Presence of Three Philadelphia Chromosomes (Ph 1 ) in the Blastic Crisis of a Case of Chronic Myeloid Leukemia

Abstract

Italian

Three Philadelphia chromosomes in addition to other extra-chromosomes in most of the bone marrow cells (70%) were observed in the terminal course of a case of chronic myeloid leukemia with blastic crisis. The percentage distribution of extra-chromosomes in the single groups of the Denver system was evaluated and compared with the theoretical expectation. This analysis showed a distribution of extra-chromosomes widely different from the random expectation, several chromosome groups being highly underrepresented (namely A, B, D and E) and others (C, F and G) overrepresented. From the karyotype analysis the commonest feature (27,4%) appeared to be the presence of three extra-chromosomes in group C, one extra in group F, and two extra Ph¹ in group G. The leukocyte alkaline phosphatase performed on smears of peripheral blood gave a total value higher than normal. Various workers have connected the low values usually observed in chronic myeloid leukemia with the loss in chromosome 21 genetic material. In the present case, the increase in leukocyte alkaline phosphatase alongside an increase in chromatin 21 material might suggest a correlation between these two parameters.

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