The availability of a collection of fetal and embryonic spines made possible a review of the involution of the notochord. Results of this histological, histochemical and immunohistochemical study are in accord with the dominant view in the literature that the notochord does not contribute materially to the adult nucleus pulposus. It is also consonant with the expectation that, normally, all chordal cells have disappeared during early childhood, but is not sufficiently detailed to assess the possibility of occasional survivors.
GegenbaurC.Elements of comparative anatomy.London: Macmillan and Co,1878
2.
HertwigO.Lehrbuch der Entwicklungsgescichte des Menschen und Wierbeltiere.Jena: Gustav Fischer,1890
3.
WilliamsL.W.The later development of the notochord in mammals.Am J Anat1908; 8: 251–84
4.
KeyesD.C., CompereE.L.The normal and pathological physiology of the nucleus pulposus of the intervertebral disk.J Bone Joint Surg1932; 14: 879–935
5.
VirchowR.Untersuchungen uber die Entwicklungen des Schadelgrundes im gesunden und wrankhaften Zustande und uber den Einfluss derselben auf Schadelform.Berlin: Gesichtsbildung und Gehirnban, Reimer,1857
6.
HeibergJ.Uber die Zwischenwerbeigelenke und Knockenkerne der Werbeisaule bei den Neugeboren und ihre Verhaltniss zur Chorda.Mitt Embryol Inst Univ Wien1880; 1: 119–29
7.
WeissA.Die Entwicklung der Wirbensanle der weissen Ratte, besonders der vardensten Halswirbel.Z Wiss Zool1901; 69: 492–533
8.
WeissA.(1952) Observations on the post-natal structure of the intervertebral disc in man.J Anat1952; 86: 162–79
9.
RuggeriA.Ultrastructural, histochemical and auto-radiographic studies on the developing chick notochord.Z Anat EntwGesch1972; 138: 20–33
10.
SalisburyJr., WattF.M.Lack of keratan sulphate in the human notochord.J Anat1988; 157: 175–9
11.
PeacockA.Observations on the pre-natal development of the intervertebral disc in man.J Anat1951; 85: 260–74
12.
SalisburyJr.Personal communication,1987
13.
WalmsleyR.The development and growth of the intervertebral disc.Edinburgh Med J1953; 60: 341–63
14.
StreeterG.L.Weight, sitting height, head size, foot length, and menstrual age of the human embryo.Contrib Embryol1921; 11: 143–70
15.
SalisburyJr., IsaacsonP.G.Demonstration of cytokeratins and epithelial membrane antigen in chordomas and human foetal notochord.Am J Surg Pathol1985; 9: 791–7
16.
MakinC.H., BobrowL.G., BodmerW.F.Monoclonal antibody to cytokeratin for use in routine histo-pathology.J Clin Pathol1984; 37: 975–83.
17.
BurchellJ., DurbinH., Taylor-PapdimitriouJ.Complexity of expression of antigenuc determinants recognised by monoclonal antibodies HMFG-1 and HMFG-2 in normal and malignant human mammary epithelial cells.J Immunol1983; 131: 508–13
18.
HsuS-M, RaineL., FangerH.The use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques.J Histochem Cytochem1981; 29: 577–80
19.
PostonR.N., SidhuY.S., MakinC., MasonD.V.Specific enzyme treatment is required for individual monoclonal antibodies in immunohistochemistry with formalin fixed sections.Proc Protides of the Biological Fluids1984; 32: 557–60
LoeweL.Zur Kentnisse der Saengetierchorda.Arch mikr Anat1879; 16: 597–612
22.
AmprinoR., BairatiA.Studi sulle trasformazioni delle cartaligini dell'uomo nell'accrescimento e nella sene-scenza. Parte III.Z Zellforsch1934; 21: 440–82
23.
PraderA.Die Frühembryonale Entwicklung der mens-lichen Zwischenwirbelscheibe.Acta Anat1947; 3: 68–83
24.
SchaefferJ.Die Ruckenseite der Saugtiere nach der Geburt nebst Bemerkungen uber den Ban und die Vermocherung der Wirbel.S B Akad Wiss Wien (Abt III)1910; 119: 409–65
25.
FickR.Handbuch der Anatomie und Mechanik der Gelenke.Part I. Jena: Gustav Fischer,1904
26.
KoellikerA.Entwicklungsgeschicte des Mencken und hoeren Tiere.Leipzig: Wilhelm Engelmann,1879
27.
TaylorJr.Persistence of the notochordal canal in vertebrae.J Anat1972; 111: 211–17