Electron microscopic studies of bovine kidneys with amyloidosis showed macrophage-like cells in the interstitial amyloid that were either disseminated or in small groups. They had rather uniform protracted vacuoles filled with parallel fibrils. Similar vacuoles were also found in some reticular interstitial cells. In some samples electron-dense vacuoles and vacuoles containing fibrils were seen. Protracted lysosomal enzyme activity was seen in macrophage-like cells and reticular interstitial cells; these areas of activity showed green birefringence with Congo red. The vacuoles were thought to be lysosomes, and the parallel amyloid fibrils could be a consequence of intracellular formation or of phagocytosis.
References
1.
BarkaT.AndersonP.:
Histochemical methods for acid-phosphatase using hexazonium pararosanilin as coupler.J. Histochem. Cytochem.10:
741–753(1962)
2.
BattagliaS.:
Elektronenoptische Untersuchungen am Leberamyloid der Maus.Beitr. path. Anat.126:
300–320(1962)
3.
BendittE. P.EriksenN.BerglundC.:
Congo red dichroism with dispersed amyloid fibrils, an extrinsic cotton effect.Proc. natn. Acad. Sci. USA66:
1044–1051(1970)
4.
Ben-IshayZ.ZlotnickA.:
The cellular origin of amyloid. Electron microscopic study in a case of amyloidosis.Israel J. med. Scis4:
987–994(1968)
5.
BlackM. M.:
The nature, pathogenesis and staining properties of amyloid.Br. J. Derm.87:
280–283(1972)
6.
BulgerR. E.TrumpB. F.:
Fine structure of the rat renal papilla.Am. J. Anat.118:
685–722(1966)
7.
BywatersE. G. L.:
Amyloidosis,
p. 80
(Excerpta Medica,
Amsterdam1968)
8.
CohenA. S.ShirahamaT.:
Ultrastructural studies of renal peritubular amyloid experimentally induced in guinea pigs.Arthritis Rheum.14:
429–439(1971)
9.
CohenA. S.ShirahamaT.:
Animal model. Spontaneous and induced amyloidosis.Am. J. Path.68:
441–444(1972)
10.
CohenA. S.ShirahamaT.:
Electron microscopic analysis of isolated amyloid fibrils from patients with primary, secondary and myeloma associated disease. A study utilizing shadowing and negative staining techniques.Israel J. med. Scis9:
849–856(1973)
11.
CrowellW. A.GoldstonR. T.SchallW. D.FincoD. R.:
Generalized amyloidosis in a cat.J. Am. vet. med. Ass.161:
1127–1133(1972)
12.
DingleI. T.FellH. B.:
Lysosomes in biology and pathology,
vol. 1
(North-Holland,
Amsterdam1969)
13.
FranklinE. C.Zucker-FranklinD.:
Current concepts of amyloid.Adv. Immunol.15:
249–304(1972)
14.
GruysE.:
Bovine renal amyloidosis. I. Incidence, macroscopical and microscopical features.Neth. J. vet. Sci.99:
715–728(1974)
15.
GueftB.KikkawaY.HirschlS.:
An electron-microscopic study of amyloidosis from different species; in
Amyloidosis,
pp. 172–182
(Excerpta Medica,
Amsterdam1968)
16.
IshiharaT.:
Experimental amyloidosis using silver nitrate. Electron microscopic study on the relationship between silver granules, amyloid fibrils and reticuloendothelial system.Acta path. jap.23:
439–464(1973)
17.
JaoW.PiraniC. L.:
Renal amyloidosis: electron microscopic observations.
Acta path. microbiol. scand.,
suppl. 233,
pp. 217–227(1972)
18.
KazimierczakJ.:
Ultrastructural observations on the first amyloid to be found in the spleen of casein-treated mice.
Acta path. microbiol. scand.,
suppl. 233,
pp. 141–150(1972)
19.
LendrumA. C.SliddersW.FraserD. S.:
Renal hyalin. A study of amyloidosis and diabetic fibrinous vasculosis with new staining methods.J. clin. Path.25:
373–396(1972)
RomhányiG.:
Selective differentiation between amyloid and connective tissue structures based on the collagen specific topo-optical staining reaction with Congo red.Virchows Arch. path. Anat. Physiol.354:
209–222(1971)
27.
ShimamuraT.MorrisonA. B.:
Vascular permeability of the renal medullary vessels in the mouse and rat.Am. J. Path.71:
155–166(1973)
28.
ShirahamaT.CohenA. S.:
Fine structure of the glomerulus in human and experimental renal amyloidosis.Am. J. Path.51:
869–912(1967)
29.
ShirahamaT.CohenA. S.:
Lysosomal breakdown of amyloid fibrils by macrophages.Am. J. Path.63:
463–485(1971)
30.
ShirahamaT.CohenA. S.:
An analysis of the close relationship of lysosomes to early deposits of amyloid. Ultrastructural evidence in experimental mouse amyloidosis.Am. J. Path.73:
97–115(1973)
31.
SorensonG. D.HeefnerW. A.KirkpatrickJ. B.:
Experimental amyloidosis. II. Light and electron microscopic observations of liver.Am. J. Path.44:
629–644(1964)
32.
SorensonG. D.BariW. A.:
Murine amyloid deposits and cellular relationships; in
Amyloidosis,
pp. 58–73.
(Excerpta Medica,
Amsterdam1968)
33.
YatesR. D.RigdonR. H.MascorroJ. A.:
Amyloid in the gastrointestinal tract of mice. An electron microscopic study.Tex. Rep. Biol. Med.31:
89–97(1973)
34.
Zucker-FranklinD.:
Immunophagocytosis of human amyloid fibrils by leucocytes.J. Ultrastruct. Res.32:
247–257(1970)
35.
Zucker-FranklinD.FranklinE. C.:
Intracellular localization of human amyloid by fluorescence and electron microscopy.Am. J. Path.59:
23–41(1970)
