A murine monoclonal antibody (MAb) 2G3 of the lgG1 type was raised using the human esophageal squamous cell carcinoma (SCC) cell line TE-2. Immunoblotting with 2G3 indicated that the antigen recognized by 2G3 has a molecular weight of 34 kD. Its activity was evaluated by immunoperoxidase and immunofluorescence on frozen and paraffin sections of various normal tissues, normal and benign tumors as well as various established cell lines. The pattern of reactivity revealed that the antigen recognized by 2G3 was expressed mainly by esophageal SCC. The only exception was represented by malignant breast tumors, where it reacted weakly. Scatchard analysis using 125I-labelled 2G3 showed that TE-2 has approximately 7.5 times more binding sites than the MCF-7 breast cancer cell line. The use of this new MAb is therefore proposed for the histopathological diagnosis of esophageal SCC.
BernalS.D., SpeakJ.A.: Membrane antigen in small-cell carcinoma of the lung defined by monoclonal antibody SM1.Cancer Res., 44: 256–270, 1984.
2.
BoeheimK., SpeakJ.A., FreiE., BernalS.D.: SQMl antibody the head and neck.Int. J. Cancer, 36: 137–142, 1985.
3.
BourneJ.A.: Immunochemistry.In: Laboratory handbook of immunoperoxidase staining methods.DAKO Corporation, California, USA, 1983.
4.
CordellJ., RichardsonT., PulfordK., GhoshA., GatterK., HeydermanE., MasonD.: Production of monoclonal antibodies against human epithelial membrane antigen for use in diagnostic immunocytochemistry.Br. J. Cancer, 52: 347–354, 1985.
5.
DouillardJ.Y., HoffmanT., HerbermanR.B.: Enzyme linked immunosorbant assay for screening monoclonal antibody production: use of intact cells as antigen.J. Immunol. Methods, 39: 309–319, 1980.
6.
FernsteinP.D., PeknyK.W., ReisfeldR.A., WalkerL.E.: Antigens associated with human squamous-cell lung carcinoma defined by murine monoclonal antibodies.Cancer, 46: 2970–2977, 1986.
7.
FranekF.: Purification of 1gG monoclonal antibodies from ascitic fluid based on Rivanol precipitation.Methods in En-zymology, 121: 633–638, 1986.
8.
GillG.W.: The Shandon Cytospin (2) in diagnostic cytology.Technique, Tips and Troubleshooting, March, 1982.
9.
GioanniJ., SamsonM., ZanghelliniE., MazeanC., EttoreF., DemardF., ChauvelP., DuplayH., ScheinderM., LaurentJ., LalanneC.: Characterization of a new surface epitope specific for human epithelial cells defined by a monoclonal antibody and application to tumour diagnosis.Cancer Res., 47; 4417–4424, 1987.
10.
GodingJ.: Production of monoclonal antibodies.In: Monoclonal antibodies, principles and practice, pp. 59–103, Academic Press, New York, USA, 1986.
11.
HanaiN., ShitaraK., YoshidaH.: Generation of monoclonal antibodies against human lung squamous-cell carcinoma and adenocarcinoma using mice rendered tolerant to normal human lung.Cancer Res., 46: 4438–4443, 1986.
12.
Hospital Cancer Registry, Annual Report 75-78, Tata Memorial Hospital, Bombay-400012, India, 1988.
13.
KimmelK.A., CareyT.E.: Altered expression in squamous carcinoma cells of an orientation-restricted epithelial antigen by monoclonal antibody A9.Cancer Res., 46: 3614–3623, 1986.
14.
KyoizumiS., AkiyamaM., KounoN., KobukeK., HakodaM., JonesS., YamakidoM.: Monoclonal antibodies to human squamous-cell carcinoma of the lung and their application to tumor diagnosis.Cancer Res., 45: 3274–3281, 1985.
15.
LaemmliU.K.: Cleavage of strucural proteins during the assembly of the bacteriophage T4.Nature, 227: 680–685, 1970.
16.
LibertiP.A., HackettC.J., AskonasB.A.: Influenza virus infection of mouse lymphoblasts alters the binding affinity of anti-H-2 antibody: requirement for viral neuraminidase.Eur. J. Immunol., 9: 751–757, 1979.
17.
LowryO.H., RosebroughN.J., FarrA.L., RandallR.J.: Protein measurement with folin phenol reagent.J. Biol. Chem., 193: 265–271, 1951.
18.
MarkwellM.K., FoxC.F.: Surface-specific iodination of membrane proteins of viruses and eukaryotic cells using 1, 3, 4, 6-tetrachloro-3a,6a-diphenylglycoluril.Biochemistry, 17: 4807–4817, 1978.
19.
MollerG.: Demonstration of mouse isoantigens at cellular level by fluorescence antibody technique.J. Exp. Med., 114: 415–434, 1961.
20.
MulshineJ.L., CultittaF., BibroM., FedorkoJ., FargionS., LittleC., CarneyD., GazdarA., MinnaJ.: Monoclonal antibodies that distinguish non-small cell from small cell lung cancer.J. Immunol., 131: 497–502, 1983.
21.
NishiharaT., KasaiM., MoriS., WatanabeT., KuriyaY., SudaM., KitamuraM., HirayamaK., AkaishiT., SaskiT.: Characteristics of two cell lines (TE-1 and TE-2) derived from human squamous cell carcinoma of esophagus.Gann, 70: 575–582, 1979.
22.
QuakJ.J., BalmF.J.M., BrakkeeJ.G.P., ScheperR.J., MeijerC.J., SnowG.B.: Production of monoclonal antibodies to squamous-cell carcinoma antigens.Arch. Otolaryngol. Head Neck Surg., 116: 181–185, 1990.
23.
RosaiJ.: Ackerman's surgical pathology, 7th edition, pp. 467–486, C.V. Mosby Co., Missouri, USA, 1989.
24.
StahelR.A., SpeakJ.A., BernaiS.D.: Murine monoclonal antibody LAM2 defines cell membrane determinant with preferential expression on human lung small-cell carcinoma and squamous cell carcinomas.Int. J. Cancer, 35: 11–17, 1985.
25.
TatakeR.J., AminK.M., ManiarH.S., JambhekarN.A., ShrikhandeS.S., GangalS.G.: Monoclonal antibody against human squamous-cell carcinoma-associated antigen.Int. J. Cancer., 44: 840–845, 1989.
26.
TowbinH., StaehelinT., GordonJ.: Electrophoretic transfer of proteins from Polyacrylamide gels to nitrocellulose sheets: Procedures and some applications.Proc. Natl. Acad. Sci. USA,76: 4350, 1979.
