A reproducibility study of the model cavity system for evaluating the in vitro cytotoxicity of dental restorative materials was undertaken at Birmingham Dental School and Huntingdon Research Centre. Using a precise experimental protocol drawn up following liaison between the two centres, a number of materials were tested independently. Toxicity was assessed by measurement of reduction in fibroblast and macrophage numbers relative to controls. Overall reproducibility was good, and the ranking of the toxicity of the test materials by both centres was almost identical.
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2.
MeryonS.D., and BrowneR.M. (1983). Test methods for assessing the cytotoxicity of dental restorative materials using an in vitro model cavity system. In Ceramics in Surgery (ed. VincenziniP.), pp. 127–135. Elsevier Scientific Publishing Company: Amsterdam.
3.
MeryonS.D., and BrowneR.M. (1983). Evaluation of the cytotoxicity of four dental materials in vitro assessed by cell viability and enzyme cytochemistry.Journal of Oral Rehabilitation10, 363–372.
4.
MeryonS.D., StephensP.G., and BrowneR.M. (1983). A comparison of the in vitro cytotoxicity of two glass ionomer cements.Journal of Dental Research6, 769–773.
5.
MeryonS.D., and BrowneR.M. (1984). In vitro cytotoxicity of a glass ionomer cement of a new generation.Cell Biochemistry and Function2, 43–48.
6.
MeryonS.D. (1984). The influence of dentine on the in vitro cytotoxicity testing of dental restorative materials.Journal of Biomedical Materials Research18, 771–779.
7.
MeryonS.D., and StephensP.G. (1983). Fluoride as the possible cytotoxic component of Silicap. 2. Effect on cellular enzymes in different cell types.International Endodontic Journal16, 26–32.
8.
MeryonS.D., and RichesD.W.H. (1982). A comparison of the in vitro cytotoxicity of four restorative materials assessed by changes in enzyme levels in two cell types.Journal of Biomedical Materials Research16, 519–528.
