The leucocyte inhibition factor (LIF) test, revealing the inhibition of leucocyte migration from capillary tubes, was studied with leucocytes from 47 patients with breast cancer, from 14 patients with benign breast tumors, and from 56 normal subjects. As antigens we used formalin-fixed tumor cells. The LIF assay was positive in 38/47 (80.8 %) breast cancer patients, in 10/14 (71.4 %) benign breast tumor patients, and in 13/56 (23.2 %) normal subjects. The negative LIF assays were higher with leucocytes from patients with metastases than with those from patients with no metastases. We found no correlation between the LIF-test response and the histopathologic pattern of the tumors, type of treatment (almost always surgical), and remission or relapse state of our patients at the time of testing.
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References
1.
AndersonV., BjerrumO., BendixenG., SchiodtT., DissingI.: Effects of autologous mammary tumour extracts on human leucocytes migration in vitro. Int. J. Cancer, 5: 357–363, 1970.
2.
BrandesL. J., GoldenbergG. J.: Peripheral leucocyte migration inhibition reactivity to breast cancer antigens in patients with breast cancer and in normal controls. Cancer Res., 36: 3707–3710, 1976.
3.
CochranA. J., GrantR. M., SpilgW. G., MackieR. M., RossC. E., HoyleD. E., RussellJ. M.: Sensitization to tumor-associated antigens in human breast carcinoma. Int. J. Cancer, 14: 19–25, 1974.
4.
CochranA. J., MackieR. M., RossC. E., OggL. J., JacksonA. M.: Leucocyte migration inhibition by cancer patients sera. Int. J. Cancer, 18: 274–281, 1976.
5.
CochranA. J., SpilgW. G. S., MackieR. M., ThomasC. E.: Postoperative depression of tumor-directed cell-mediated immunity in patients with malignant diseases. Br. Med. J., 4: 67–70, 1972.
6.
DrakeW. P., UngaroP. C., MardineyM. R.: Preservation of cellular antigenicity of tumor cells by the use of formalin fixation. Cancer Res., 32: 1042–1044, 1972.
7.
EliasG. E., EliasL. L.: Autogenous leucocyte migration in human malignancies. Cancer, 36: 1393–1398, 1975.
8.
GeorgeM., VaughanJ. H.: In vitro cell migration as a model for delayed hypersensitivity. Proc. Soc. Exp. Biol. Med., 111: 514–521, 1962.
9.
HellströmI., HellströmK. E., EvansC. A.: Serum-mediated protection of neoplastic cells from inhibition by lymphocytes immune to their tumor-specific antigens. Proc. Nat. Acad. Sci. USA, 60: 362–369, 1969.
10.
HellströmK. E., HellströmI.: Lymphocyte-mediated cytotoxicity and blocking serum activity to tumor antigens. Adv. Immunol., 18: 209–277, 1974.
11.
JonesB. M.: Mechanism of leucocyte migration inhibition by breast tumor cell fractions. Br. J. Cancer, 34: 14–19, 1976.
12.
JonesB. M., ConnollyC. E., IsaacsonP., TurnerD. T. L., TurnbullA. R.: Tumor-directed leucocyte migration inhibition in operable breast cancer: Additional clinical correlations. Br. J. Cancer, 34: 94–96, 1976.
13.
JonesB. M., TurnbullA. R.: Horizontal studies of cell-mediated immune-reactions to autologous tumor-antigens in patients with operable mammary carcinoma. Br. J. Cancer, 32: 339–344, 1975.
14.
KadishA. S., MarcusD. M., BloomB. R.: Inhibition of leukocyte migration by human breast-cancer-associated antigens. Int. J. Cancer, 18: 581–586, 1976.
15.
McCoyJ. L., JeromeL. F., DeanJ. M., CannonG. B., AlfordT. C., DoeringT., HerbermanR. B.: Inhibition of leukocyte migration by tumor-associated antigens in soluble extracts of human breast carcinoma. J. Nat. Cancer Inst., 53: 11–17, 1974.
16.
RossC. E., CochranA. J., HoyleD. E., GrantR. M., MackieR. M.: Formalin-fixed tumor cells in the leucocyte migration test. Lancet, 2, 1087–1088, 1973.
17.
RossC. E., CochranA. J., HoyleD. E., GrantR. M., MackieR. M.: Formalinized tumor cells in the leucocyte migration inhibition test. Clin. Exp. Immunol., 22: 126–132, 1975.
18.
SegallA., WeilerO., GeminJ., LacourJ., LacourF.: In vitro study of cellular immunity against autochthonous human cancer. Int. J. Cancer., 9: 417–425, 1972.
19.
SöborgM., BendixenG.: Human lymphocyte migration as a parameter of hypersensitivity. Acta Med. Scand., 181: 247–256, 1967.
20.
TognellaS., MantovaniG., FlorisC., CengiarottiL., Del GiaccoG. S., ManconiP. E., GrifoniV.: Tecniche per la evidenziazione del fattore di inibizione dei macrofagi (MIF) e del fattore di inibizione leucocitaria (LIF). pp. 189–193. In: Metodiche immunologiche.MelliG., MazzeiD. ed. Piccin, Padova, 1977.
21.
WolbergW. H.: Inhibition of migration of human autogenous and allogeneic leukocytes by extracts of patients cancers. Cancer Res., 31: 798–802, 1971.
