Model Protein BSA Adsorption and Covalent Coupling onto Methyl Methacrylate Based Latex Particles with Different Surface Properties

Abstract

The adsorption and covalent coupling of bovine serum albumin (BSA) onto methylmethacrylate (MMA) based monodisperse latex particles with different hydrophylic surfaces were investigated. P(MMA) and P(MMA/HEMA) microbeads in the size range of 1.5–2.0 mm were prepared by a dispersion homopolymerization method in the presence of poly(vinylpyrrolidone) (PVP) and poly(vinyl alcohol) (PVA) as steric stabilizer and by copolymerization of MMA and 2-hydroxethyl methacrylate (HEMA) where PVP was used as steric stabilizers. Surface properties of these particles were characterized in terms of contact angle measurements and FTIR-DRS spectra. Additionally, hydroxyl groups of PMMA_PVA and P(MMA/HEMA) particles were activated to provide aldehyde groups on surfaces. Adsorption of the BSA onto these five types of latex particles was examined as a function of initial albumin concentration, pH and ionic strength of the various cations (Na+,Ca+ and Mg− and anions (Cl⁻ and SCN⁻). The maximum adsorption capacities for all latex particles were obtained in solutions with 0.01μ m ionic strength and 1 mg/mL BSA concentration. When using PMMA_PVA, P(MMA/HEMA) and PMMA_PVP, the adsorption increased as hydrophobicity increased. Aldehyde activation of the two most hydrophobic particles elevated the coupling (immobilization) significantly in ionic strength of 0.01μ m. The adsorption and/or immobilization were maximum in the presence of monovalent cation, Na+ or anion, Cl−B for all types of latex particles.

Keywords

PMMA P(MMA/HEMA)BSA adsorption and coupling different polymeric surfaces

Get full access to this article

View all access options for this article.

References

1. Reunbaum, A. (ed.) (1991). Particles: Medical and Biological Applications, CRC Press, New York.

2. Cooney, D.O. (1984). In: Bekey, G.A. and Reneau, D.D. (eds .), Biomedical Engineering Principles: An Introduction to Fluid, Heat and Mass Transport Processes, Dekker, New York, Basel.

3. Davis, S.S. , Illium, L. , Mc Vie, J.G. and Tomlinson, E. (eds.) (1984). Particles and Drug Therapy, Pharmaceutical, Immunological and Medical Aspects, Elseviers Science, Amsterdam.

4. Rechnitz, G.A. (1987). J. Clin. Lab. Annu., 1: 308-308.

5. Gribnau, T.C.J. , Leuvering, J.H.W. and van Hell, H.J. (1986). Chromatogr., 376: 175-175.

6. Andrade, J.D. (ed.) (1985). In: Protein Adsorption, Surface and Interfacial Aspects of Biomedical Polymers, Vol. 2, Plenum, New York.

7. Elgersma, A.V. , Zsom, R.J. , Norde, W. and Lyklema, L.J. (1990). J. Colloid Interface Sci., 138(1): 145-145.

8. Tuncel, A. and Piskin, E. (1991). Biomat. Artif. Cells and Immobilized Reactants, 19: 229-229.

9. Tuncel, A. and Piskin, E. (1990). Polym. Plast. Technol. Eng., 29: 561-561.

10.

10. Barrett, K.E.J. (ed.) (1975). Dispersion Polym. in Organic Media, John Wiley, London.

11.

11. Almog, Y. and Levy, M. (1980). J. of Polymer Science, 18:1-1.

12.

12. Corner, T. (1981). Colloid Surf., 3: 119-119.

13.

13. Almog, Y. , Reich, S. and Levy, M. (1982). British Polym. J., 14: 131-131.

14.

14. Ober, C.K. , Lok, K.P. and Hair, M.L. (1985). J. Polym. Sci. Polym. Lett. Ed., 23: 103-103.

15.

15. Lok, K.P. and Ober, C.K. (1985). Can. J. Chem., 63: 209-209.

16.

16. Ober, C.K. and Lok, K.P. (1987). Macromol., 20: 268-268.

17.

17. Ober, C.K. and Hair, M.L. (1987). J. of Polym. Sci., 25: 1395-1395.

18.

18. Okubo, M. , Ykegami, K. and Yamamoto, Y. (1989). Colloid Polym. Sci., 267: 193-193.

19.

19. Bulmus, V. , Tuncel, A. and Piskin, E. (1996). J. of Applied Polymer Sci., 60: 697-697.

20.

20. Piskin, E. , Ayhan, H. , Rad, A.Y. , Bulmus, E.V. , Falkenhagen, D. and Weber,C. (1996). The International J. of Artificial Organs, 19(4): 124–127.

21.

21. Shen, S. , Sudol, E.D. and El-Aasser, M.S. (1994). J. of Polymer Science: Part A: Polymer Chemistry, 32: 1087–1100.

22.

22. Hou, K.C. , Zaniewski, R. and Roy, S. (1991). Biotech. Appl. Biochem., 13: 257-257.

23.

23. Ayhan, H. and Piskin, E. (1999). J. of Biomaterial Sci. Polymer Ed., 10: 109-109.

24.

24. Ayhan, H. , Bulmus, V. and Piskin, E. (1999). J. of Bioactive and Compatible Polymers, 14: 490-490.

25.

25. Ayhan, F. , Ayhan, H. , Pişkin, E. and Tanyolaç, A. (2002). Bioresource Technology, 81: 131-131.

26.

26. Suzawa, T. , Shirahama, H. and Fujimoto, T. (1982). J. Colloid Interface Sci., 86: 144-144.

27.

27. Suzawa, T. , Shirahama, H. and Fujimoto, T. (1983). J. Colloid Interface Sci., 93: 498-498.

28.

28. Shirahama, H. and Suzawa, T. (1985). J. Colloid Interface Sci., 104: 416-416.

29.

29. Ikeda, Y. , Iwata, H. , Horii, F. , Matsunaga, T. , Tanuguchi, M. , Suzuki, M. , Taki, W. , Yamagata, S. , Yonekawa, Y. and Handa, J. (1981). J. Biomed. Material Res., 15: 697-697.

30.

30. Carr, C.W. (1952). Arc. Biochem. Biophys., 40: 286-286.

31.

31. Scatchard, G. , Coleman, J.S. and Shen, A.L. (1957). J. Am. Chem. Soc., 79: 12-12.