Preliminary in vitro studies have been performed to assess the effect of experimental lipid emulsions of varying fatty acid composition on human adipose tissue metabolism. Subcutaneous human adipose tissue was obtained during elective surgery and placed in tissue culture. Physical mixtures of long chain triglyceride (LCT) and/or medium chain triglyceride (MCT) were added to the tissue culture medium so that the final concentration was 400 mg/dl. After a 3-day incubation period the tissue was harvested, placed in buffer and used to determine in vitro production of the prostaglandins prostacyclin I2 (measured as its stable endproduct 6-keto PGF1α), thromboxane A2 (measured as TXB2), and prostaglandin E2. Measurements of the fatty acid profile found in the neutral- and phospholipid fraction of the adipose tissue and fat cell size were also made. The results demonstrate that samples incubated in 100% MCT had the most significant increase in prostaglandin production whereas those incubated in 100% LCT had the most significant decrease in activity of the three prostaglandins assayed, when compared to controls. The addition of LCT to MCT caused a dose-related decrease in adipose tissue prostaglandin production. There were no significant changes in the profile of fatty acids found in the neutral- or phospholipid fraction of adipose tissue. The results indicate that the relative level of MCT/LCT incubated with human adipose tissue has a significant effect on prostaglandin production. (Journal of Parenteral and Enteral Nutrition12:178-184, 1988)
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References
1.
Package insert 20% Travamulsion, Travenol Laboratories, Deer-field, IL
2.
Package insert 20% Liposyn, Abbott Laboratories, North Chicago, IL
3.
Collins FD, Sinclair AJ, Royle JP, et al: Plasma lipids in human linoleic acid deficiency. Nutr Metab13:150-167, 1971
4.
Paulsrud JR, Pensler L., Whitten CF, et al: Essential fatty acid deficiency in infants induced by fat-free intravenous feeding. Am J Clin Nutr25:897-904, 1972
5.
Caldwell MD, Jonsson HT, Otherson HB: Essential fatty acid deficiency in an infant receiving prolonged parenteral alimentation. J Pediatr81:894-898, 1972
6.
Connor WE: Pathogenesis and frequency of essential fatty acid deficiency during total parenteral nutrition. Ann Int Med93:895-896, 1975
7.
Wene JD, Connor WE, DenBesten L.: The development of essential fatty acid deficiency in healthy men fed fat-free diets intravenously and orally. J Clin Invest56:127-134, 1975
8.
Riella MC, Broviac JW, Wells M., et al: Essential fatty acid deficiency in human adults during total parenteral nutrition. Ann Int Med83:786-789, 1975
9.
Richardson TJ , Sgoutas D.: Essential fatty acid deficiency in four adult patients during total parenteral nutrition. Am J Clin Nutr28:258-263, 1975
10.
Stegink LD, Freeman JB, Wispe J., et al: Absence of the biochemical symptoms of essential fatty acid deficiency in surgical patients undergoing protein sparing therapy. Am J Clin Nutr30:388-393, 1977
11.
Katz DP, Timmers KI, Knittle JL: Differences in prostaglandin production from adipose tissue of obese and nonobese subjects. Diabetes30 (suppl 1):128A, 1981
12.
Katz DP, Knittle JL: Glucose tolerance and adipose tissue prostaglandin production. Int J Obesity7 (suppl 1):23A, 1983
13.
Axelrod L., Levine L.: Prostacyclin production by isolated adipocytes. Diabetes30:163-167, 1981
14.
Christ EJ, Nugteren DH: The biosynthesis and possible function of prostaglandins in adipose tissue . Biochim Biophys Acta218 :296-307, 1970
15.
Mathias MM, Dupont J.: The relationship of dietary fats to prostaglandin biosynthesis. Lipids14:247-252, 1979
16.
Rossner S.: Studies on an intravenous fat tolerance test. Methodological experimental and clinical experience with Intralipid. Acta Med Scand Suppl564:1-24, 1974
17.
Phillips HJ: Dye exclusion test for cell viability. IN Tissue Culture Methods and Applications, Kruse Jr PF , Patterson MK (eds). Academic Press, New York, 1973, pp 406-408
18.
Chen RF: Removal of fatty acids from serum albumin by charcoal treatment. J Biol Chem242:173-181, 1967
19.
Hirsch J., Gallian E.: Methods for the determination of adipose cell size in man and animals . J Lipid Res9:110-119, 1968
20.
Touchstone JC, Chen JC, Beaver KM: Improved separation of phospholipids in thin layer chromatography. Lipids15:61-62, 1979
21.
Skipski VP, Barclay M.: Thin-layer chromatography of lipids. IN Methods in Enzymology, Vol XIV, Lowenstein JM (ed). Academic Press, New York, 1969, pp 531-598
22.
Glass RL: Alcoholysis, saponification and preparation of fatty acid methyl esters . Lipids6:919-923, 1971
23.
Mahadevan V. , Zieve L.: Determination of volatile free fatty acids of human blood. J Lipid Res10:338-341, 1969
24.
Skrinska V. , Lucas F.: Isolation of prostacyclin from whole blood . Prostaglandins22:365-375, 1981
25.
Granstrom E. , Kindall H.: Radioimmunoassay of prostaglandins and thromboxanes . IN Advances in Prostaglandin and Thromboxane Research, Vol 5, Frolich JC (ed). Raven Press, New York, 1978, pp 119-210
26.
Axelrod L., Levine L.: Plasma prostaglandin levels in rats with diabetes mellitus and diabetic ketoacidosis . Diabetes31:994-1001, 1982
27.
Dray F., Gerozissis K., Kouznetzova B., et al: New approach to the RIA of prostaglandins and related compounds using iodinated tracers. IN Advances in Prostaglandin and Thromboxane Research, Vol 6, Samuelsson B, Ramwell PW, Paoletti R (eds). Raven Press, New York, 1980, pp 167-180
28.
Levine L.: Levels of 13,14-dihydro-15-keto-PGE2 in some biological fluids as measured by radioimmunoassay. Prostaglandins14:1125-1139, 1977
29.
Demers LM, Derck DD: A radioimmunoassay for 6-keto-prostaglandin F1α. IN Advances in Prostaglandin and Thromboxane Research, Vol 6, Samuelsson B, Ramwell PW , Paoletti R (eds). Raven Press, New York, 1980, pp 193-199
30.
Levine L., Gutierrez Cernosek RM, Van Vunakis H.: Specificities of prostaglandin B1, F1α, and F2α antigen-antibody reactions . J Biol Chem246:6782-6785, 1971
Van Dorp D. : Recent developments in the biosynthesis and analyses of prostaglandins. Ann NY Acad Sci180:181-199, 1971
33.
Tan WC, Privett OS: Studies of the synthesis of prostaglandins in the vesicular glands of essential fatty acid-deficient and hypophysectomized rats. Biochim Biophys Acta296:586-592, 1973
34.
Struijk CB, Beerthuis RK, Pabon JHH, et al: Specificity in the enzymic conversion of polyunsaturated fatty acids into prostaglandins. Rec Trav Chim85:1233-1239, 1966
35.
Ziboh VA, Vanderhoek JY, Lands WEM: Inhibition of sheep vesicular gland oxygenase by unsaturated fatty acids from skin of essential fatty acid deficient rats. Prostaglandins5:233-240, 1974
36.
Van Evert WC , Nugteren DH, Van Dorp DA: Inhibition of prostaglandin biosynthesis by c-5, c-8, c-11 eicosatrienoic acid. Prostaglandins15:267-272, 1978
37.
Vincet JE, Zijlstra FJ: Formation by phospholipase A2 of prostaglandins and thromboxane A2-like activity in the platelets of normal and essential fatty acid deficient rats. Comparison with effect on human and rabbit platelets. Prostaglandins14:1043-1053, 1977
38.
Hulan HW, Kramer JKG: The effect of long-chain monoenes on prostaglandin E2 synthesis by rat skin . Lipids12:604-609, 1977
39.
Meydani S., Mathias M., Schatte C.: Dietary fat type and ambient oxygen tension influence pulmonary prostaglandin synthetic potential. Prostaglandins Med1:241-249, 1978
40.
Schoene NW, Reeves VB, Ferretti A.: Effects of dietary linoleic acid on the synthesis of PGE2 and PGF2 in kidney medullae in spontaneously hypertensive rats. IN Advances in Prostaglandin and Thromboxane Research, Vol 8, Samuelsson B, Ramwell PW , Paoletti R (eds). Raven Press, New York, 1980, pp 1791-1792
41.
Nugteren DH, van Evert WC, Soeting WJ, et al: Effect of different amounts of linoleic acid in the diet on the excretion of urinary prostaglandin metabolites in the rat. IN Advances in Prostaglandin and Thromboxane Research, Vol 8, Samuelsson B, Ramwell PW , Paoletti R (eds). Raven Press, New York, 1980, pp 1793-1796
42.
Lipinski BA , Mathias MM: Prostaglandin production and lipolysis in isolated rat adipocytes as affected by dietary fat. Prostaglandins16:957-963, 1978
43.
Epstein M., Lifshitz M., Rappaport K.: Augmentation of prostaglandin production by linoleic acid in man. Clin Sci63:565-571, 1982
44.
Review of Physiological Chemistry, Chapt 22, Harper HA, Rodwell VW , Mayes PA (eds). Lange Medical Publication, Los Altos, CA, 1979, p 321
45.
Kaunitz H., Slanetz CA, Johnson RE, et al: Medium-chain and long-chain saturated triglycerides and linoleic acid requirements. J Nutr71:400-404, 1960
46.
Hirsch J., Farquar JW, Ahrens EH, et al: Studies of adipose tissue in man. A microtechnic for sampling and analysis. Am J Clin Nutr8:499-511, 1960
47.
Hashim SA, Asfour RH: Tocopherol in infants fed diets rich in polyunsaturated fatty acids . Am J Clin Nutr21:7-14, 1968
48.
Dayton S., Hashimoto S., Dixon W., et al: Composition of lipids in human serum and adipose tissue during prolonged feeding of a diet high in unsaturated fat. J Lipid Res7:103-111, 1966
49.
Dalton C., Hope WC: Cyclic AMP regulation of prostaglandin biosynthesis in fat cells. Prostaglandins6:227-242, 1974
50.
Jeremy JY, Mikhailidis DP, Dandona P.: Simulating the diabetic environment modifies in vitro prostacyclin synthesis . Diabetes32:217-221, 1983
51.
Pace-Asciak C., Wolfe LS: Inhibition of prostaglandin synthesis by oleic, linoleic, and linolenic acids. Biochim Biophys Acta152:784-787, 1968
52.
Ziboh VA: Biosynthesis of prostaglandin E2 in human skin: Subcellular localization and inhibition by unsaturated fatty acids and anti-inflammatory drugs. J Lipid Res14:377-384, 1973
53.
Denning GM, Figard PH, Spector AA: Effect of fatty acid modification of prostaglandin production by cultured 3T3 cells. J Lipid Res23:584-596, 1982
54.
Friedman Z., Seyberth H., Frolich J., et al: Effect of dietary variation in linoleic acid content on the major urinary metabolites of the E prostaglandins (PGE-M) in infants. IN Advances in Prostaglandin and Thromboxane Research, Vol 8, Samuelsson B, Ramwell PW , Paoletti R (eds). Raven Press, New York, 1980, pp 1799-1805
55.
Andersen DW , Filer LJ, Stegink LD: Intravenous lipid emulsions in the treatment of essential fatty acid deficiency: Studies in young pigs . Pediatr Res18:1350-1355, 1984
