The expression of cytokeratins in the epithelium of the middle ear and external auditory meatus of the rat was studied on cryosections of ethylenediaminetetraacetic acid–decalcified specimens by use of a panel of monoclonal antibodies. The normal middle ear epithelium revealed a complex cytokeratin profile, including regional differences. The induction of sterile middle ear effusions resulted in increased cytokeratin expression. Infective effusions were accompanied by both quantitative and qualitative changes in the cytokeratin expression patterns. The difference observed between the cytokeratin profiles of external meatal skin and those of middle ear epithelium may form a useful tool for research into cholesteatoma development.
LimDJShimadaT. Secretory activity of normal middle ear epithelium. Scanning and transmission electron microscopic observations. Ann Otol Rhinol Laryngol1971; 80: 319–29.
4.
KuijpersWJvan der BeekJMH. The effect of eustachian tube obstruction on middle ear mucosal transformation. In: BernsteinJMOgraPL, eds. Immunology of the ear. New York, NY: Raven Press, 1986: 205–29.
5.
BernsteinJM. Middle ear mucosa in health and disease. Arch Otolaryngol1971; 94: 30–5.
TosM. Experimental tubal obstruction. Changes in middle ear mucosa elucidated by quantitative histology. Acta Otolaryngol (Stockh)1981; 92: 51–61.
10.
MollRFrankeWWSchillerDJGeigerBKreplerR. The catalogue of human cytokeratins; patterns of expression in normal epithelia, tumors and cultured cells. Cell1982; 31: 11–24.
11.
SunTTTsengSCGHuaryAJW. Monoclonal antibody studies of mammalian epithelial keratin. A review. Ann NY Acad Sci1985; 455: 307–29.
12.
RamaekersFHuysmanAMoeskerO. Monoclonal antibody to keratin filaments specific for glandular epithelia and their tumors. Use in surgical pathology. Lab Invest1983; 49: 353–61.
13.
SadéJBabyatzkiAPinkusG. The metaplastic and congenital origin of cholesteatoma. Acta Otolaryngol (Stockh)1983; 96: 119–29.
14.
PalvaTLethoVPVirtanenIMakinenJ. Junctions of squamous epithelium with middle ear epithelium. Acta Otolaryngol (Stockh)1985; 99: 297–304.
15.
Van BlitterswijkCAGroteJJ. Cytokeration expression in cholesteatoma matrix, meatal epidermis and middle ear epithelium. Acta Otolaryngol (Stockh)1988; 105: 529–33.
16.
KuijpersWvan der BeekJMHWillaertECT. The effect of experimental tubal obstruction on the middle ear. Acta Otolaryngol (Stockh)1979; 87: 345–52.
17.
JonnsonRTarkowskiAKlareskosN. A demineralization procedure for immunohistopathological use. J Immunol Methods1986; 88: 109–14.
18.
RamaekersFHuysmanASchaartGMoeskerOVooysP. Tissue distribution of keratin 7 as monitored by a monoclonal antibody. Exp Cell Res1987; 170: 235–49.
19.
BroersJLVCarneyDNKleinbotM. Intermediate filament proteins in classic and variant type small cell lung carcinoma cell lines. J Cell Sci1986; 83: 37–60.
20.
LaneEBBartekJPurkisPELeighIM. Keratin antigens in differentiating skin. Ann NY Acad Sci1985; 455: 241–57.
21.
Van MuijenGNPRuiterDJFrankeWW. Cell type heterogeneity of cytokeratin expression in complex epithelia and carcinomas as demonstrated by monoclonal antibodies specific for cytokeratins nos. 4 and 13. Exp Cell Res1986; 162: 97–113.
22.
Van der BeekJMHKuijpersW. The mucoperiosteum of the middle ear in experimentally induced sterile otitis media. Acta Otolaryngol [Suppl] (Stockh)1983(suppl 414): 71–9.
23.
FeitzWFJDeBruyneFMJVooijsGPHermanCJRamaekersFCS. Intermediate filament proteins as tissue specific markers in normal and malignant urological tissues. J Urol1986; 136: 922–31.
24.
VerhageAPMAaldersTWRamaekersFCSDeBruyneFMJSchalkenJA. Differential expression of keratins in the basal and luminal compartments of rat prostatic epithelium during generation and regeneration. Prostate1988; 13: 25–38.
25.
NagleRBMollRWeidauerHNemetschekHFrankeWW. Different patterns of cytokeratin expression in the normal epithelia of the upper respiratory tract. Differentiation1985; 30: 130–40.
26.
QuinlanRASchillerDLHatzfeldM. Patterns of expression and organization of cytokeratin intermediate filaments. Ann NY Acad Sci1985; 455: 282–306.
27.
FrankeWWSchmidEWellsteedJGrundCGigiOGeigerB. Cytokeratin organization during the cell cycle: Selective masking of an immunologic determinant in PtK2 cells. J Cell Biol1983; 97: 1255–60.
