Chickens were housed when 1 day old with chickens experimentally infected with Mycoplasma synoviae. Air sacs from 16 of the 2-week-old and eight of the 3-week-old contact-exposed chickens were given gross lesion scores and embedded in glycol methacrylate for slide preparation. Histologic lesions in air sacs with gross scores of 0–2 were mild edema resulting in a two to eightfold increase in air sac thickness, capillary proliferation, and exudate consisting largely of heterophils and necrotic debris. Histologic lesions in air sacs with gross scores of 3 and 4 were marked hyperplasia of epithelial cells and diffuse infiltration of the air sac connective tissue by mononuclear cells. Nine of 10 air sacs with gross scores of 0 had no histologic evidence of inflammation. The most severe histologic lesions were in those air sacs with gross scores of 4. The glycol methacrylate procedure resulted in 2-μm sections with excellent cellular detail.
References
1.
AdlerH. E.PageL. A.:
Haemophilus infections in chickens. II. The pathology of the respiratory tract.Avian Dis.6:
1–6(1961)
2.
AdlerH. E.McMartinD. A.OrtmayerH.:
The effect of infectious bronchitis virus on chickens infected with Mycoplasma gallisepticum.Avian Dis.6:
267–274(1962)
3.
AryaP. L.SautterJ. H.PomeroyB. S.:
Pathogenesis and histopathology of airsacculitis in turkeys produced by experimental inoculation of day-old poults with Mycoplasma meleagridis.Avian Dis.15:
163–176(1971)
4.
BarberC. W.:
The lymphofollicular nodules in turkey tissues associated with Mycoplasma gallisepticum infection.Avian Dis.6:
289–296(1962)
5.
BellR. B.:
Histology of the local cellular response of ducks to injections of antigenic material.Reticuloendoth. Soc. Res. J.15:
213–224(1974)
6.
CarlsonH. C.AllenJ. R.:
The acute inflammatory reaction in chicken skin. Blood cellular response.Avian Dis.13:
817–833(1969)
7.
CarlsonH. C.BeggsE. C.:
Ultrastructure of the abdominal air sac of the fowl.Res. vet. Sci.14:
148–150(1973)
8.
ChalquestR. R.FabricantJ.:
Pleuropneumonia-like organisms associated with synovitis in fowls.Avian Dis.4:
515–539(1960)
9.
CoverM. S.:
Gross and microscopic anatomy of the respiratory system of the turkey. III. The air sacs.Am. J. vet. Res.14:
239–245(1953)
10.
FletcherO. J.:
Plastic embedding of avian tissues for diagnostic histopathology.Avian Dis.19:
201–208(1975)
11.
GhazikhanianG.YamamotoR.CordyD. R.:
Response of turkeys to experimental infection with Mycoplasma synoviae.Avian Dis.17:
122–136(1973)
12.
GhazikhanianG.YamamotoR.:
Characterization of pathogenic and nonpathogenic strains of Mycoplasma meleagridis.Manifestations of disease in turkey embryos and poults. Am. J. vet. Res.35:
417–424(1974)
13.
GrimesT. M.RosenfeldL. E.:
Experimental respiratory disease and airsacculitis in fowls caused by Mycoplasma gallisepticum.Aust. vet. J.48:
113–116(1972)
14.
GrossW. B.:
Pathological changes of an Escherichia coli infection in chickens and turkeys.Am. J. vet. Res.18:
724–730(1957)
15.
ItakuraC.:
Erosion and its subsequent lesion in fowl air sacs.Jap. J. vet. Res.30:
15–19(1968)
16.
JohnsonE. P.:
The specificity of lymphofollicular lesions in the diagnosis of chronic respiratory disease.Cornell Vet.44:
230–239(1954)
17.
JortnerB. S.AdamsW. R.:
Turpentine-induced inflammation in the chicken.A light- and electron-microscope study, with emphasis on the macrophage, epithelioid cells, and multinucleated giant cell reaction. Avian Dis.15:
533–550(1971)
18.
JungherrE.MinardE. L.:
The pathology of experimental avian pneumoencephalitis.Am. J. vet. Res.5:
125–134(1944)
19.
JungherrE.LuginbühlR. E.:
Air sac infection in poultry.
Proc. 56th Ann. Meeting US Livestock Sanitary Ass., Louisville
1952, pp. 275–283
20.
JungherrE.LuginbühlR. E.JacobsR. E.:
Pathology and serology of air sac infection.
Proc. 90th Ann. Meeting Am. vet. med. Ass., Toronto
1953,
pp. 303–312
21.
KerrK. M.OlsonN. O.:
Pathology in chickens experimentally inoculated or contact-infected with Mycoplasma gallisepticum.Avian Dis.11:
559–578(1967)
22.
KingD. D.KlevenS. H.WengerD. M.AndersonD. P.:
Field studies with Mycoplasma synoviae.Avian Dis.17:
722–726(1973)
23.
KlevenS. H.KingD. D.AndersonD. P.:
Airsacculitis in broilers from Mycoplasma synoviae.Effect on air sac lesions of vaccinating with infectious bronchitis and Newcastle virus. Avian Dis.16:
915–924(1972)
24.
KlevenS. H.FletcherO. J.DavisR. B.:
Influence of strain of Mycoplasma synoviae and route of infection on development of synovitis or airsacculitis in broilers.Avian Dis.19:
126–135(1975)
25.
LucasA. M.DeningtonE. M.:
A brief report on anatomy, histology, and reactivity of air sacs in the fowl.Avian Dis.5:
460–461(1961)
26.
LucasA. M.DeningtonE. M.:
Air sacs. Their distribution and microscopic structure.ARS Bull.45–2:
32–38(1961)
27.
MoorheadP. D.SaifY. M.:
Mycoplasma meleagridis and Escherichia coli infections in germ-free and specific-pathogen-free turkey poults.Pathologic manifestations. Am. J. vet. Res.31:
1645–1653(1970)
28.
NairM. K.:
The early inflammatory reaction in the fowl.
Acta vet. scand.,
suppl. 42,
pp. 1–103(1973)
29.
OlsonN. O.KerrK. M.CampbellA.:
Control of infectious synovitis.13. The antigen study of three strains. Avian Dis.8:
209–214(1964)
30.
PurcellD. A.:
Histopathology of infectious laryngotracheitis in fowl infected by an aerosol.J. comp. Path.81:
421–431(1971)
31.
RhoadesK. R.:
Mycoplasma meleagridis infection.Development of air-sac lesions in turkey poults. Avian Dis.15:
910–922(1971)
32.
SiccardiF. J.:
Mycoplasma synoviae airsacculitis, laboratory reproduction, characterization, and comparison with field specimens.
Proc. Mycoplasma Workshop, New Orleans. Mycoplasmosis Committee AAAP,
pp. 22–23(1972)
33.
TaliaferroW. H.BloomW.:
Inflammatory reactions in the skin of normal and immune canaries and monkeys after the local injection of malarial blood.J. infect. Dis.77:
109–138(1945)
34.
TruscottR. B.FergusonA. E.RuhnkeH. L.PettitJ. R.RobertsonA.SpeckmannG.:
An infection in chickens with a strain of Mycoplasma gallisepticum of low virulence.Can. J. comp. Med.38:
341–343(1974)
35.
YamamotoR.BiglandC. H.:
Experimental production of airsacculitis in turkey poults by inoculation with ‘N’-type mycoplasma.Avian Dis.9:
108–118(1965)
