent experiments (n=3); * and ** corresponded to p<0.05 and 0.01, respectively. Color images available online at www.liebertpub.com/tea

OPEN IN VIEWER

FIG. 7.

MMP activity from cell culture supernatants. (a) Representative casein gel zymogram and (b) corresponding densitometry analysis of supernatant samples collected from 5-day conditioned medium (cSFM) and from various time points where cells were cultured with (blue) or without RA (white bars). Data are expressed as average±SD of three independent experiments (n=3); ** and *** corresponded to p<0.01 and 0.001, respectively. Color images available online at www.liebertpub.com/tea

OPEN IN VIEWER

FIG. 8.

Immunofluorescence confocal microscopy of self-detached tissue constructs. (a) Representative top view and (b) z-stack view of corneal tissues fabricated by hCSFs grown on _mfPA:_dPA templates for 90 days. Tissue constructs showed that they comprise multiple layers of collagen type I fibers (red) deposited between actin-stained cells (green) and form an ECM enriched with hCSF-characteristic proteoglycans (blue), such as keratocan (left), lumican (center), and decorin (right panels). Scale bars, 20 μm. Color images available online at www.liebertpub.com/tea

Upon formation of the biofabricated constructs at day 90, tissue cultures were then maintained in the medium without RA (Fig. 6a), and MMP proteolytic activity was monitored each day for 3 days (days +1, +2, and +3). Removal of RA supplementation resulted in a cumulative increase of proteolytic activity in the culture supernatant over time (Fig. 7), a consequence of MMPs being expressed from cells within the tissue constructs. Although MMP activity at day +3 corresponded to only 45%±13% of that from conditioned medium, it allowed the newly formed tissues to completely detach from the surface of the culture plate and emerge (Fig. 6a and Supplementary Video S1). This indicated that, by day +3, the MMP activity accumulated in the medium was sufficient to cleave the RGDS motif from the _mfPA:_dPA nanotapes and degrade the adhesive coating template (Fig. 6a). The subsequent self-release and liftoff of the tissue at day +3 resulted in a small, but significant, loss of cells (Fig. 6c). However, the free-floating construct maintained its structural integrity (Fig. 9 and Supplementary Video S2), with no apparent loss of the dense ECM present at d90 (Fig. 6b, +3).

OPEN IN VIEWER

FIG. 9.

Photograph of free-floating, human corneal tissue fabricated by hSCFs. Tissue constructs fabricated by cells during 90 days recovered their ability to express MMPs after being maintained in SFM without RA. The endogenous MMPs expressed by the tissue were capable of degrading the underlying _mfPA:_dPA template after 3 days, thus inducing the tissue self-release from its adhesive bottom surface without any external manipulation. The resulting free-floating tissue was transparent in water and retained its original shape and structural integrity. A decimal ruler was used as scale.

Similarly, skin fibroblasts seeded onto the PA surface templates and cultured for 90 days in RA-supplemented SFM were able to attach, proliferate, and fabricate ECM-dense three-dimensional tissues. Following the same method of RA removal, the newly formed skin tissues were able to express endogenous MMPs, thus degrading the adhesive PA template and promoting their self-detachment while retaining structural integrity and shape (Supplementary Video S3). These results indicated that more than one cell type can be applied to this PA system and to produce easily recoverable structurally intact tissues through the physiologically relevant RA supplementation/removal mechanism. However, for cell types where RA is not a feasible regulator of endogenous MMP expression, alternative systems could be used with predictably similar results. For example, alternative inhibitors of MMP expression (such as the anticancer agents Marimastat, ⁴⁴ Ro 28-2653, ⁴⁵ and doxycycline ⁴⁶ ) or enzymatic activity (e.g., tissue inhibitors of metalloproteinase or α₂-macroglobulin ⁴⁷ ) could be added throughout the culture process, and then removed to transiently increase MMP activity and elicit the degradation of the PA template and tissue release.

Functional characterization of self-released tissue equivalents

To further characterize the efficacy of the self-release process, the viability, ultrastructure, and function of the self-lifted tissues were analyzed. Tissue function and viability were tested by determining the capacity of the corneal stromal constructs to reattach to an adhesive surface after MMP-induced self-release while maintaining their constituent cells alive. For this, first-time self-released tissues (R1) were punch-sampled to assess cell viability through a live/dead cell assay, reconditioned in RA-containing medium for 3 days to suppress MMP expression, and then transferred to a new _mfPA:_dPA-coated, low-attachment cell culture plate for reattachment (Fig. 6a). After 7 days, tissue constructs were shown to be planar and strongly attached to _mfPA:_dPA-coated surfaces (Fig. 10a, R1), but not to uncoated controls where they remained free-floating (Fig. 10a, uncoated). Furthermore, the viability of cells comprising the tissue was maintained with 92%±1% of live cells (Fig. 10b and c, R1). The ultrastructure of R1 corneal stromal tissues was evaluated by TEM (Fig. 10d). These 10.1±1.8-μm-thick bioconstructs comprised two or three cells layers separated by 2.6±1.6-μm-thick regions of ECM (Fig. 10d, upper panel) characterized by densely packed collagen fibrils with an average diameter of 27.6±2.8 nm and a d-spacing of 58.5±4.5 nm (Fig. 10d, lower panels). Interestingly, the ECM thickness of these tissues was similar to that of stromal lamellae (i.e., the building blocks) of native human corneas. ⁴⁸ Moreover, the calculated diameter and d-spacing parameters were comparable with those of collagen type I fibers found in the human cornea,^48,49 representing an improvement over similar tissue constructs produced de novo by diverse methods.^50,51 These results were consistent with those obtained by confocal immunofluorescence microscopy (Fig. 8) and constituted proof that the tissue fabric produced in vitro by hCSFs grown in _mfPA:_dPA-coated surfaces was remarkably similar to that of the native corneal stroma.

OPEN IN VIEWER

FIG. 10.

Macro-, micro-, and ultrastructural stability of tissues fabricated and self-released from PA templates. (a) Reattachment of biofabricated tissues after one (R1), two (R2), or three consecutive self-detachments (R3) to low-attachment plates with or without (uncoated) _mfPA:_dPA. Self-released tissues were punch-sampled and reconditioned in RA-supplemented SFM before being transferred for reattachment (Fig. 6a). Scale bars, 3 mm. (b) Live and dead hCSFs integrating the self-released tissues imaged by fluorescence microscopy using calcein (green)/propidium iodide (red) cell staining (Scale bars, 20 μm), and (c) corresponding cell viability was calculated for R1, R2, and R3 tissue constructs. Data are expressed as average±SD of three independent experiments (n=3); ** corresponded to p<0.01. (d) Ultrastructure of R1 was observed by transmission electron microscopy, evidencing the multilayered organization of the tissue (upper panel), with intercalating layers of hCSFs and deposited ECM comprising densely packed collagen fibrils (lower panels). Scale bars, 500 nm (upper), 100 nm (lower panels). Color images available online at www.liebertpub.com/tea

The endurance of the tissue constructs was further tested by repeating the relift, punching, and reattachment processes two additional times, consecutively (Fig. 6a). Second-time released tissues (R2) were always able to reattach to coated surfaces while maintaining shape throughout the manipulation process (Fig. 10a). In addition, cell viability remained high with R2 tissues maintaining 85%±13% of live cells (Fig. 10b, c), representing maintenance of cell viability within the tissue for 13 days following initial release (Fig. 6a). Reattachment was even observed after three consecutive self-releases (Fig. 10a, R3), despite evidence of some structural damage and a significant reduction in cell viability to 40%±2% (Fig. 10c). These results demonstrate that the fabricated tissue constructs were very robust, being able to respond to RA deprivation, control their MMP-mediated self-release, and then be RA reconditioned and reattach to an adhesive surface three consecutive times. In addition, tissues remained structurally whole and morphologically intact despite their thickness and the endogenous MMPs eliciting tissue self-release. These results also suggested that self-released tissue constructs could perform well as live transplantable equivalents. Cell sheet constructs used as corneal, ⁵² esophageal, ⁵³ tracheal, ⁵⁴ myocardial, ⁵⁵ and hepatic ⁵⁶ implants have previously shown improved practical and functional advantage due to their intrinsic ability to attach to the recipient organ without the need for sutures or carrier materials, leading to quicker graft integration and organ regeneration. ⁵⁷ As such, the demonstrated ability of the self-released tissues to reattach to adhesive surfaces represents a promising clinical application.

The specific function of cells within the released tissues was also evaluated. It is known that hCSFs play a fundamental role in response to corneal injury in vivo by assuming a contractile myofibroblastic phenotype. ⁴³ This cellular function was simulated here by exposing free-floating, corneal stromal tissues after self-release to FBS for 7 days (Fig. 11a). The results showed that R1 tissues contracted to 8%±3% of their initial area (Fig. 11b), indicating that cells comprising the tissue constructs were activated and contractile. The hCSFs comprising second- (R2) and third-time self-released tissues (R3) were also prone to activation, as demonstrated by tissue contraction to a corresponding 9%±5% and 32%±5% of their initial size (Fig. 11). The reduced contraction from R3 could be explained by the lower number of resident hCSFs within those tissues (Fig. 10c). However, the cells remaining alive were still capable of performing a change in phenotype and activating into myofibroblasts. Together, these results showed that tissue fabricated in vitro by hCSFs grown on _mfPA:_dPA templates were structurally and functionally similar to native stromal tissue from human corneas and that the controlled self-release resulting from the specific degradation of the adhesive _mfPA:_dPA coating allowed an easy tissue recovery (Supplementary Video S2) with minimal damage to its cells or ECM structure. Furthermore, even after extensive manipulation, the tissues were able to reattach and relift three consecutive times while remaining functionally active for an additional 26-day period in culture, as demonstrated by the contraction assay.

OPEN IN VIEWER

FIG. 11.

Biofunctional performance of tissues fabricated and self-released from PA templates. Myofibroblastic activity of hCSFs from self-released R1, R2, and R3 tissues after incubation in fetal bovine serum (FBS)-containing medium. (a) Representative photographs taken before (day 0, upper half) and after FBS incubation (day 7, lower half) were overlapped to evidence reduction in the tissue area. Scale bars, 5 mm. (b) Quantification of tissue contraction expressed as a ratio between the final and initial areas of tissue. Data are expressed as average±SD of three independent experiments (n=3); ** corresponded to p<0.01.