A Comparative Immunoelectrophoretic Study on Extracts of LS Fibroblasts and of Tumors Produced by Inoculating them into C3H Mice.

Aqueous extracts of LS fibroblasts (LS) and of tumors obtained by inoculating these cells into newborn C3H mice (Tu/LS) share a number of antigens detectable by means of Immunoelectrophoresis against rabbit anti-LS or anti-Tu/LS sera. Yet, at least one antigen seems to be contained only in the LS extracts; in fact, anti-LS sera, absorbed with Tu/LS extracts, reacted with the LS extracts, causing a precipitation line on Immunoelectrophoresis. This may depend on the suppression from the growing population of the inoculated cells of a line more incompatible or in any case less favored in the environment of the subcutaneous tissue of the living animal; or on the lack of in vivo production of an antigen that the LS fibroblast sinthesizes when growing in vitro.