A bichromatic method is described for the determination of both total and direct-reacting bilirubin using a miniature centrifugal analyser. Bilirubin was coupled with diazotised sulphanilic acid in strongly acidic reaction conditions, and sodium dodecyl sulphate was used as accelerator for the measurement of total bilirubin. The inclusion of dyphylline as a haem-binding agent greatly reduced interference, even in the presence of extreme haemolysis. The method was shown to be precise, to be more sensitive and less affected by haemolysis than alternative methods, and to allow the estimation of total and direct bilirubin within the same run.
References
1.
JendrassikLGrofP. Vereinfachte photometrische Methoden zur Bestimmung des Blutbilirubins. Biochem Zschr1938; 34: 81–9.
2.
MichaelssonMNosslinBSjolinS. Plasma bilirubin determination in the newborn infant. Pediat1965; 35: 925–31.
3.
BillingBHaslamRWaldN. Bilirubin standards and the determination of bilirubin by manual and Technicon Auto Analyser methods. Ann Clin Biochem1971; 8: 21–30.
4.
GambinoSR. Bilirubin (Modified Jendrassik and Grof). In Standard methods of clinical chemistry, 5, MeitesS., Ed. New York: Academic Press1965; 55–64.
5.
CrossREHeintgesMGSavoryJAdaption of blank-corrected methods for measurements of total and conjugated bilirubin and uric acid to the centrifugal analyser. Clin Chem1976; 22: 429–33.
6.
KellyAMcKennaJPMcLellandAA bichromatic method for total bilirubin with a Centrifichem 400. Clin Chem1979; 25: 1482–4.
7.
BrodyJPValdesRSavoryJ. Centrifugal analyser method for total bilirubin in serum by use of diazotised 2-chloro-aniline-5-sulfonic acid. Clin Chem1979; 25: 205–8.
8.
WatanabeFTsubotaNMukaiHDetermination of total and direct bilirubin in serum with sodium dodecyl sulphate as an accelerator. Clin Chim Acta1978; 88: 149–53.
9.
NovrosJSKochTRKnoblockEC. Improved method for accurate quantitation of total and conjugated bilirubin in serum. Clin Chem1979; 25: 1891–9.
10.
Percy-RobbIWBroughtonPMGJenningsRDA recommended scheme for the evaluation of kits in the clinical chemistry laboratory. Ann Clin Biochem1980; 17: 217–26.
11.
HomsherRWesenbergJCBaginskiEWInteraction of haemoglobin in elevated serum total bilirubin determinations. Microchem J1979; 24: 526–35.
12.
ShullBCLeesHLiPK. Mechanism of interference by haemoglobin in the determination of total bilirubin. I. Method of Malloy-Evelyn. Clin Chem1980; 26: 22–5.
13.
ShullBCLeesHLiPK. Mechanism of interference by haemoglobin in the determination of total bilirubin. II. Method of Jendrassik-Grof. Clin Chem1980; 26: 26–9.
14.
BlanckaertNKabraPMFarinaFMMeasurement of bilirubin and its monoconjugates and diconjugates in human serum by alkaline methanolysis and high performance liquid chromatography. J Lab Clin Med1980; 96: 198–212.
