The effects of insulin-like growth factor-1 (IGF-1) on the catabolic and immune response induced by thermal injury were studied in burned rats fed by TPN. An increase of synthesis greater than the increase of breakdown resulted in improved nitrogen retention in the IGF-1 group. There was no effect on the mRNA in the structural proteins of the skeletal muscle and liver. However, the gene expression of albumin and the structural proteins of the diaphragm increased significantly in the rats receiving IGF-1. The proliferation of the gut mucosa and the fractional protein synthesis rate of the small intestine increased, and the endotoxin content of the liver and spleen were smaller in the burned rats that received IGF-1. Delayed type hypersensitivity increased significantly (p < .01) in the IGF-1 group. In conclusion, IGF-1 improved the whole-body protein metabolism, and albumin and respiratory muscle protein synthesis in the burned rats. It significantly promoted the proliferation of the intestinal mucosa, and reduced the intestinal translocation of endotoxin. Cellular immunity was also enhanced. (Journal of Parenteral and Enteral Nutrition23:S93-S97, 1999)
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References
1.
Tashiro T., Yamamori H., Takagi K., et al: Effect of protein dose on nitrogen retention in postoperative patients receiving TPN. Surg Res Commun18:231-238, 1996
2.
Sandstrom S. , Drott C., Hyltander A., et al: The effect of postoperative intravenous feeding (TPN) on outcome following major surgery evaluated in a randomized study. Ann Surg217:185-195, 1993
3.
Shimoda N., Tashiro T., Yamamori H., et al: Effects of growth hormone and insulin-like growth factor-1 on protein metabolism, gut morphology and cell-mediated immunity in burned rats . Nutrition13:540-546, 1997
4.
Tashiro T., Takagi K., Mashima Y., et al: Recombinant human growth hormone improves postoperative protein economy of patients receiving esophagectomy. IN Diseases of Esophagus, Springer-Verlag, Tokyo, 1993, pp 1017-1023
5.
Sugiura T., Tashiro T., Yamamori H., et al : Effect of IGF-1 on endotoxin translocation in burned rats receiving TPN. Nutrition13:783-787, 1997
6.
Picou D., Taylor-Roberts T.: The measurement of total protein synthesis and catabolism and nitrogen turnover in infants in different nutritional states and receiving different amount of dietary protein. Clin Sci36:283-296, 1969
7.
Stein TP, Oram-Smith JC, Leskin MJ, et al: Effect of nitrogen and calorie restriction on protein synthesis in the rats. Am J Physiol230:1321-1325, 1976
8.
Ino H., Ishizuka T., Chiba T., et al: Expression of CDK5 (PSSALRE kinase), a neural cdc2-related protein kinase, in the mature and developing mouse central and peripheral nervous systems. Brain Res661:196-201, 1994
9.
Tomas FM, Knowles SE, Owens PC, et al: Effects of full-length and truncated insulin-like growth factor-1 on nitrogen balance and muscle protein metabolism in nitrogen-restricted rats . J Endocrinol128:97-105, 1991
10.
Fang CH, Li BG, James JH, Fischer JE, et al: The anabolic effects of IGF-1 in skeletal muscle after burn injury are not caused by increased cell volume. JPEN22:115-119, 1998
11.
Jacob R., Barrett E., Plewe G., et al: Acute effects of Insulin-like growth factor-1 on amino acid metabolism in the awake fasted rat. J Clin Invest83:1717-1723, 1989
12.
Crown AL, Holly JMH: The insulin-like growth factor system in critical illness: pathophysiology and therapeutic potential. Clin Nutr14:321-328, 1995
13.
Lemmy AB, Martin AA, Read LC, et al: IGF-1 and structured analog des-(1-3)IGF-1 enhance growth in rats after gut resection. Am J Physiol260(Endocrinol Metab23):E213-219, 1991
14.
Ho KK, O'Sullivan AJ, Hoffman DM: Metabolic action of growth hormones in man. Endocr J43 (Suppl):S57-63, 1996
15.
Dickerson RN , Manzo CB, Charland RG, et al: The effect of insulin-like growth factor-1 on protein metabolism and hepatic response to endotoxemia in parenterally fed rats. J Surg Res58:260-266, 1995
16.
Sugiura T., Tashiro T., Yamamori H., et al: Effect of growth hormone on mRNA levels of structural protein in skeletal muscle and the diaphragm of burned rats. Jpn J Surg Met Nutr29:151-156, 1995
17.
Mealy K., Barry M., O'Mahony L. et al: Effects of human recombinant growth hormone (rhGH) on inflammatory responses in patients undergoing abdominal aortic aneurysm repair. Intensive Care Med24:128-131, 1998
18.
Nurse P.: Universal control mechanism regulating onset of M-phase. Nature344:503-508, 1990
19.
Surmacz E., Nugent P., Pietrzkowski Z., et al: The role of the IGF-1 receptor in the regulation of cdc-2 mRNA levels in fibroblasts. Exp Cell Res199:275-278, 1992
20.
Huang KF, Chung DH, Herndon DN: Insulin-like growth factor-1 (IGF-1) reduces gut atrophy and bacterial translocation after severe burn injury. Arch Surg128:47-54. 1993
21.
Alexander JW , Gianotti L., Pyles T., et al: Distribution and survival of Escherichia coli translocating from the intestine after thermal injury. Ann Surg213:558-566; 1991
22.
Spaeth G., Gottwald T., Specian RD, et al: Secretory immunoglobulin A, intestinal mucin, and mucosal permeability in nutritionally induced bacterial translocation in rats. Ann Surg220:798-808, 1994
23.
Deitch EA, Winterton J., Berg RD: Thermal injury promotes bacterial translocation from the gastrointestinal tract in mice with impaired T-cell mediated immunity. Arch Surg121:97-101, 1986
24.
Deitch EA, Maejima K., Berg RD: Effects of oral antibiotics and bacterial overgrowth on the translocation of the GI-tract microflora in burned rats. J Trauma25:385-392, 1985
25.
Marshall JC , Christou NV, Meakins JK: The gastrointestinal tract. The "undrained abscess" of multiple organ failure. Ann Surg213:111-119, 1993
26.
Takagi K., Suzuki F., Brrow RE, et al: Growth hormone improves the resistance of thermally injured mice infected with herpes simplex virus type 1. J Trauma44:517-522, 1998
27.
Balteskard L., Unneberg K., Halvorsen D., et al: Effects of insulin-like growth factor-1 on neutrophil and monocyte functions in normal and septic states. JPEN22:127-135, 1998
28.
Kudsk KA, Mowatt-Lassen C., Bukar J., et al: Effect of recombinant human insulin-like growth factor-1 and early TPN on immune depression following severe head injury. Arch Surg129:66-71, 1994
29.
Cioffi WG, Gore DC, Rue LW III , et al: Insulin-like growth factor-1 lowers protein oxidation in patients with thermal injury. Ann Surg220:310-319, 1994
30.
Goeters C., Mertes N., Tacke J., et al: Repeated administration of recombinant human insulin-like growth factor-1 in patients after gastric surgery. Ann Surg122:646-653, 1995
31.
Young SCJ , Clemmons DR: Changes in insulin-like growth factor(IGF)-binding proteins after IGF-linjections in insulin-dependent diabetics. J Clin Endocrinol Metab78:609-614, 1994
