Epigallocatechin gallate (EGCg), the main antimicrobial tea catechin, has been reported to inhibit growth and virulence factors of oral pathogens in vitro. Although the mechanism is unclear, the potential of EGCg in reducing halitosis caused by volatile sulfur compounds (VSCs) has been suggested. This study tested the hypothesis that EGCg reduces VSCs by suppressing mgl, the gene encoding L-methionine-α-deamino-γ-mercaptomethane-lyase, responsible for methyl mercaptan (CH3SH) production by oral anaerobes. In this study, the effect of EGCg on in vitro growth, CH3SH production, and mgl gene expression in P. gingivalis W83 was investigated. EGCg inhibited growth of P. gingivalis W83 (MIC = 97.5 µg/mL) and was bactericidal (MBC = 187.5 µg/mL). At sub-MIC levels, EGCg inhibited CH3SH production, and mgl mRNA and protein expression (p < 0.05). We conclude that EGCg may represent a natural and alternative agent to the antimicrobial chemicals currently available for halitosis control.
CabreraCArtachoRGimenezR (2006). Beneficial effects of green tea—a review. J Am Coll Nutr25:79-99.
2.
CaturlaNVera-SamperEVillalainJMateoCRMicolV (2003). The relationship between the antioxidant and the antibacterial properties of galloylated catechins and the structure of phospholipid model membranes. Free Radic Biol Med34:648-662.
3.
ColiJMTonzetichJ (1992). Characterization of volatile sulphur compounds production at individual gingival crevicular sites in humans. J Clin Dent3:97-103.
Hamilton-MillerJM (2001). Anti-cariogenic properties of tea (Camellia sinensis). J Med Microbiol50:299-302.
6.
HoriHTakabayashiKOrvisLCarsonDANoboriT (1996). Gene cloning and characterization of Pseudomonas putida L-methionine-alpha-deamino-gamma-mercaptomethane-lyase. Cancer Res56:2116-2122.
7.
JohnsonPWYaegakiKTonzetichJ (1992). Effect of volatile thiol compounds on protein metabolism by human gingival fibroblasts. J Periodontal Res27:553-561.
8.
KhalichiPCvitkovitchDGSanterreJP (2004). Effect of composite resin biodegradation products on oral streptococcal growth. Biomaterials25:5467-5472.
KrespiYPShrimeMGKackerA (2006). The relationship between oral malodor and volatile sulfur compound-producing bacteria. Otolaryngol Head Neck Surg135:671-676.
11.
LeeKMKimWSLimJNamSYounMNamSW. (2009). Antipathogenic properties of green tea polyphenol epigallocatechin gallate at concentrations below the MIC against enterohemorrhagic Escherichia coli 0157:H7. J Food Prot72:325-331.
12.
LeeMJLambertJDPrabhuSMengXLuHMaliakalP. (2004). Delivery of tea polyphenols to the oral cavity by green tea leaves and black tea extract. Cancer Epidemiol Biomarkers Prev13:132-137.
13.
LodhiaPYaegakiKKhakbaznejadAImaiTSatoTTanakaT. (2008). Effect of green tea on volatile sulfur compounds in mouth air. J Nutr Sci Vitaminol (Tokyo)54:89-94.
14.
LoescheWJKazorC (2002). Microbiology and treatment of halitosis. Periodontol 200028:256-279.
15.
McKieAEEdlindTWalkerJMottramJCCoombsGH (1998). The primitive protozoon Trichomonas vaginalis contains two methionine gamma-lyase genes that encode members of the gamma-family of pyridoxal 5′-phosphate-dependent enzymes. J Biol Chem273:5549-5556.
16.
NakanoYYoshimuraMKogaT (2002a). Correlation between oral malodor and periodontal bacteria. Microbes Infect4:679-683.
17.
NakanoYYoshimuraMKogaT (2002b). Methyl mercaptan production by periodontal bacteria. Int Dent J52(Suppl 3):217-220.
18.
NgWTonzetichJ (1984). Effect of hydrogen sulfide and methyl mercaptan on the permeability of oral mucosa. J Dent Res63:994-997.
19.
NuderaWJFayadMIJohnsonBRZhuMWenckusCSBegoleEA. (2007). Antimicrobial effect of triclosan and triclosan with Gantrez on five common endodontic pathogens. J Endod33:1239-1242.
20.
OkamotoMSugimotoALeungKPNakayamaKKamaguchiAMaedaN (2004). Inhibitory effect of green tea catechins on cysteine proteinases in Porphyromonas gingivalis. Oral Microbiol Immunol19:118-120.
21.
PianottiRLachetteSDillsS (1986). Desulfuration of cysteine and methionine by Fusobacterium nucleatum. J Dent Res65:913-917.
22.
ReingewirtzYGiraultOReingewirtzNSengerBTenenbaumH (1999). Mechanical effects and volatile sulfur compound-reducing effects of chewing gums: comparison between test and base gums and a control group. Quintessence Int30:319-323.
23.
SakanakaSOkadaY (2004). Inhibitory effects of green tea polyphenols on the production of a virulence factor of the periodontal-disease-causing anaerobic bacterium Porphyromonas gingivalis. J Agric Food Chem52:1688-1692.
TunneyMMRamageGFieldTRMoriartyTFStoreyDG (2004). Rapid colorimetric assay for antimicrobial susceptibility testing of Pseudomonas aeruginosa. Antimicrob Agents Chemother48:1879-1881.
27.
van den BroekAMFeenstraLde BaatC (2008). A review of the current literature on management of halitosis. Oral Dis14:30-39.
28.
WuCDWeiGX (2002). Tea as a functional food for oral health. Nutrition18:443-444.
29.
WuCDWeiG (2009). Tea as a functional food for oral health. In: Food constituents and oral health: current status and future prospects. WilsonM, editor. Cambridge: Woodheading Publishing/CRC Press, pp. 396-417.
30.
YaegakiKSanadaK (1992). Volatile sulfur compounds in mouth air from clinically healthy subjects and patients with periodontal disease. J Periodontal Res27(4 Pt 1):233-238.
31.
YangCSLeeMJChenL (1999). Human salivary tea catechin levels and catechin esterase activities: implication in human cancer prevention studies. Cancer Epidemiol Biomarkers Prev8:83-89.
32.
YasudaHArakawaT (1995). Deodorizing mechanism of (-)-epigallocatechin gallate against methyl mercaptan. Biosci Biotechnol Biochem59:1232-1236.
33.
YoshimuraMNakanoYYamashitaYOhoTSaitoTKogaT (2000). Formation of methyl mercaptan from L-methionine by Porphyromonas gingivalis. Infect Immun68:6912-6916.
34.
YoshimuraMNakanoYFukamachiHKogaT (2002). 3-Chloro-DL-alanine resistance by L-methionine-alpha-deamino-gamma-mercaptomethane-lyase activity. FEBS Lett523:119-122.
35.
YoungAJonskiGRöllaGWalerSM (2001). Effects of metal salts on the oral production of volatile sulfur-containing compounds (VSC). J Clin Periodontol28:776-781.
