The Application of a Micro-Elution Technique Using Anti-Human Globulin for the Detection of the S,S,K,Fy a,Fy b and JK a Antigens in Stains

Abstract

A micro-elution technique using anti-globulin is described which increases the number of blood group antigens detectable in bloodstains. When compared with the older inhibition technique it is shown to be not only markedly more sensitive but to require far less stained material for each individual test.

Yet another advantage is that the antigens detectable by this method are those which appear to withstand well the passage of time by remaining active in stains for many months. This is in contrast to the majority of red cell enzymes which, while being readily detectable, do not usually give readable results beyond about 8 weeks.

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