Comparison of a Rapid Dipstick Test and Thick Blood Films for Detecting Parasites of Plasmodium Falciparum Used under Typical Conditions at a Semi-Rural Hospital in Cote D'Ivoire

Abstract

This prospective study compares a rapid dipstick test (ParaSight™-F) with thick blood films for the detection of parasites of P falciparum, under ‘typical’ conditions and constraints to be found in a semi-rural hospital in a tropical developing country in Africa. Eighty-two samples were tested using the two techniques and found to concur in 95.1% of cases. However, in four of the samples the results differed. The thick blood films of 60 samples were later re-read by a local reference laboratory. Of these 98.3% were in agreement with the reading performed at the hospital. Only one of the 60 slides differed.

The rapid dipstick test proved to be both easy to use and free from many of the usual constraints such as a need for formally trained or experienced laboratory staff, laboratory equipment, and reliable water and electricity supplies.

In an holoendemic area for P falciparum transmission, it would appear to be eminently suitable, in technical terms and ease of handling as well as on the basis of rapid results, for wider distribution within this region. Its main drawback remains financial.

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References

Niangue

Bassalia

. Programme National de Lutte Contre le Paludisme. République de Côte d'Ivoire: Ministère de la Santé et de la Protection Sociales, 1992

Henry

Van Leeuwen

Watson

. In vivo sensitivity of Plasmodium falciparum to chloroquine in rural areas of Côte d'Ivoire. Acta Tropica 1994; 58: 275–81

Genton

Smith

Baea

Narara

Al-Yaman

Bech

H-P

. Malaria: how useful are clinical criteria for improving the diagnosis in a highly endemic area? Trans R Soc Trop Med Hyg 1994; 88: 537–41

Bottius

Guanzirolli

Trape

J-F

Rogier

Konate

Druilhe

. Malaria: even more chronic in nature than previously thought; evidence for subpatent parasitaemia detectable by the polymerase chain reaction. Trans R Soc Trop Med Hyg 1996; 90: 10

Hakim

Furuta

Noor Rain

. Diagnosis of malaria using a plate hybridization method for the detection of polymerase chain reaction products. Trans R Soc Trop Med Hyg 1995; 89: 271–2

Baird

Purnomo Jones

. Diagnosis of malaria in the field by fluoresence microscopy of QBC™ capillary tubes. Trans R Soc Trop Med Hyg 1992; 86: 3–5

Delacollette

Van Der Stuyft

. Direct acridine orange staining is not a ‘miracle’ solution to the problem of malaria diagnosis in the field. Trans R Soc Trop Med Hyg 1994; 88: 187–8

Lowe

Jeffa

New

Pedersen

Engback

Marsh

. Acridine orange fluorescence techniques as alternatives to traditional Giemsa staining for the diagnosis of malaria in developing countries. Trans R Soc Trop Med Hyg 1996; 90: 34–6

Knobloch

Henk

. Screening for malaria by determination of parasite-specific lactate dehydrogenase. Trans R Soc Trop Med Hyg 1995; 89: 269–70

10.

Parra

Evans

Taylor

. Identification of Plasmodium falciparum hisitidine-rich protein 2 in the plasma of humans with malaria. J Clin Microbiol 1991; 29: 1629–34

11.

Suvari

Kennedy

Msaki

Ramsay

Bidwell

Voller

. Clinical evaluation of an antigen detection ELISA for Plasmodium falciparum malaria. Trans R Soc Trop Med Hyg 1993; 87: 375.

12.

Shiff

Premji

Minjas

. The rapid manual ParaSight™-F test. A new diagnostic tool for Plasmodium falciparum infection. Trans R Soc Trop Med Hyg 1993; 87: 1646–8

13.

Beadle

Long

Weiss

McElroy

Maret

Oloo

. Diagnosis of malaria by detection of Plasmodium falciparum HRP-2 antigen with a rapid dipstick antigen-capture assay. Lancet 1994; 343: 564–8

14.

Becton

Dickinson

. A Review of the Performance Evaluations of the ParaSight™ F Rapid Test for P falciparum malaria. Becton Dickinson

15.

Premji

Minjas

Shiff

. Laboratory diagnosis of malaria by village health workers using the rapid manual ParaSight™-F test. Trans R Soc Trop Med Hyg 1994; 88: 418