Insulin-Like Growth Factor 1 Stimulates Protein Synthesis and Inhibits Protein Breakdown in Muscle From Burned Rats

Background: Bum injury is associated with substantial whole-body protein loss, reflecting mainly a catabolic response in skeletal muscle. Recent studies suggest that treatment with insulin-like growth factor 1 (IGF-1) may reverse the catabolic response to burn injury, but the effects of IGF-1 on muscle protein synthesis and breakdown rates after bum injury are not known. We tested the hypothesis that IGF-1 blunts the catabolic response in skeletal muscle after burn injury by stimulating protein synthesis and inhibiting protein breakdown and that this effect of IGF-1 is caused by a direct effect on muscle tissue. Methods: Intact extensor digitorum longus muscles from burned, sham-burned, and untreated rats were incubated in the absence or presence of different concentrations of IGF-1. Total and myofibrillar protein breakdown rates were measured as net release of tyrosine and 3-methylhistidine, respectively. Protein synthesis rates were determined by measuring the incorporation of (U-¹⁴C)-phenylalanine into protein. Results: IGF-1 stimulated protein synthesis and inhibited protein breakdown in a dose-dependent fashion in muscles from burned and unburned rats. The maximal effect of IGF-1 on protein synthesis was seen at a hormone concentration of 100 ng/mL, whereas protein breakdown was further inhibited when the hormone concentration was increased to 1 μg/mL. Ubiquitin messenger RNA (mRNA) levels were reduced by IGF-1 in incubated muscles, suggesting that IGF-1 may inhibit ubiquitin-dependent protein breakdown. Conclusions: These results suggest that the anabolic effects of IGF-1 after burn may reflect inhibited protein breakdown and stimulated protein synthesis in skeletal muscle and that this response may be caused by a direct effect of IGF-1 on muscle tissue. (journal of Parenteral and Enteral Nutrition 21:245-251, 1997)