Sublethal plasma membrane disruption (PMD) is an established mechanism for signaling in several cell types, including endothelial cells and skeletal muscle. We used a rat model of orthodontic tooth movement to test the hypothesis that periodontal ligament (PDL) cells communicate stretch to changes in bone cell activity in part via PMD. To produce PMD, we used a 50-g load from a spring activated in the buccal direction against the maxillary first molars for 5 min. Uptake of endogenous serum albumin was used as a PMD marker. Immunohistochemistry demonstrates albumin in PDL cells surrounding moved first molar tips. Image analysis shows significantly more albumin in cells of the buccal side (tension) of the moved teeth compared with those of the lingual, distal, and mesial sides, and those of the unmoved control. Albumin localization within cells of the PDL, after only 5 min of mechanical loading, suggests that PMD could promote uptake or release of signaling molecules.
AbrahamJ.A., MergiaA., WhangJ.L., TumoloA., FriedmanJ., HjenildK.A.. (1986). Nucleotide sequence of a bovine clone encoding the angiogenic protein, basic fibroblast growth factor.Science233: 545–548.
2.
AuronP.E., WebbA.C., RosenwasserL.J., MucciS.F., RichA., WolffS.M.. (1984). Nucleotide sequence of human monocyte interleukin-1 precursor cDNA.Proc Natl Acad Sci USA81: 8463–8467.
3.
BorkeJ.L., MinamiJ., VermaA.K., PennistonJ.T., KumarR. (1987). Monoclonal antibodies to human erythrocyte membrane Ca++-Mg++ adenosine triphosphatase pump recognize an epitope in the basolateral membrane of human kidney distal tubule cell.J Clin Invest80: 1225–1231.
4.
BridgesT., KingG., MohammedA. (1998). The effect of age on tooth movement and meneral density in the alveolar tissue of the rat.Am J Orthod67: 67–72.
5.
BurgerE.H., Klein-NulendJ. (1999). Mechanotransduction in bone: role of the lacuno-canalicular network.FASEB J13(Suppl): S101–S112.
6.
ClarkM.S.F., KhakeeR., McNeilP.L. (1993). Loss of cytoplasmic basic fibroblast growth factor from physiologically wounded myofibers of normal and dystrophic muscle.J Cell Sci106: 121–133.
7.
ClarkM.S.F., CadwellR.W., ChiaoH., MiyakeK., McNeilP.L. (1995). Contraction-induced cell wounding and release of fibroblast growth factor in heart.Circ Res76: 927–934.
8.
DucyP., SchinkeT., KarsentyG. (2000). The osteoblast: a sophisticated fibroblast under central surveillance.Science289(5484): 1501–1504.
9.
FischerT.A., McNeilP.L., KhakeeR., FinnP., KellyR.A., PfefferM.A.. (1997). Cardiac myocyte membrane wounding in the abruptly pressure-overloaded rat heart under high wall stress.Hypertension30: 1041–1046.
10.
GibsonJ.M., KingG.J., KeelingS.D. (1992). Long-term orthodontic tooth movement response to short-term force in the rat.Angle Orthod62: 211–215, discussion 216.
11.
HillP.A. (1998). Bone remodelling.Br J Orthod25: 101–107.
12.
HsuS.M., RaineL., FangerH. (1981). Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures.J Histochem Cytochem29: 577–580.
13.
KlagsbrunM., VlodavskyI. (1988). Biosynthesis and storage of basic fibroblast growth factor (bFGF) by endothelial cells: implications for the mechanism of action of angiogenesis. In: Growth factors and other aspects of wound healing: biological and clinical implications.BarbulA., PinesE., CadwellM., HuntT.K., editors. New York: Alan R. Liss Inc., pp. 55–61.
14.
KyomenS., TanneK. (1997). Influences of aging changes in proliferative rate of PDL cells during experimental tooth movement in rats.Angle Orthod67: 67–72.
15.
LaemmliU.K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4.Nature Lond227: 680–685.
16.
McNeilP.L., ItoS. (1989). Gastrointestinal cells plasma membrane wounding and resealing in vivo.Gastroenterology96: 1238–1248.
17.
McNeilP.L., ItoS. (1990). Molecular traffic through plasma membrane disruption of cells in vivo.J Cell Sci96: 549–556.
18.
McNeilP.L., KhakeeR. (1992). Disruption of muscle fiber plasma membrane: role in exercise-induced damage.Am J Pathol40: 1097–1109.
19.
McNeilP.L., MuthukrishnanL., WarderE., D'AmoreP.A. (1989). Growth factors are released by mechanically wounded endothelial cells.J Cell Biol109: 811–822.
20.
ShiraziM., DehpourA.R., JafariF. (1999). The effect of thyroid hormone on orthodontic tooth movement in rats.J Clin Pediatr Dent23: 259–264.
21.
TeitelbaumS.L. (2000). Bone resorption by osteoclasts, Science289(5484): 1504–1508
22.
WalterP., LingappaV. (1986). Mechanism of protein translocation across the endoplasmic reticulum.Annu Rev Cell Biol2: 499–516.
23.
YoungP., HazudaD.J., SimonP.L. (1988). Human interleukin 1-β is not secreted from hamster fibroblast when expressed constitutively from a transfected cDNA.J Cell Biol107: 447–456.
