In order to elucidate the mechanisms of cisplatin (cis-diamminedichloroplatinum; CDDP)-resistant tumor cells, we previously established a CDDP-resistant KB cell line (KBrc cells) from a parental KB cell line derived from epidermoid carcinoma (KB cells). The KBrc cells were resistant to 5 kinds of platinum (Pt) drugs. Intracellular Pt concentrations in KBrc cells were lower than in KB cells. Decrease of intracellular Pt concentrations was one of the CDDP-resistant mechanisms. When we measured changes of intracellular calcium ion concentration ([Ca2+]i) during exposure to high-dose CDDP, a sustained elevation of the [Ca2+]i level was observed in the KB cells. These results suggest that the mechanisms underlying CDDP resistance involve changes in calcium channels and an alteration of calcium homeostasis in the tumor cell line.
LochrerPJ. Einhorn LH. Cisplatin. Ann Intern Med1984; 100: 704–13.
2.
ZwellingLAAndersonTKohnKW. DNA-protein and DNA interstrand cross-linking by cis- and trans-platinum (II) diamminedichloride in L 1210 mouse leukemia cells and relation to cytotoxicity. Cancer Res1979: 39: 365–9.
3.
OzolsRF. Pharmacologic reversal of drug resistance in ovarian cancer. Semin Oncol1985; 12: 7–11.
4.
TeicherBAHoldenSAKellyMJCharacterization of a human squamous carcinoma cell line resistant to cis-diammine dichloro platinum (II). Cancer Res1987; 47: 388–93.
5.
MannSCAndrewsPAHowellSB. Short-term cis-diammine dichloro platinum (II) accumulation on sensitive and resistant human ovarian carcinoma cells. Cancer Chemother Pharmacol1990; 25: 236–40.
6.
MeijerCMulderNHHospersGAPUrgesDRAVriesEGE. The role of glutathione in resistance to cisplatin in a human small cell lung cancer cell line. Br J Cancer1990; 62: 72–7.
7.
MasudaHTanakaTMatsudaHKusabaI. Increased removal of DNA-bound platinum in a human ovarian cancer cell line resistant to cis-diamminedichloro platinum (II). Cancer Res1990; 50: 1863–6.
8.
NishioKSugimotoYKasaharaKIncreased phosphorylation of nuclear phosphoproteins in human lung cancer cells resistant to cis-diammine dichloroplatinum (II). Int J Cancer1992; 50: 438–42.
BurkeyBBOmannGMWolfGT. Intracellular calcium changes associated with in vitro lymphokine-activated killer and natural killer cell cytotoxicity. Arch Otolaryngol Head Neck Surg1991; 117: 1281–6.
11.
BellomaGPerottiMTaddeiFTumor necrosis factor α induces apoptosis in mammary adenocarcinoma cells by an increase in intranuclear free Ca2+ concentration and DNA fragmentation. Cancer Res1992; 52: 1342–6.
12.
KleinJBSchepersTMDeanWLSonnenfeldGMcLeishKR. Role of intracellular calcium concentration and protein kinase C activation of IFN-γ stimulation of U937 cells. J Immunol1990; 144: 4305–11.
13.
EagleH.Propagation in a fluid medium of a human epidermoid carcinoma, strain KB. Proc Soc Exp Biol Med1955; 89: 362–4.
14.
HoriYKawamotoKYamazakiNKumazawaHYamashitaTKumazawaT. Establishment of a cisplatin-resistant KB cell line and its characterization. Acta OtolaryngolSuppl (Stockh) 1993(suppl 500): 142–8.
15.
FukuokaMNiitaniHHasegawaKPhase I study of new platinum compound, NK121 [Abstract]. Am Soc Clin Oncol1989; 8: 240.
16.
EndoKAkamatsuKMatsumotoTAntitumor activity of a new platinum complex, 2-aminomethylpyrrolidine (1, l-cyclobutane dicarboxylato) platinum (II). Anticancer Res1989; 9: 987–92.
17.
ShiratoriOKasaiHUchidaN. Antitumor activity of 254-S, a platinum complex, in rodents. In: Recent advances in chemotherapy. Anticancer section 1. Proceedings of the 14th International Congress of Chemotherapy. Tokyo, Japan: University of Tokyo Press, 1985: 635–6.
18.
TachikawaTKumazawaHHoriYChemosensitivity testing of human mouth carcinoma cell line. Acta OtolaryngolSuppl (Stockh) 1993(suppl 500): 154–7.
19.
LeRoyAFWehlingMLSponsellerHLFraiaufWSSolomonREDedrickRL. Analysis of platinum in biological materials by flameless atomic absorption spectrophotometry. Biochem Med1977; 18: 184–91.
20.
HanDYHaradaNTomodaKYamashitaT. Characterization of the calcium influx induced by depolarization of guinea pig cochlear spiral ganglion cells. ORL1994; 56: 125–9.
HaradaNHanDYKomedaMYamashitaT. Glutamate-induced intracellular Ca2+ elevation in isolated spiral ganglion cells of the guinea pig cochlea. Acta Otolaryngol (Stockh)1994; 114: 609–12.
23.
AndrewsPAVelurySMannSCHowellSB. Cis-diamminedichloro platinum (II) accumulation in sensitive and resistant human ovarian carcinoma cells. Cancer Res1988; 48: 68–73.
24.
VassilevPMKanazirskaMPCharamellaLJDimitrovNVTienHT. Changes in calcium channel activity in membranes from cis-diammine dichloroplatinum (II)-resistant and -sensitive L 1210 cells. Cancer Res1987; 47: 519–22.
25.
LazenbyCMThompsonMGHickmanJA. Elevation of leukemia cell intracellular calcium by the ether lipid SRI 62–834. Cancer Res1990; 50: 3327–30.
26.
BerglerWBierH. Cisplatin reduced epidermal growth factor receptors in squamous-cell carcinoma in vitro. ORL1990; 52: 297–302.
27.
HananskeAROsborneCKChamnessGL. Alteration of EGF receptor binding in human breast cancer cells by antineoplastic agents. Eur J Cancer Clin Oncol1987; 23: 545–51.
28.
BerridgeMJ. Inositol triphosphate and diacylglycerol as second messengers. Biochem J1984; 220: 345–60.
29.
NishizukaY. The role of protein kinase C in cell signal transduction and tumor promotion. Nature1984; 308: 693–8.
30.
BerglerWPetroianuGSchadelA. Improved antiproliferative effect of cisplatin combined with phorbol ester. An in vitro study. ORL J Otorhinolaryngol Relat Spec1993; 55: 100–4.
