Necropsy findings in adult salmon with subclinical “Hitra disease” (hemorrhagic syndrome) varied from negligible to hemorrhagic diathesis with generalized edema. By light microscopy, minute vessels were dilated, arterioles had mural necrosis, and thrombi were present. Occlusions consisted of fused erythrocytes and fibrin. Ultrastructurally, endothelial nuclei were pyknotic with condensed chromatin. The cytoplasm contained numerous laminar structures and electron-dense particles. Damaged erythrocytes adhered to the degenerated endothelium, and coalesced erythrocytes had formed mural thrombi or filled the vascular lumen. Microthrombi were frequently seen. There were no thrombocytes in the thrombi, which consisted of erythrocytes or reticulocytes. Clinically healthy farmed salmon parr showed the same disorders as did adults, but to a far lesser degree. Wild salmon parr had normal microvasculature. Results indicate that disseminated intravascular coagulation (DIC) occurs in “Hitra disease” in farmed Atlantic salmon.
References
1.
BercziIBertókLBereznaiT:
Comparative studies on the toxicity of E. coli lipopolysaccharide endotoxin in various animal species.Can J Microbiol12:
1070–1071,
1966
2.
BrunoDWHastingsTSEllisAEWoottenR:
Outbreak of a cold water vibriosis in Atlantic salmon in Scotland.Bull Eur Assoc Fish Pathol5:
62–63,
1985
3.
CassilasESmithLSWoodwardJJD'AoustBG:
Hematologic response of fish to changes in gas or hydrostatic pressure.Am J Physiol239:
161–167,
1980
4.
CohenWD:
Observations on the marginal band system of nucleated erythrocytes.J Cell Biol78:
260–273,
1978
5.
DaleJOhlssonKNorstogaKAasenAO:
Intravascular hemolysis and ultrastructural changes of erythrocytes in lethal canine endotoxin shock.Eur Surg Res12:
39–51,
1980
6.
EgidiusEAndersenKClausenERaaJ:
Coldwater vibriosis or “Hitra disease” in Norwegian salmonid farming.J Fish Dis4:
353–354,
1981
7.
EgidiusESoleimØAndersenK:
Further observations on cold-water vibriosis or Hitra disease.Bull Eur Assoc Fish Pathol4:
50–51,
1984
8.
EllisAE:
The leucocytes of fish: a review.J Fish Biol11:
453–491,
1977
9.
EllisAE:
The anatomy and physiology of teleosts.In:
Fish Pathology,
ed. RobertsRJ,
pp. 13–54.
Bialliere Tindall,
London,
1978
10.
FawcettDWWitebskyF:
Observations on the ultrastructure of nucleated erythrocytes and thrombocytes, with particular reference to the structural basis of their discoidal shape.Z Zellforsch62:
785–806,
1964
11.
FinstadBStaurnesMReiteOB:
Effekt av temperatur på sj⊘vannstoleranse hos regnbue⊘rret.Nor Fiskeoppdr10:
44–45,
1985
12.
FinstadB:
Effekt av temperatur på sy⊘vannstoleranse hos regnbue⊘rret Salmo gairdneri,
p. 60.
Cand Scient Thesis, University of Trondheim,
1986
13.
Fj⊘lstadMHeyeraasAL:
Muscular and myocardial degeneration in cultured Atlantic salmon, Salmo salar L., suffering from “Hitra disease.”J Fish Dis8:
367–372,
1985
14.
FreemanBACrapoJD:
Biology of disease. Free radicals and tissue injury.Lab Invest47:
412–426,
1982
15.
HarbellSCHodginsHOSchieweMH:
Studies on the pathogenesis of vibriosis in coho salmon Oncorhyncus kisutch (Walbaum).J Fish Dis2:
391–404,
1979
16.
HochsteinPJainSKRice-EvansC:
The physiological significance of oxidative perturbations in erythrocyte membrane lipids and protein.
In: The Red Cell, Fifth Ann Arbor Conference,
pp. 449–459.
AR Liss Inc, New York,
1981
17.
HolmKOStr⊘mEStensvågKRaaJJ⊘rgensenT:
Characteristics of a Vibrio sp. associated with the “Hitra disease” of Atlantic salmon in Norwegian fish farms.Fish Pathol20:
125–129,
1985
18.
LeightonFA:
Morphological lesions in red blood cells from Herring Gulls and Atlantic Puffins ingesting Prudhoe Bay crude oil.Vet Pathol22:
393–402,
1985
19.
LerayCNonnotteGNonnotteL:
The effect of dietary lipids on the trout erythrocyte membrane.Fish Physiol Biochem1:
27–35,
1986
20.
MaxieMG:
The vascular system.In:
Pathology of domestic animals,
ed. JubbKVKennedyPCPalmerN3rd ed.,
vol. 3,
pp. 34–81.
Academic Press,
Orlando,
1985
21.
McKayDG:
Disseminated Intravascular Coagulation. An Intermediary Mechanism of Disease.
Harper and Row,
New York,
1965
22.
MillerTK:
Coagulation changes in rainbow trout experimentally infected with Yersinia ruckeri: a possible role of endotoxin.
Abstr 13th Conf Int Assoc Aquatic Anim Med,
1982
23.
MillerTKLevinJ:
The effects of multiple injections of bacterial endotoxin on blood coagulation in the toadfish, Opsanus tau.Biol Bull166:
109–205,
1984
24.
MocciaRDHungSSOSlingerSJFergusonHW:
Effect of oxidized fish oil, vitamin E and ethoxyquin on the histopathology and haematology of rainbow trout Salmo gairdneri Richardson.J Fish Dis7:
269–282,
1984
25.
MooreDJ:
Disseminated intravascular coagulation: a review of its pathogenesis, manifestations and treatment.J S Afr Vet Assoc50:
259–264,
1979
26.
NafstadI:
Current knowledge of vitamin E. Biochemical functions and significance in diseases.
In: Norges Veterinærh⊘gskole Publ 1982,
pp. 5–14.
Oslo,
1983
27.
NordstogaK:
Porcine salmonellosis: a counterpart to the generalized Shwartzman reaction. Origin of hyaline material precipitated in minute vessels.Acta Pathol Microbiol Scand Sect A82:
690–702,
1974
28.
PoppeTTFr⊘slieAKoppangNHåsteinT:
Muskeldegenerasjon med eksudativ hemorrhagisk diatese hos oppdrettslaks—“Hitrasjuke.”Nor Vet Tidskr97:
159–165,
1985
29.
PoppeTTHåsteinTSalteR:
“Hitra Disease” (haemorrhagic syndrome) in Norwegian salmonid farming.In:
Fish and Shellfish Pathology,
ed. EllisAE,
pp. 223–229.
Academic Press,
London,
1985
30.
SalteR:
Karskader hos laks.aktuelt fra Statens fagtjeneste for landbruket6:
323–324,
1986
31.
SchwartzK:
Essentiality and metabolic functions of selenium.Med Clin North Am60:
745–758,
1976
32.
SolheimØ:
Kidney histology of Atlantic salmon (Salmo salar) suffering from cold-water vibriosis or Hitra disease.Bull Eur Assoc Fish Pathol5:
88–91,
1985
33.
StefaniniM:
Diffuse intravascular coagulation: an analysis of a basic mechanism of disease.Crit Rev Clin Lab Sci3:
349–372,
1972
34.
ThomsonRG:
General Veterinary Pathology,
2nd ed.,
pp. 100–162.
WB Saunders Co,
Philadelphia,
1984
35.
ThorgeirssonGRobertsonALJr:
The vascular endothelium—pathobiologic significance: a review.Am J Pathol93:
803–848,
1978
36.
ZapataACarratoA:
Ultrastructure of elasmobranch and teleost erythrocytes.Acta Zool62:
129–135,
1981
