A Method for Measuring the Concentration of Co ₂ Released By Entomopathogenic Nematodes

Abstract

Spanish

This study aimed to standardize a method for measuring the concentration of CO_₂ released by infective juveniles (IJ's), in order to assess their metabolic activity. The nematodes used in this experiment were Heterorhabditis amazonensis JPM4 and Steinernema carpocapsae All. A standardized gas chromatography (GC) method was used for CO₂ analysis. There was a linear increase in CO₂ concentration associated with increased numbers of IJs. Additionally, the CO₂ concentration obtained for S. carpocapsae was higher than that obtained for H. amazonensis. The standardized GC method was adequate to measure the concentration of CO₂ released by IJ's.

Keywords

Metabolism Standardization Steinernematidae Heterorhabditidae

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