Outcome of Large Noninvasive Follicular Thyroid Neoplasm with Papillary-Like Nuclear Features

Abstract

Background:

In 2016, encapsulated follicular variant of papillary thyroid carcinoma without invasion was renamed “noninvasive follicular thyroid neoplasm with papillary-like nuclear features” (NIFTP) in order to reduce overtreatment of this indolent tumor. However, many endocrinologists remain uneasy about managing large (≥4 cm) NIFTP conservatively without radioactive iodine (RAI) therapy. The objectives of this study are to characterize the clinicopathologic characteristics and outcome of large NIFTP in order to assist therapeutic decision making.

Methods:

The pathology databases of four tertiary hospitals were searched for large (≥4 cm) NIFTP. Cases with separate foci of carcinoma were excluded. Seventy-nine cases fulfilled the inclusion criteria. Among them, 56 (71%) had at least two years of clinical follow-up (FU), and 49 (62%) had four or more years of FU. The clinicopathologic characteristics were reviewed and documented by four endocrine pathologists.

Results:

The median size of the NIFTP was 4.5 cm (range 4.0–8.0 cm). The entire capsule was sampled in 50 (63%) tumors, while in the remaining 29 (37%) cases, it was submitted representatively, with a median of 2.1 blocks per centimeter of tumor examined. Large NIFTP had a female preponderance with a male:female ratio of 1:1.8, and presented at a median age of 49 years. There were no lymph node metastases at diagnosis in any of the patients, and none of the patients (n = 25) in whom nodal tissue was available for microscopic examination had positive findings. Twenty-six (33%) underwent thyroid lobectomy alone, and 37 (47%) did not receive RAI ablation. No recurrence was observed in the entire cohort, including all 32 patients with two or more years of FU who did not receive RAI therapy (median FU: 6.7 years). Among patients with four or more years of FU, all 25 individuals without RAI therapy did not recur, with a median FU of 11.2 years. Patients with a larger tumor size tended to receive postoperative RAI ablation (p = 0.001).

Conclusions:

Similar to their small counterparts, large NIFTP appear to have an extremely low risk of recurrence (zero in this cohort), even when treated conservatively without RAI therapy. Surgical treatment alone, including lobectomy, appears to be adequate for large NIFTP.

Introduction

Thyroid carcinoma is the cancer with the largest increase of incidence in the United States in the past decade, with 62,980 new cases diagnosed annually (1). This increase is mostly attributed to a sharp rise in follicular variant of papillary thyroid carcinomas (FVPTC) and papillary microcarcinomas (2). Regardless of tumor size, the proportion of papillary carcinomas with follicular growth pattern (i.e., FVPTC and follicular variants of papillary microcarcinomas) among all papillary thyroid carcinomas (PTC) has tripled from 18% to 57% in the past four decades, surpassing the papillary (classical or usual) pattern and becoming the most common architectural pattern encountered in PTC (2).

Histologically, FVPTC is characterized by an almost exclusive follicular growth pattern and the presence of nuclear features of PTC, for example nuclear enlargement, nuclear membrane irregularity, and chromatin clearing and margination (3,4). Recent molecular studies, including The Cancer Genomic Atlas (TCGA) of PTC, have shown that FVPTC are often RAS mutated and carry a molecular signature resembling the follicular adenoma (FA)/follicular carcinoma group of tumors and are distinctly different from the classical variant of PTC that are often BRAF mutated (5 –7). The encapsulated noninvasive form of FVPTC in particular has been shown to follow an extremely indolent clinical course, with a negligible risk of lymph node metastasis and recurrence when completely resected (6). Realizing the epidemic of diagnoses, unique molecular signatures, and highly indolent nature of the encapsulated noninvasive FVPTC, a working group of 28 internationally renowned endocrine experts critically reexamined this entity in 2014 (3). Among the 108 cases of encapsulated noninvasive FVPTC with no radioactive iodine (RAI) treatment and with at least 10 years of follow-up (FU), there was not a single recurrence. Hence, the consensus group advocated for a nomenclature revision of this entity to “noninvasive follicular thyroid neoplasm with papillary-like nuclear features” (NIFTP), in an effort to reduce overtreatment by eliminating the term “carcinoma” (3).

Prior to the introduction of the NIFTP term, large encapsulated noninvasive FVPTC were staged as pT3 and clinical stage III in patients >45 years of age using the 7th edition of the American Joint Committee on Cancer (AJCC) staging manual (8). According to the 2015 American Thyroid Association (ATA) guidelines, postoperative RAI should be considered in these lesions (9). Hence, many endocrinologists remain uneasy about managing large NIFTP ≥4 cm in size conservatively without postoperative RAI scanning and/or ablation.

In order to shed light on the prognosis of and appropriate treatment options for large NIFTP, a large cohort of 79 cases of NIFTP ≥4 cm in size from four tertiary hospitals was studied.

Material and Methods

Study cohort

After obtaining approval from the various Institutional Review Boards, the pathology databases of four tertiary hospitals (Sunnybrook Health Sciences Centre [SHSC], Toronto, Canada; Ospedale Maggiore, Bologna, Italy; Cornell University, New York, NY; and Memorial Sloan Kettering Cancer Center [MSKCC], New York, NY) were searched. The search periods and criteria were: SHSC 1999–2015, FVPTC and FA; Ospedale Maggiore 1995–2010, FA; Cornell University 2000–2014, FVPTC; and MSKCC 1982–2015, FVPTC. Each of the four pathologists (B.X., G.T., T.S., and R.G.) independently reviewed all the slides from each case emanating from his/her institution to confirm the diagnosis of NIFTP using the criteria proposed by Nikiforov et al. (3). In brief, NIFTP was diagnosed when all of the following criteria were met: (i) encapsulation or clear demarcation; (ii) exclusive/predominant follicular growth pattern lacking psammoma bodies and with <1% true papillae and 30% solid growth pattern; (iii) nuclear atypia in the form of nuclear enlargement, nuclear membrane irregularity, and/or chromatin clearing with a nuclear score of 2–3; (iv) absence of invasion (vascular or capsular); (v) no tumor necrosis; and (vi) a mitotic index <3 per 10 high power fields (400 × ; Fig. 1). In regard to the scoring system developed in the original NIFTP paper (3), the nuclear features of the tumor were scored based on three criteria: (i) size and shape (nuclear enlargement, overlapping, and/or elongation), (ii) nuclear membrane irregularities (irregular contours, grooves, and/or pseudo-inclusions), and (iii) chromatin characteristics (chromatin clearing, margination of chromatin to membrane, and/or glassy nuclei). A three-point scoring scheme was developed, in which each class of nuclear features was assigned a score of 0 or 1, yielding a range of scores from 0 to 3. A diagnostic score of 2 or 3 was required for a diagnosis of NIFTP. The extent of PTC nuclei in the entire lesion was not studied, since the original NIFTP study did not require a specific minimal percentage of the tumor to demonstrate these nuclear features. Capsular and vascular invasion was diagnosed according to the Armed Forces Institute of Pathology (AFIP) atlas on tumors of the thyroid gland (10). Capsular invasion was defined as complete penetration of the lesional capsule by a tumor bud. The presence of vascular invasion was noted only when such foci were present within or beyond the tumor capsule. Briefly, only when the invasive focus protruded into the lumen of the vessel in a polypoid manner covered by endothelial cells, or when it was attached to the vessel wall or associated with thrombus formation, was it considered true vascular invasion. Cases with separate foci of carcinoma (mostly papillary microcarcinomas) were excluded from the study. A total of 79 cases fulfilling the above criteria were included in the present study.

FIG. 1.

Histology pictures of a 5.6 cm noninvasive follicular thyroid neoplasm with papillary-like nuclear features (NIFTP) treated by lobectomy without radioactive iodine therapy in a 65-year-old male patient. The patient was alive without evidence of disease 13 years and 4 months after diagnosis. Microscopic pictures of the tumor in (A) at low power (40 × ) show an encapsulated follicular noninvasive neoplasm (arrow, tumor capsule; tu, tumor; Nl, normal adjacent thyroid). In (B), at high power (600 × ), NIFTP is composed of follicles lined by enlarged, clear, irregular, overlapping nuclei with grooves (arrows).

Clinical review

Each patient's chart was reviewed to record the following clinical parameters: age at diagnosis, sex, type of resection (total thyroidectomy vs. lobectomy/hemithyroidectomy), status of postoperative RAI ablation, duration of clinical FU, and clinical outcome.

Statistics

All statistical analyses were performed using IBM SPSS Statistics for Windows v22.0 (IBM Corp., Armonk, NY). The association between different clinicopathologic characteristics and RAI status was calculated using Fisher's exact test for categorical variables and a two-tailed Student's t-test for continuous variables. p-Values of <0.05 were considered to be statistically significant.

Results

Seventy-nine patients fulfilling the inclusion criteria were included in this study. Thirteen of these cases have been previously reported as NIFTP (3). The numbers of cases according to the institutions were: MSKCC N = 56; SHSC N = 11; Bologna University N = 7; and Cornell University N = 5. The clinicopathologic characteristics are summarized in Table 1. Large NIFTP were more frequently present in female patients, with a male to female ratio of 1:1.8. The median age at diagnosis was 49 years (range 22–81 years). The median size of the NIFTP was 4.5 cm (range 4.0–8.0 cm). All tumors were confined to the thyroid and were resected completely with negative surgical margins during the initial surgery. There were no lymph node metastases at diagnosis in any of the patients, and none of the patients (n = 25) in whom nodal tissue was available for microscopic examination had positive findings.

Table 1.

Clinicopathologic Characteristics of Patients with Large NIFTP ^a

All cases (n = 79)
Sex
Female	51	65%
Male	28	35%
Age, years, median (range)	49 (22–81)
Size, cm, median (range)	4.5 (4.0–8.0)
Sampling of NIFTP
Entirely or entire capsule	50	63%
Representative	29	37%
Blocks sampled per cm of tumor if only representatively sampled, median (range)	2.1 (0.5–4.2)
Sampling and status of lymph nodes
Not sampled	54	68%
Benign lymph node(s)	25	32%
Surgical procedure
Lobectomy	26	33%
Total/subtotal thyroidectomy	53	67%
Postoperative RAI ablation
Not available	15	19%
No RAI	37	47%
RAI	27	34%
FU duration, years, median, mean (range)	5.8, 7.3 (0.3–26.0)
Disease status at last FU
Not available	15	19%
NED	64	81%
Patients with large NIFTP and at least 2-year FU (n = 60)
RAI
Not available	1	2%
No RAI	32	53%
RAI	27	45%
Surgical procedure
Lobectomy	17	28%
Total/subtotal thyroidectomy	43	72%
FU duration, years, median. mean (range)	5.9, 7.7 (2.0–26.0)
Disease status at last FU
NED	60	100%
Patients with large NIFTP and at least 4-year FU (n = 49)
RAI
Not available	1	2%
No RAI	25	51%
RAI	23	47%
Surgical procedure
Lobectomy	15	31%
Total/subtotal thyroidectomy	34	69%
FU duration, years, median, mean (range)	7.2, 8.8 (4.0–26.0)
Disease status at last FU
NED	49	100%

All values are expressed as n and % of column total unless otherwise specified.

NIFTP, noninvasive follicular thyroid neoplasm with papillary-like nuclear features; RAI, radioactive iodine; FU, follow-up; NED, no evidence of disease.

The entire tumor capsule or the entire tumor was examined histologically in 50 (63%) cases, while in the remaining 29 (37%), the tumor was submitted representatively with a median of 2.1 blocks (range 0.5–4.2) per centimeter of tumor examined.

Among these 79 patients, 64 (81%) had clinical FU available, with a median FU duration of 5.8 years (M = 7.3 years; range 0.3–26.0 years). Sixty (76%) had at least two years, and 49 (62%) had at least four years of FU. Twenty-six (33%) patients underwent thyroid lobectomy for their primary tumor, while the remaining patients were treated with total/subtotal thyroidectomy. Thirty-seven (47%) did not receive postoperative RAI ablation. No recurrence or disease-specific death was observed in the entire cohort, including all 32 patients with two or more years of FU who did not receive RAI therapy (median FU in this group of patients: 6.7 years; M = 8.6 years). Among patients with four or more years of FU, all 25 individuals without RAI therapy did not recur, with a median FU of 11.2 years (M = 10.1 years).

In this cohort, it appeared that patients with a larger size of NIFTP were treated significantly more frequently with RAI (p = 0.001; Table 2). The median size of the NIFTP in the group subjected to RAI was 5.0 cm compared with 4.2 cm in those patients who did not receive RAI. Other parameters, for example age and sex, did not have a significant impact on RAI administration in this cohort. As total thyroidectomy was a prerequisite for postoperative RAI, it was not surprising to detect a significant correlation between the type of surgical procedure and RAI ablation (p < 0.001).

Table 2.

RAI Status in Patients with Large NIFTP and At Least Two- or Four-Year FU ^a

	No RAI		RAI		p-Value
Patients with at least two-year FU (n = 59)
n	32		27
Sex
Female	23	72%	17	63%	0.579
Male	9	28%	10	37%
Surgical procedure
Lobectomy	17	53%	0	0%	<0.001
Total/subtotal thyroidectomy	15	47%	27	100%
Sampling of NIFTP
Entirely/entire capsule	20	63%	14	52%	0.440
Representative	12	38%	13	48%
Sampling and status of lymph nodes
Not sampled	24	75%	18	67%	0.569
Benign lymph node(s)	8	25%	9	33%
Age, years, median (range)	50 (27–76)		49 (24–64)		0.517
Size, cm, median (range)	4.2 (4.0–5.8)		5.0 (4.0–7.5)		0.001
FU duration, years, median, mean (range)	7.5, 7.5 (2.4–20.5)		5.8, 6.8 (2.0–26.0)		0.181
Blocks sampled per cm of tumor if only representatively sampled, median (range)	2.2 (0.8–4.1)		2.0 (0.5–4.2)		0.614
Patients with at least four-year FU (n = 48)
n	25	100%	23	100%
Surgical procedure
Lobectomy	15	60%	0	0%	<0.001
Total/subtotal thyroidectomy	10	40%	23	100%
Size, cm, median (range)	4.2 (4.0–5.6)		5.0 (4.0–7.5)		0.001

All values are expressed as n and % of column total unless otherwise specified.

p-Values are calculated using Fisher's exact test for categorical variables and a two-tailed Student's t-test for continuous variables.

Discussion

Encapsulated/well-demarcated noninvasive FVPTC has an estimated annual occurrence of 45,000 worldwide and was considered a cancer for the past three decades (11). It is not until recently that compelling molecular evidence and clinical outcome data have demonstrated that noninvasive encapsulated FVPTC follows a highly indolent clinical course with negligible risk of recurrence, and it carries a molecular signature resembling FA/follicular carcinoma with RAS mutations as the most frequently encountered alteration (3,5 –7,12). In 2016, the “group for the re-examination of the encapsulated follicular variant of papillary thyroid cancer” studied 106 patients with noninvasive encapsulated FVPTC with at least 10 years of FU and provided an in-depth analysis of the morphological, molecular, and clinical findings of these lesions (3). Based on the negligible recurrence risk of this lesion, the group proposed a nomenclature change to NIFTP, eliminating the word “carcinoma” (3). This modification in the diagnostic terminology aimed at reducing overtreatment and eliminating the social stigma associated with a cancer diagnosis. Nevertheless, many clinicians remain uncomfortable treating a patient with large NIFTP ≥4 cm in size conservatively, as these lesions used to be staged as T3 and stage III (if >45 years old) using the AJCC staging manual 7th edition (8). This discomfort is confirmed by data from the present study in which patients with larger tumors were treated significantly more frequently with RAI. To date, no studies have focused on the prognosis of large (≥4 cm) NIFTP, previously termed noninvasive encapsulated follicular variant of PTC.

None of the patients in the present cohort harboring large NIFTP had lymph node metastasis or disease recurrence, including those patients treated with surgery alone without postoperative RAI. The data provide additional confirmatory evidence to previous reports demonstrating the highly indolent nature of NIFTP in general. Although not centered on large NIFTP, a few of these previous publications included some large NIFTP (up to 9 cm) that did not recur, which is in line with the findings of the current series (3,13,14).

In the English language literature, only 2/388 (0.5%) well-documented, well-circumscribed/encapsulated noninvasive FVPTC (now renamed as NIFTP) recurred (13,15 –21). Of note, the tumor was present at the margin in one of the relapsing cases, and the noninvasive nature of the tumor is questionable in the second case (3). Even if these two relapsing tumors were included, the recurrence rate of these tumors still remains extremely low (<0.5%) based on the total number of patients published and the current series. In order to have a pure population of NIFTP, four endocrine pathologists with special interests in thyroid neoplasia reviewed these tumors, applying the stringent criteria advocated for the diagnosis of this entity (3). A meticulous histopathologic examination is of paramount importance, since a deviation from these criteria may affect outcomes. For example, the presence of a minor but significant amount of papillae formation (>1%) may increase the risk of lymph node metastasis. It was previously reported that encapsulated carcinomas harboring >1% papillae (encapsulated classical PTC) can give rise to lymph node metastasis, despite a total lack of invasion in 27% of cases (22). Evidently, the most crucial parameter for the diagnosis of NIFTP is the evaluation of capsular and vascular invasion. The definition of invasion can vary between pathologists and constitute a source of inter-observer variability. Therefore, the most widely utilized and validated definitions for capsular/vascular invasion were used. The latter are clearly described in the most recent authoritative AFIP atlas on tumors of the thyroid gland (10). Another requirement for an accurate assessment of invasion is a well-sampled tumor capsule. In the current series, the entire capsule was microscopically examined in 50 (63%) of the tumors, while in the remaining 29 (37%) cases, it was submitted representatively, with a median of 2.1 blocks per centimeter of tumor examined. This is clearly above the required one block of tumor capsule per centimeter of tumor recommended by the AFIP atlas on tumors of the thyroid gland (10). It is therefore thought that the present cases met all the required criteria for NIFTP, including adequate sampling. Indeed, in the original NIFTP paper, the authors stated that adequacy of tumor capsule microscopic examination is imperative for the diagnosis of NIFTP (3). They further commented that “adequacy of tumor capsule sampling has not been discussed in the literature to date with respect to FVPTC.” They and the current authors believe that sampling of the entire tumor capsule is preferable. Nikiforov et al. concluded by saying that “NIFTP should undergo extensive review of the tumor capsule interface to exclude invasion” (3) but stay short of requiring examination of the entire tumor capsule in order to diagnose NIFTP. To understand the biologic behavior of uninodular large NIFTP independently from the presence of separate carcinomas in the thyroid, all cases harboring additional foci of carcinoma were excluded. In view of that design, how often NIFTP is part of a multicentric tumor process could not be assessed. In regard to FU, there was a reliance on structural rather than biochemical recurrence to assess a patient's disease status. This was in part due to the fact that some archived cases were not evaluated recently and did not have adequate serum thyroglobulin data. Although a long FU time was not available in all cases, this study comprised 25 patients treated by surgery without RAI therapy who did not recur, with a median FU of 11.2 years. These data strongly suggest that large NIFTP have an extremely low relapse rate. Indeed, most differentiated thyroid carcinomas recur during the first decade (23), although late recurrences and distant spread have been documented (24). The lack of recurrence in these patients without adjuvant RAI therapy suggests that they could be spared completion thyroidectomy and RAI, despite the large size of their tumors. These patients could then avoid the potential complications of total thyroidectomy, eventual side effects of RAI therapy, and the financial costs associated with these therapeutic modalities (25,26). The indolent behavior of these large NIFTP further reinforces the fact that invasion rather than nuclear features or tumor size drives outcomes in encapsulated follicular-patterned tumors (18). The lack of nodal metastasis at presentation in all cases, including those with microscopically examined lymph nodes, is also consistent with the fact that these are mainly RAS- as well as PAX8-PPARG-driven neoplasms and hence have a behavior very similar to FAs (12). Indeed, encapsulated RAS-driven follicular cell–derived thyroid tumors (e.g., follicular carcinomas, NIFTP, encapsulated FVPTC with invasion) do not usually spread to lymph nodes in sharp contrast to BRAF-mutated classical PTC (10,22).

In conclusion, this study suggests that large NIFTP have an extremely low risk of recurrence, even when treated conservatively without RAI therapy. Conservative surgical treatment alone appears to be adequate for large NIFTP. Future long-term follow-up studies on large NIFTP will help confirm these findings and further reassure patients and their clinicians.

Footnotes

Acknowledgments

Research reported in this publication was supported in part by the Cancer Center Support Grant of the National Institutes of Health/National Cancer Institute under award number P30CA008748. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.

Author Disclosure Statement

No competing financial interests exist for all contributory authors.

References

Siegel

, Ma

, Zou

, Jemal

. 2014. Cancer statistics, 2014. CA Cancer J Clin, 64:9–29.

Jung

, Little

, Lubin

, Brenner

, Wells

Jr , Sigurdson

, Nikiforov

. 2014. The increase in thyroid cancer incidence during the last four decades is accompanied by a high frequency of BRAF mutations and a sharp increase in RAS mutations. J Clin Endocrinol Metab, 99:E276–285.

Nikiforov

, Seethala

, Tallini

, Baloch

, Basolo

, Thompson

, Barletta

, Wenig

, Al Ghuzlan

, Kakudo

, Giordano

, Alves

, Khanafshar

, Asa

, El-Naggar

, Gooding

, Hodak

, Lloyd

, Maytal

, Mete

, Nikiforova

, Nose

, Papotti

, Poller

, Sadow

, Tischler

, Tuttle

, Wall

, LiVolsi

, Randolph

, Ghossein

. 2016. Nomenclature revision for encapsulated follicular variant of papillary thyroid carcinoma: a paradigm shift to reduce overtreatment of indolent tumors. JAMA Oncol, 2:1023–1029.

DeLellis

, Lloyd

, Heitz

, Eng

. 2004. Pathology and Genetics of Tumours of the Endocrine Organs. IARC Press, Lyon, France.

Agrawal

, Akbani

, Aksoy

, Ally

, Arachchi

, Asa

, Auman

, Balasundaram

, Balu

, Baylin

, Behera

, Bernard

, Beroukhim

, Bishop

, Black

, Bodenheimer

, Boice

, Bootwalla

, Bowen

, Bowlby

, Bristow

, Brookens

, Brooks

, Bryant

, Buda

, Butterfield

YSN

, Carling

, Carlsen

, Carter

, Carty

, Chan

, Chen

, Cherniack

, Cheung

, Chin

, Cho

, Chu

, Chuah

, Cibulskis

, Ciriello

, Clarke

, Clayman

, Cope

, Copland

, Covington

, Danilova

, Davidsen

, Demchok

, DiCara

, Dhalla

, Dhir

, Dookran

, Dresdner

, Eldridge

, Eley

, El-Naggar

, Eng

, Fagin

, Fennell

, Ferris

, Fisher

, Frazer

, Frick

, Gabriel

, Ganly

, Gao

, Garraway

, Gastier-Foster

, Getz

, Gehlenborg

, Ghossein

, Gibbs

, Giordano

, Gomez-Hernandez

, Grimsby

, Gross

, Guin

, Hadjipanayis

, Harper

, Hayes

, Heiman

, Herman

, Hoadley

, Hofree

, Holt

, Hoyle

, Huang

, Hutter

, Ideker

, Iype

, Jacobsen

, Jefferys

, Jones

SJM

, Kasaian

, Kebebew

, Khuri

, Kim

, Kramer

, Kreisberg

, Kucherlapati

, Kwiatkowski

, Ladanyi

, Lai

, Laird

, Lander

, Lawrence

, Lee

, Leraas

, Lichtenberg

, Lichtenstein

, Lin

, Ling

, Liu

, LiVolsi

, Lu

, Ma

, Mahadeshwar

, Marra

, Mayo

, McFadden

, Meng

, Meyerson

, Mieczkowski

, Miller

, Mills

, Moore

, Mose

, Mungall

, Murray

, Nikiforov

, Noble

, Ojesina

, Owonikoko

, Ozenberger

, Pantazi

, Parfenov

, Park

, Parker

, Paull

, Pedamallu

, Perou

, Prins

, Protopopov

, Ramalingam

, Ramirez

, Raphael

, Rathmell

, Ren

, Reynolds

, Rheinbay

, Ringel

, Rivera

, Roach

, Robertson

, Rosenberg

, Rosenthall

, Sadeghi

, Saksena

, Sander

, Santoso

, Schein

, Schultz

, Schumacher

, Seethala

, Seidman

, Senbabaoglu

, Seth

, Sharpe

, Mills Shaw

, Shen

, Sherman

, Sheth

, Shi

, Shmulevich

, Sica

, Simons

, Sipahimalani

, Smallridge

, Sofia

, Soloway

, Song

, Sougnez

, Stewart

, Stojanov

, Stuart

, Tabak

, Tam

, Tan

, Tang

, Tarnuzzer

, Taylor

, Thiessen

, Thorne

, Thorsson

, Tuttle

, Umbricht

, Van Den Berg

, Vandin

, Veluvolu

, Verhaak

RGW

, Vinco

, Voet

, Walter

, Wang

, Waring

, Weinberger

, Weinstein

, Weisenberger

, Wheeler

, Wilkerson

, Wilson

, Williams

, Winer

, Wise

, Wu

, Xi

, Xu

, Yang

, Zack

, Zeiger

, Zeng

, Zenklusen

, Zhao

, Zhang

, Zmuda

, Zou

. 2014. Integrated genomic characterization of papillary thyroid carcinoma. Cell, 159:676–690.

Liu

, Singh

, Tallini

, Carlson

, Katabi

, Shaha

, Tuttle

, Ghossein

. 2006. Follicular variant of papillary thyroid carcinoma: a clinicopathologic study of a problematic entity. Cancer, 107:1255–1264.

Zhu

, Gandhi

, Nikiforova

, Fischer

, Nikiforov

. 2003. Molecular profile and clinical-pathologic features of the follicular variant of papillary thyroid carcinoma. An unusually high prevalence of ras mutations. Am J Clin Pathol, 120:71–77.

Edge

, Byrd

, Compton

, Fritz

, Greene

, Torotti

. 2010. AJCC Cancer Staging Manual. Seventh edition. Springer-Verlag, New York, NY.

Haugen

BRM

, Alexander

, Bible

, Doherty

, Mandel

, Nikiforov

, Pacini

, Randolph

, Sawka

, Schlumberger

, Schuff

, Sherman

, Sosa

, Steward

, Tuttle

RMM

, Wartofsky

. 2016. 2015 American Thyroid Association management guidelines for adult patients with thyroid nodules and differentiated thyroid cancer. Thyroid, 26:1–133.

10.

Rosai

, DeLellis

, Carcangiu

, Frable

, Tallini

. 2015. Tumor of the Thyroid and Parathyroid Gland (AFIP Atlas of Tumor Pathology Series 4). American Registry of Pathology Press, Silver Spring, MD.

11.

Tallini

, Tuttle

, Ghossein

. 2016. The history of the follicular variant of papillary thyroid carcinoma. J Clin Endocrinol Metab [Epub ahead of print]; DOI: 10.1210/jc.2016-2976.

12.

Rivera

, Ricarte-Filho

, Knauf

, Shaha

, Tuttle

, Fagin

, Ghossein

. 2010. Molecular genotyping of papillary thyroid carcinoma follicular variant according to its histological subtypes (encapsulated vs infiltrative) reveals distinct BRAF and RAS mutation patterns. Mod Pathol, 23:1191–1200.

13.

Howitt

, Paulson

, Barletta

. 2015. Absence of BRAF V600E in non-infiltrative, non-invasive follicular variant of papillary thyroid carcinoma. Histopathology, 67:579–582.

14.

Rosario

, Mourao

, Nunes

, Calsolari

. 2016. Noninvasive follicular thyroid neoplasm with papillary-like nuclear features. Endocr Relat Cancer, 23:893–897.

15.

Thompson

. 2016. Ninety-four cases of encapsulated follicular variant of papillary thyroid carcinoma: a name change to noninvasive follicular thyroid neoplasm with papillary-like nuclear features would help prevent overtreatment. Mod Pathol, 29:698–707.

16.

Piana

, Frasoldati

, Di Felice

, Gardini

, Tallini

, Rosai

. 2010. Encapsulated well-differentiated follicular-patterned thyroid carcinomas do not play a significant role in the fatality rates from thyroid carcinoma. Am J Surg Pathol, 34:868–872.

17.

Vivero

, Kraft

, Barletta

. 2013. Risk stratification of follicular variant of papillary thyroid carcinoma. Thyroid, 23:273–279.

18.

Ganly

, Wang

, Tuttle

, Katabi

, Ceballos

, Harach

, Ghossein

. 2015. Invasion rather than nuclear features correlates with outcome in encapsulated follicular tumors: further evidence for the reclassification of the encapsulated papillary thyroid carcinoma follicular variant. Hum Pathol, 46:657–664.

19.

Rosario

, Penna

, Calsolari

. 2014. Noninvasive encapsulated follicular variant of papillary thyroid carcinoma: is lobectomy sufficient for tumours ≥1 cm?. Clin Endocrinol (Oxf), 81:630–632.

20.

Liu

, Zhou

, Nakamura

, Koike

, Li

, Ozaki

, Mori

, Taniguchi

, Kakudo

. 2011. Encapsulated follicular thyroid tumor with equivocal nuclear changes, so-called well-differentiated tumor of uncertain malignant potential: a morphological, immunohistochemical, and molecular appraisal. Cancer Sci, 102:288–294.

21.

Baloch

, LiVolsi

. 2000. Encapsulated follicular variant of papillary thyroid carcinoma with bone metastases. Mod Pathol, 13:861–865.

22.

Rivera

, Tuttle

, Patel

, Shaha

, Shah

, Ghossein

. 2009. Encapsulated papillary thyroid carcinoma: a clinico-pathologic study of 106 cases with emphasis on its morphologic subtypes (histologic growth pattern). Thyroid, 19:119–127.

23.

Mazzaferri

, Jhiang

. 1994. Long-term impact of initial surgical and medical therapy on papillary and follicular thyroid cancer. Am J Med, 97:418–428.

24.

Nwatsock

, Taieb

, Zok

, Mundler

. 2012. Late recurrences of thyroid carcinoma 24 years after a complete remission: when monitoring should be stopped?. World J Nucl Med, 11:42–43.

25.

Hauch

, Al-Qurayshi

, Randolph

, Kandil

. 2014. Total thyroidectomy is associated with increased risk of complications for low- and high-volume surgeons. Ann Surg Oncol, 21:3844–3852.

26.

Iyer

, Morris

, Tuttle

, Shaha

, Ganly

. 2011. Rising incidence of second cancers in patients with low-risk (T1N0) thyroid cancer who receive radioactive iodine therapy. Cancer, 117:4439–4446.