Alanyl-Glutamine-Supplemented Parenteral Nutrition Increases Luminal Mucus Gel and Decreases Permeability in the Rat Small Intestine

Abstract

Background: Effect of supplemental alanyl-glutamine in standard TPN (S-TPN) on luminal mucus gel and small intestinal permeability was investigated. Methods: Thirty Sprague-Dawley rats were divided into group I (n = 10), receiving standard rat diet; group II (n = 10), receiving S-TPN; and group III (n = 10), receiving alanyl-glutamine-supplemented TPN for 1 week. After 1 week, fluorescein isothiocyanate (FITC)-dextran was injected into the small intestine of the rats, and they were killed. A small intestinal sample and portal blood were obtained for morphologic and functional analysis of mucus gel and intestinal permeability. Results: In group II, thickness and optical density of mucus gel per millimeter serosal length of intestine were significantly lower than group I (p < .001) and were significantly higher in group III than in group II (p < .001). The number of goblet cells in the villi and in the crypt of the small intestine was significantly lower in group II than in group I (p < .001) and was significantly higher in group III than in group II (p < .001), with the exception of the villi of jejunum. Villous and crypt surface area per millimeter serosal length of intestine was significantly lower in group II than in group I (p < .001) and was significantly higher in group III than in group II (p < .001). Small intestinal permeability to FITC-dextran was significantly higher in group II than in group I (p < .001) and was significantly lower in group III than in group II (p < .001). Glucosamine synthetase level was significantly higher in group III than in group I and ileum of group II (p < .001). Conclusions: Alanyl-glutamine-supplemented TPN prevents a decrease in mucus gel and an increase in small intestinal permeability associated with S-TPN. (Journal of Parenteral and Enteral Nutrition 23:24-31, 1999)

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References

Alverdy JC , Aoys E. , Moss GS : TPN promote bacterial translocation from the gut. Surgery 104:185-190, 1988

Purandare S. , Offenbart K. , Westrom BR , et al: Increased gut permeability to fluorescent isothiocyanate-dextran after TPN in the rat. Scand J Gastroenterol 24:678-682, 1989

Tamada H. , Nezu R. , Matsuo Y. , et al: Addition of alanyl-glutamine into TPN prevents intestinal mucosal atrophy in rats. JPEN 16:110-116, 1992

Iiboshi Y. , Nezu R. , Kennedy M. , et al: TPN decreases luminal mucous gel and increases permeability of small intestine. JPEN 18:316-320, 1994

Scheppah W. , Loges C. , Bartram P. , et al: Effect of free glutamine and alanyl-glutamine dipeptide on mucosal proliferation of the human ileum and colon. Gastroenterology 107:429-434, 1994

Cairnie AB : Renewal of goblet and paneth cells of small intestine. Cell Tissue Kinet 3:35-45, 1970

Forstner J. , Taichman N. , Kalvin V. , et al: Intestinal goblet cell mucus. J Cell Sci 12:588-602, 1973

Szentkuti L. , Eggers A. : Stabilisation of preepithelial mucus gel in cryostat sections from rat colon with celloidin. Stain Technol 65:179-181, 1990

Goodman MJ , Kent PW , Truelove SC : Glucosamine synthetase activity of the colonic mucosa in ulcerative colitis and Crohn's disease. Gut 18:219-228, 1977

10.

Deitch EA : BT of gut flora. Proceedings of NIH Conference on Advances in Understanding Trauma and Burn Injury. 30:5184-5188, 1990

11.

Leloir LF , Cardini CE : The biosynthesis of glucosamine. Biochim Biophys Acta 12:15-22, 1953

12.

Lukie BE , Forstner GG : Synthesis of intestinal glycoprotein. Incorporation of [I-14C]-glycosamine in vitro. Biochim Biophys Acta 261:353-364, 1972

13.

Windmullar HG , Spaeth AE : Respiratory fuels and nitrogen metabolism in vivo in small intestine of fed rats. J Biol Chem 255:107-113, 1980

14.

Wilmore DW , Smith RJ O'Dwyer ST , et al: The gut: A central organ after surgical stress. Surgery 104:917-923, 1988

15.

Vander Hulst RR , Van Kreel BK , Von Meyelfeldt MF , et al: Glutamine and preservation of gut integrity. Lancet 334:1363-1365, 1993

16.

Naka S. , Saito H. , Hashiguchi Y. , et al: Alanyl-glutamine supplemented TPN improves survival and protein metabolism in rat protracted bacterial peritonitis model. JPEN 20:417-423, 1996

17.

Bollard JE , Vanderwee MA , Smith GW , et al: Preservation of mucus in situ rat colon. Dig Dis Sci 31:1338-1344, 1986

18.

Sander DL , Dudrick SJ , Johnson RL : Influence of method of feeding on stress ulcer development in the rat. Dig Dis Sci 25:279-283, 1980

19.

Winterburn PJ Phelps CF : Studies on the control of hexosamine biosynthesis by glucosamine synthetase. Biochem J 121:711-720, 1971

20.

Okubo H. , Chandler MA : Endocrine status and the response of glucosamine-6-phosphate synthetase to trauma. Exp Mol Pathol 24:91-98, 1976

21.

Schonhofer P. , Anspach KF : Die Aktivitat der L-glutamin-D-fruc-6-phosphat-carrageenin induzierten entzungdung und ihre hemmbarkeit durch phenylbutazon. Arch Int Pharmacodyn 166: 382-388, 1967

22.

Li J. , Henken LB , Suzuki K. , et al: Glutamine prevents TPN induced increases in small intestinal permeability. JPEN 18:303-307, 1994