The gross lesions in 23 young male Beagle dogs given daily oral doses of 0.2–3.0 mg/kg body weight ochratoxin A consisted of moderate to severe mucohemorrhagic enteritis of the cecum, colon, and rectum; tonsillitis: and enlargement of lymph nodes, which were edematous, hyperemic and focally necrotic. Histopathologic alterations in the kidneys consisted of necrosis and desquamation of epithelial cells mainly in, but not limited to, proximal convoluted tubules. Many proximal and distal convoluted tubules contained eosinophilic, granular casts. Necrosis of lymphoid tissues was a prominent feature of the mycotoxicosis and focally occurred in, but was not limited to, germinal centers in the spleen, tonsils, thymus, and lymph nodes and the lymphoid nodules of the jejunum, ileum, cecum, colon, rectum and nictitating membrane. Necrosis did not occur in bone marrow. Hepatic alterations consisted of slight to moderate centrilobular necrosis and fatty change and occurred mainly in dogs receiving 0.2–0.3 mg/kg body weight ochratoxin A daily for 11–14 days.
References
1.
ChoudhuryH.CarlsonC.W.SemeniukG.:
A study of ochratoxin toxicity in hens.
Poult. Sci.50:
1855–1859
(1971).
2.
ChuF.S.:
Interaction of ochratoxin A with bovine serum albumin.
Arch. Biochem. Biophys.147:
359–366
(1971).
3.
ChuF.S.ChangC.C.:
Sensitivity of chicks to ochratoxins.
J. Ass. off. anal. Chcm.54:
1032–1034
(1971).
4.
ChuF.S.NohI.ChangC.C.:
Structural requirements for ochratoxin intoxication.
Life Sci.11:
503–508
(1972).
5.
CornishH.H.BarthM.L.DodsonV.N.:
Isozyme profiles and protein patterns in specific organ damage.
Toxicol. appl. Pharmacol.16:
411–423
(1970).
DinmanB.D.FoxC.F.FrajolaW.J.RaborA.:
Serum enzyme and B12 changes in CCI4 hepatotoxicity.
Arch. environm. HIth4:
168–182
(1962).
8.
DosterR.C.SinnhuberR.O.:
Comparative rates of hydrolysis of ochratoxins A and B in vitro.
Fd. Cosmet. Toxicol.10:
389–394
(1972).
9.
DosterR.C.SinnhuberR.C.WalesJ.H.:
Acute intraperitoneal toxicity of ochratoxins A and B in rainbow trout (Salmo gairdneri), Fd.
Cosmet. Toxicol.10:
85–92
(1972).
10.
DoupnikB.PeckhamJ.C.:
Mycotoxicity of Aspergillus ochraceux to chicks.
Appl. Microbiol.19:
594–597
(1970).
11.
GopinathC.JonesR.S.FordE.J.H.:
The effect of the repeated administration of halothane on the liver of the horse.
J. Path.102:
107–114
(1970).
12.
GriceH.C.BarthM.L.CornishH.H.FosterG.V.GrayR.H.:
Correlation between serum enzymes, isozyme patterns and histologically detectable organ damage.
Fd. Cosmet. Toxicol.9:
847–855
(1971).
13.
HesseltineC.W.VandergraftE.E.FennellD.I.SmithM.L.ShotwellO.L.:
Aspergilli as ochratoxin producers.
Mycologia64:
539–550
(1972).
14.
HoeC.M.JabaraA.G.:
The use of serum enzymes as diagnostic aids in the dog.
J. comp. Path.77:
245–254
(1967).
15.
KlassenC.D.PlaaG. L.:
Relative effects of various chlorinated hydrocarbons on liver and kidney function in dogs.
Toxicol. appl. Pharmacol.10:
119–131
(1967).
16.
Koch-WeserD.FarberE.PopperH.:
Fatty liver with and without necrosis.
Arch. Path.51:
498–509
(1951).
17.
KramplV.:
Relationship between serum enzymes and histological changes in liver after administration of heptachlor in the rat.
Bull. envir. Contam. Toxic.5:
529–536
(1971).
18.
KroghP.:
The pathology of mycotoxicoses.
J. stored Prod. Res.5:
259–264
(1969).
19.
LiaM.SemeniukG.HesseltineC.W.:
Conditions for production of ochratoxin A by Aspergillus species in a synthetic medium.
Appl. Microbiol.19:
542–544
(1970).
20.
LunaL.G., (ed.):
Best's carmine method for glycogen, in
Manual of histologic staining methods of the Armed Forces Institute of Pathology;
3rd ed.,
pp. 157–158 (
McGraw-Hill,
New York1968).
21.
McMannusJ.F.A.MowryR.W.:
Staining methods — histologic and histochemical,
p. 123
(Harper & Row,
New York1965).
22.
MayerC. F.:
Endemic panmyelotoxicosis in the Russian grain belt.
Milit. Surg.113:
173–189
(1953).
23.
MunroI.C.MoodieC.A.MiddeletonE.J.ScottP.M.GriceH.C.:
Toxicity of ochratoxin A in the rat.
Toxicol. appl. Pharmacol.22:
111
(1972).
24.
PeckhamJ.CDoupnikB.JonesO.H.:
Acute toxicity of ochratoxins A and B in chicks.
Appl. Microbiol.21:
492–494
(1971).
25.
PitoutM. J.:
The effect of ochratoxin A on glycogen storage in the rat liver.
Toxicol. appl. Pharmacol.13:
299–306
(1968).
26.
PurchaseI.F.H.NelW.:
Recent advances in research on ochratoxin. I. Toxicological aspects; in
Matales and Wogan Biochemistry of some foodborne microbial toxins,
pp. 153–156 (
MIT Press.
Cambridge1967).
27.
Purchase;I.F.H.TheronJ.J.:
The acute toxicity of ochratoxin A to rats.
Fd. Cosmet Toxicol.6:
479–483
(1968).
28.
PurchaseI.F.H.WattJ.J.van der:
The long-term toxicity of ochratoxin A to rats.
Fd. Cosmet. Toxicol.9:
681–682
(1971).
29.
SteynP.S.HolzapfelC.W.:
The isolation of the methyl and ethyl esters of ochratoxins A and B, metabolites of Aspergillus ochraceus Wilh.
J. sth afr. chem. Inst.20:
186–189
(1967).
30.
SzczechG.M.CarltonW.W.TuiteJ.:
Ochratoxicosis in Beagle dogs I. Clinical and clinical pathologic features.
Vet. Path.10:
135–154
(1973).
31.
SzczechG. M.CarltonW. W.TuiteJ.:
Ochratoxicosis in swine.
Vet. Path.
(in press).
32.
TheronJ.J.MerweK.J.van derLiebenbergN.JoubertH.J.B.NelW.:
Acute liver injury in ducklings and rats as a result of ochratoxin poisoning.
J. Path. Bact.91:
521–529
(1966).
33.
MerweK.J.van derSteynP.S.FourieL.ScottB.deTheronJ.J.:
Ochratoxin A, a toxic metabolite produced by Aspergillus ochraceus Wilh.
Nature, Lond.205:
1112–1113
(1965).
34.
WalbeekW.vanScottP.M.HarwigJ.LawrenceJ.W.:
Penicillium viridicatum Westling: a new source of ochratoxin A.
Canad. J. Microbiol.15:
1281–1285
(1969).
