The H1 fraction in Oberling Guerin-Guerin's myeloma was isolated, and an electrophoretic study was carried out in order to establish its composition. The results obtained show that the H1 fraction is heterogeneous and is formed of 2 sub-fractions. These were separated by preparative gel electrophoresis. They differ in molecular weight, electrophoretic mobility, and primary structure. Each sub-fraction exists in 2 different electric states.
Get full access to this article
View all access options for this article.
References
1.
BalhornR., RiekeW. O., ChalkleyR.: Rapid electrophoretic analysis for histone phosphorylation. A reinvestigation of phosphorylation of lysine-rich histone during rat liver regeneration. Biochemistry, 10: 3952–3959, 1971.
2.
BalhornR., BalhornM., MorrisH. P., ChalkleyR.: Comparative high-resolution electrophoresis of tumor histone: Variation in phosphorilation as a function of cell replication rate. Cancer Res., 32: 1775–1784, 1972.
3.
BustinM., ColeR. D.: Species and organ specificity in very lysine-rich histones. J. Biol. Chem., 243: 4500–4505, 1968.
4.
CroftL. R.: Handbook of protein sequences.Joynsons and Bruwers Ltd., Oxford, 1973.
5.
JohnsE. W.: Studies on histones. Biochem. J., 92: 55–59, 1964.
6.
KinkadeJ. M.: Qualitative species differences and quantitative tissue differences in the distribution of lysine-rich histones. J. Biol. Chem., 244: 3375–3386, 1969.
7.
LowryO. H., RosebroughN. J., FarrA. L., RandallR. J.: Protein measurement with the folin phenol reagent. J. Biol. Chem., 193: 265–275, 1951.
8.
MooreS., SteinW. H.: Chromatographic determination of amino acid by the use of automatic recording equipment. Vol. VI, pp. 819–831. In: Methods in Enzymology.ColowichS. P., KaplanN. O. (eds.), Academic Press, New York, 1963.
9.
PanyimS., ChalkleyR.: High resolution acrylamide gel electrophoresis of histones. Arch. Biochem. Biophys., 130: 337–346, 1969.
10.
PanyimS., ChalkleyR., SpikerS., OliverD.: Constant electrophoretic mobility of the cysteine-containing histone in plants and animals. Biochim Biophys. Acta, 214: 216–221, 1970.
11.
PanyimS., BilekD., ChalkleyR.: An electrophoretic comparison of vertebrate histones. J. Biol. Chem., 246: 4206–4215, 1971.
12.
PanyimS., ChalkleyR.: The molecular weights of vertebrate histones exploiting a modified sodium dodecyl sulfate. Electrophoretic method J. Biol. Chem., 246: 7557–7560, 1971.
13.
ReisfeldR. A., LewisU. J., WilliamsD. E.: Disk electrophoresis of basic proteins and peptides on polyacrylamide gel. Nature, 195: 281–283, 1962.
14.
SherodD., JohnsonG., ChalkleyR.: Studies on the heterogeneity of lysine-rich histones in dividing cells. J. Biol. Chem., 249: 3923–3931, 1974.
15.
StellwagenR. H., ColeR. D.: Comparison of histones obtained from mammary gland at different stages of development and lactation. J. Biol. Chem., 243: 4456–4462, 1968.
