Immunohistopathologic Analysis of Olfactory Degeneration Caused by Ischemia

Abstract

The development of olfactory dysfunction caused by ischemia was studied in Mongolian gerbils. Mongolian gerbils frequently have an anomaly of the cerebral circulation and are susceptible to brain ischemia or infarction following ligation of a single common carotid artery. Ischemia was induced by unilateral common carotid artery ligation or temporary occlusion of both common carotid arteries, and the olfactory pathway was examined. In the olfactory pathway of the forebrain, ischemic changes were observed in the lateral olfactory tract, olfactory tubercle, olfactory ventricle, and anterior olfactory nucleus. The olfactory bulb was resistant to ischemia. Partial or complete degeneration of the ipsilateral olfactory neuroepithelium was observed in some gerbils that survived more than 14 days after the onset of ischemia. Immunohistopathologic analysis of the neuroepithelium for the olfactory marker protein revealed that functional damage of the olfactory neurons occurred in some gerbils within the first few days after the ischemic event.

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References

Doty

: A review of olfactory dysfunctions in man. Am J Otolaryngol 1:57–79, 1979.

Shafa

Meisami

Mousavi

: Retarding effect of early anosmia on growth of the body, brain, olfactory bulbs, and cerebellum and its implication for the development of the olfactory system in rat. Exp Neurol 67:215–233, 1980.

Matulionis

Breipohl

Bhatnagar

: Degeneration and regeneration of olfactory epithelium in the mouse. Ann Otol Rhinol Laryngol 91(suppl 89):1–12, 1982.

Graziadei

GA Monti

Graziadei

PPC

: Neurogenesis and neuron regeneration in the olfactory system of mammals. II. Degeneration and reconstitution of the olfactory sensory neurons after axotomy. J Neurocytol 8:197–213, 1979.

Simmons

Rafols

Getchell

: Ultrastructural changes in olfactory receptor neurons following olfactory nerve section. J Comp Neurol 197:237–257, 1981.

Le vine

Payan

: Effect of ischemia and other procedures on the brain and retina of the gerbils (Meriones unguiculatus). Exp Neurol 16:255–262, 1966.

Nakashima

Kimmelman

Snow

: Histopathology of the olfactory pathway due to ischemia. Laryngoscope 94:171–175, 1984.

Kahn

: The natural course of experimental cerebral infarction in the gerbil. Neurology 22:510–516, 1972.

Margolis

: A marker protein for the olfactory chemoreceptor neuron. In Bradshaw

Schneider

, editors: Proteins of the nervous system, ed 2. New York, 1980, Raven Press, pp 59–84.

10.

Keller

Margolis

: Immunological studies of the rat olfactory marker protein. J Neurochem 24:1101–1106, 1975.

11.

Sternberger

Hardy

Jr Cuculis

Meyer

: The unlabeled antibody enzyme method of immunohistochemistry. Preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-antihorseradish peroxidase) and its use in identification of spirochetes. J Histochem Cytochem 18:315–333, 1970.

12.

Margolis

: A brain protein unique to the olfactory bulb. Proc Natl Acad Sci USA 69:1221–1224, 1972.

13.

Harding

Graziadei

PPC

Graziadei

GA Monti

Margolis

: Denervation in the primary olfactory pathway of mice. IV. Biochemical and morphological evidence for neuronal replacement following nerve section. Brain Res 132:11–28, 1977.

14.

Graziadei

GA Monti

: Experimental studies on the olfactory marker protein. III. The olfactory marker protein in the olfactory epithelium lacking connections with the forebrain. Brain Res 262:303–308, 1983.