The elaboration of dentin-pulp engineering strategies requires the investigation of not only progenitor cell potentials but also their interactions with other non-progenitor “supportive” cells. Under severe caries lesions, progenitor cells may be activated by growth factors released after the acidic dissolution of carious dentin. However, dentin regeneration has also been observed after traumatic injuries without any significant dentin dissolution. This raises questions about the origin of signals involved in progenitor cell activation, migration, and differentiation. Study models such as the entire tooth culture and co-cultures of pulp and endothelial cells highlighted the role of interactions between the different pulp cell types and the pivotal role they play in dentin regeneration. Injured pulp fibroblasts secrete growth factors involved in progenitor cell activation and differentiation as well as neoangiogenesis which may pave the pathways for progenitor cell migration. This appears to be the first paper to focus on this very important field in dental pulp biology.
AboutIBotteroMJDe DenatoPCampsJFranquinJCMitsiadisTA (2000a). Human dentin production in vitro. Exp Cell Res258:33-41.
2.
AboutILaurent-MaquinDLendahlUMitsiadisTA (2000b). Nestin expression in embryonic and adult human teeth under normal and pathological conditions. Am J Pathol157:287-295.
3.
AboutICampsJMitsiadisTAButlerWFranquinJC (2002a). Influence of resinous monomers on the differentiation in vitro of human pulp cells into odontoblasts. J Biomed Mater Res Appl Biomater63:418-423.
4.
AboutIProustJ-PRaffoSMitsiadisTAFranquinJ-C (2002b). In vivo and in vitro expression of connexin 43 in human teeth. Connect Tissue Res43:232-237.
5.
ArteseLRubiniCFerreroGFioroniMSantinelliAPiattelliA (2002). Vascular endothelial growth factor (VEGF). Expression in healthy and inflamed human dental pulps. J Endod28:20-23.
6.
BatouliSMiuraMBrahimJTsutsuiTWFisherLWGronthosS. (2003). Comparison of stem-cell-mediated osteogenesis and dentinogenesis. J Dent Res82:976-981.
7.
BrautAKollarEJMinaM (2003). Analysis of the odontogenic and osteogenic potentials of dental pulp in vivo using a Col1a1-2.3-GFP transgene. Int J Dev Biol47:281-292.
8.
CassidyNFaheyMPrimeSSSmithAJ (1997). Comparative analysis of transforming growth factor-beta isoforms 1–3 in human and rabbit dentine matrices. Arch Oral Biol42:219-223.
9.
FinkelmanRDMohanSJenningsJCTaylorAKJepsenSBaylinkDJ (1990). Quantitation of growth factors IGF-I, SGF/IGF II and TGF-ß in human dentin. J Bone Min Res5:717-723.
10.
FitzgeraldMChiegoDJHeysDR (1990). Autoradiographic analysis of odontoblast replacement following pulp exposure in primate teeth. Arch Oral Biol35:707-715.
11.
GoldbergMSmithAJ (2004). Cells and extracellular matrices of dentin and pulp: a biological basis for repair and tissue engineering. Crit Rev Oral Biol Med15:13-27.
12.
GronthosSMankaniMBrahimJRobeyPGShiS (2000). Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo. Proc Natl Acad Sci USA97:13625-13630.
13.
GronthosSBrahimJLiWFisherLWChermanNBoydeA. (2002). Stem cell properties of human dental pulp stem cells. J Dent Res81:531-535.
14.
GuKSmokeRHRutherfordRB (1996). Expression of genes for bone morphogenetic proteins and receptors in human dental pulp. Arch Oral Biol41:919-923.
15.
InoueTShimonoM (1992). Repair dentinogenesis following transplantation into normal and germ-free animals. Proc Finn Dent Soc88(Suppl 1):183-194.
16.
IoharaKZhengLWakeHItoMNabekuraJWakitaH. (2008). A novel stem cell source for vasculogenesis in ischemia: subfraction of side population cells from dental pulp. Stem Cells26:2408-2418.
17.
LillesaarCErikssonCJohanssonCSFriedKHildebrandC (1999). Tooth pulp tissue promotes neurite outgrowth from rat trigeminal ganglia in vitro. J Neurocytol28:663-670.
18.
MartinP (1997). Wound healing: aiming for perfect skin regeneration. Science276:75-81.
19.
MathieuSEl-BattariADejouJAboutI (2005). Role of injured endothelial cells in the recruitment of human pulp stem cells. Arch Oral Biol50:109-113.
20.
MatsushitaKMotaniRSakutaTYamaguchiNKogaTMatsuoK. (2000). The role of vascular endothelial growth factor in human dental pulp cells: induction of chemotaxis, proliferation, and differentiation and activation of the AP-1-dependent signaling pathway. J Dent Res79:1596-1603.
21.
MouleAJLiHBartoldPM (1995). Donor variability in the proliferation of human dental pulp fibroblasts. Aust Dent J40:110-114.
22.
NosratIVSmithCAMullallyPOlsonLNosratCA (2004). Dental pulp cells provide neurotrophic support for dopaminergic neurons and differentiate into neurons in vitro; implications for tissue engineering and repair in the nervous system. Eur J Neurosci19:2388-2398.
23.
Roberts-ClarkDSmithAJ (2000). Angiogenic growth factors in human dentine matrix. Arch Oral Biol45:1013-1016.
24.
SakaiVTZhangZDongZNeivaKGMachadoMAShiS. (2010). SHED differentiate into functional odontoblasts and endothelium. J Dent Res89:791-796.
25.
ShiSGronthosS (2003). Perivascular niche of postnatal mesenchymal stem cells in human bone marrow and dental pulp. J Bone Miner Res18:696-704.
26.
ShibaHNakamuraSShirakawaMNakanishiKOkamotoHSatakedaH. (1995). Effects of basic fibroblast growth factor on proliferation, the expression of osteonectin (SPARC) and alkaline phosphatase, and calcification in cultures of human pulp cells. Dev Biol170:457-466.
27.
SloanAJSmithAJ (1999). Stimulation of the dentine-pulp complex of rat incisor teeth by transforming growth factor-beta isoforms 1–3 in vitro. Arch Oral Biol44:149-156.
28.
SloanAJRutherfordRBSmithAJ (2000). Stimulation of the rat dentine-pulp complex by BMP7 in vitro. Arch Oral Biol45:173-177.
29.
SmithAJMurrayPESloanAJMatthewsJB (2001). Matrix influences on dental cytodifferentiation and dentin regeneration. In: Proceedings, Dentin-Pulp Complex Meeting, 2001. Chiba, Japan: Quintessence Publishing Co., Ltd., pp. 39-44.
30.
TéclèsOLaurentPZygouritsasSBurgerASCampsJDejouJ. (2005). Activation of human dental pulp stem cells in response to odontoblast injury. Arch Oral Biol50:103-108.
31.
TéclèsOLaurentPAubutVAboutI (2008). Human tooth culture: a study model for reparative dentinogenesis and direct pulp capping materials biocompatibility. J Biomed Mater Res B Appl Biomater85:180-187.
32.
Tran-HungLMathieuSAboutI (2006). Role of human pulp fibroblasts in angiogenesis. J Dent Res85:819-823.
33.
Tran-HungLLaurentPCampsJAboutI (2008). Quantification of angiogenic growth factors released by human dental cells after injury. Arch Oral Biol53:9-13.
34.
TsujiTTakeiKInoueTShimonoMYamamuraT (1987). An experimental study on wound healing of surgical exposed dental pulps in germ-free rats. Bull Tokyo Dent Coll28:35-38.
35.
TziafasDSmithAJLesotH (2000). Designing new treatment strategies in vital pulp therapy. J Dent28:77-92.
