Mineralocorticoid hormones regulate secretion and absorption in a wide variety of epithelial tissues, although specific mechanisms in the olfactory mucosa are currently unknown. Utilizing reverse transcription—polymerase chain reaction (RT-PCR) analysis, we have demonstrated the expression of mineralocorticoid (type I) receptor messenger RNA in the rodent olfactory mucosa. Amplification products of predicted size were obtained with nucleotide sequences corresponding to respective mineralocorticoid receptor (MR) kidney transcripts. Immunocytochemistry, using an antibody with known specificity for MRs, was then utilized in order to localize the cellular site(s) of MR protein expression in the olfactory mucosa. The highest levels of MR immunoreactivity were localized to the supranuclear region of sustentacular cells, as well as the acinar cells of the Bowman's glands. The respiratory regions of the nasal cavity were devoid of appreciable MR immunoreactivity. This study demonstrates both MR transcript and protein expression in the olfactory mucosa. We hypothesize that the mineralocorticoid hormones may have a role in modulation of olfactory secretion and/or sensory transduction in the peripheral olfactory system.
GetchellMMellertT. Olfactory mucus secretion. In: GetchellTDotyRBartoshukLSnowJ, eds. Smell and taste in health and disease. New York, NY: Raven Press, 1991: 83–95.
2.
GetchellMZielinskiBGetchellT. Odorant and autonomic regulation of secretion in the olfactory mucosa. In: MargolisFGetchellT, eds. Molecular neurobiology of the olfactory system. New York, NY: Plenum, 1988: 71–98.
3.
FarbmanA. Cell biology of olfaction. New York, NY: Cambridge Press, 1992: 24–72.
4.
ZielinskiBGetchellMWenokurRGetchellT. Ultrastructural localization and identification of adrenergic and cholinergic nerve terminals in the olfactory mucosa. Anat Rec1989; 225: 232–45.
5.
FanestilDDParkCS. Steroid hormones and the kidney. Annu Rev Physiol1981; 43: 637–49.
6.
FunderJW. Aldosterone action. Annu Rev Physiol1993; 55: 115–30.
7.
KernRCFosterJDPitovskiDZ. Mineralocorticoid (type I) receptors in the olfactory mucosa of the mammal: studies with aldosterone and the anti-mineralocorticoid spironolactone. Chem Senses1997; 22: 141–8.
8.
FongKKernRCPitovskiDZ. Regulation of Na,K-ATP-ase in the mammalian olfactory mucosa by aldosterone [Abstract]. Chem Senses1997; 22: 681.
9.
KernRCPitovskiDZ. Localization of 11β-hydroxysteroid dehydrogenase: specific protector of the mineralocorticoid receptor in mammalian olfactory mucosa. Acta Otolaryngol (Stockh)1997; 117: 738–43.
10.
DePaoloLMasoroE. Endocrine hormones in laboratory animals. In: LoebWQuimbyF, eds. The clinical chemistry of laboratory animals. New York, NY: Pergamon Press, 1989: 279–308.
11.
PatelPDShermanTGGoldmanDJWatsonSJ. Molecular cloning of a mineralocorticoid (type I) receptor complementary DNA from rat hippocampus. Mol Endocrinol1989; 3: 1877–85.
12.
RogersKEDasguptaPGublerUGrilloMKhew-GoodallYSMargolisFL. Molecular cloning and sequencing of a cDNA for olfactory marker protein. Proc Natl Acad Sci U S A1987; 84: 1704–8.
13.
ReydonJLSmithCA. The ultrastructure of normal and otosclerotic human stapes (preliminary studies). Laryngoscope1968; 78: 95–121.
14.
ArrizaJLWeinbergerCCerelliG. Cloning of human mineralocorticoid receptor complementary DNA: structural and functional kinship with the glucocorticoid receptor. Science1987; 237: 268–75.
15.
KrozowskiZSRundleSEWallaceC. Immunolocalization of renal mineralocorticoid receptors with an antiserum against a peptide deduced from the complementary deoxyribonucleic acid sequence. Endocrinology1989; 125: 192–8.
16.
FukushimaKSasanoHNaguroHSasakiIMatsunoSKrozowskiZS. Immunohistochemical localization of mineralocorticoid receptor in human gut. Tohoku J Exp Med1991; 165: 155–63.
17.
KatzA. Renal ATPase: its role in tubular sodium and potassium transport. Am J Physiol1982; 242: 207–19.
18.
MarverDMKokkoJP. Renal target sites and the mechanism of action of aldosterone. Miner Electrolyte Metab1983; 9: 1–18.
19.
BundgaardMMollerMPoulsenHH. Localization of sodium pump sites in cat salivary glands. J Physiol1977; 273: 339–53.
20.
CossuMLantiniMSPuxedduPRivaA. Cytochemical localization of ouabain-sensitive, K+-dependent p-nitrophenyl-phosphatase and Ca++-stimulated adenosine triphosphatase activities in human parotid and submandibular glands. Histochemistry1984; 81: 221–5.
21.
QuintonPMTormeyJM. Localization of Na+, K+-ATPase sites in the secretory and reabsorptive epithelia of perfused equine sweat glands: a question to the role of the enzyme in secretion. J Membr Biol1976; 29: 383–99.
22.
VangesaPHopferU. Localization of alkaline phosphatase and Na+ pump sites in adult rat and rabbit colon by cytochemical and cell fractionation techniques [Abstract]. J Cell Biol1978; 79: 238.
23.
CrabbeJ. Stimulation of active sodium transport by the isolated toad bladder with aldosterone in vitro [Abstract]. J Clin Invest1961; 42: 103.
24.
MillsJWErnstSA. Localization of sodium pump sites in frog urinary bladder. Biochim Biophys Acta1975; 375: 268–73.
25.
MillsJWErnstSADibonaDR. Localization of Na+-pump sites in frog skin. J Cell Biol1977; 73: 88–110.
26.
ErnstSMillsJ. Autoradiographic localization of tritiated ouabain—sensitive sodium pump sites in ion-transporting epithelia. J Histochem Cytochem1980; 28: 72–7.
27.
KernRCKerrTPGetchellTV. Ultrastructural localization of Na+/K+-ATPase in rodent olfactory epithelium. Brain Res1991; 546: 8–17.
28.
FosterJDKernRCPitovskiDZ. Localization of Na, K,ATPase in the mammalian olfactory mucosa [Abstract]. Association for Research in Otolaryngology Abstracts of the Eighteenth Midwinter Research Meeting. 1995: 650.
29.
PersaudKDeSimoneJGetchellMHeckGGetchellT. Ion transport across the frog olfactory mucosa: the basal and odorant-stimulated states. Biochim Biophys Acta1987; 902: 65–79.
30.
ReuterDZieroldKSchroderWFringsS. A depolarizing chloride current contributes to chemoelectrical transduction in olfactory sensory neurons in situ. J Neurosci1998; 18: 6623–30.
31.
McEwenBSJonesKPfaffD. Hormonal control of sexual behaviour in the female rat: molecular, cellular and neurochemical studies. Biol Reprod1987; 36: 37–45.
32.
MoguilewskyMRaynaudJ. Evidence for a specific mineralocorticoid receptor in rat pituitary and brain. J Steroid Biochem1980; 12: 309–14.
33.
MylesKFunderJW. Type I (mineralocorticoid) receptors in the guinea pig. Am J Physiol1994; 267: 268–72.
34.
NauntofteB. Regulation of electrolyte and fluid secretion in salivary acinar cells. Am J Physiol1992; 263: 823–37.
35.
RobinsonAMKernRCFosterJDFongKJPitovskiDZ. Expression of glucocorticoid receptor mRNA and protein in the olfactory mucosa: physiologic and pathophysiologic changes. Laryngoscope1998; 108: 1238–42.
36.
KernRCFosterJDPitovskiDZ. Glucocorticoid (type II) receptors in the olfactory mucosa of the guinea pig: RU 28362. Chem Senses1997; 22: 313–9.
