Alterations in Pulmonary Morphology and Peripheral Coagulation Profiles Caused by Intratracheal Inoculation of Live and Ultraviolet Light-killed Pasteurella haemolytica A1 in Calves
Free accessResearch articleFirst published online July, 1991
Alterations in Pulmonary Morphology and Peripheral Coagulation Profiles Caused by Intratracheal Inoculation of Live and Ultraviolet Light-killed Pasteurella haemolytica A1 in Calves
Eighteen male Holstein calves were divided into groups of three and inoculated intratracheally with 5 x 109 logarithmic phase or ultraviolet light-killed Pasteurella haemolytica biotype A serotype 1. Serial coagulation profiles were done on one calf from each group during the first 24 hours after inoculation. One calf from each group was necropsied at 4, 12, and 24 hours after inoculation and lesions were characterized with light and transmission electron microscopy. We found that 1) the pulmonary intravascular macrophage may have an important role in the early intravascular inflammatory events; 2) there was morphologic evidence for local initation of the coagulation cascade in the lung early in the disease process but it was not a consumptive process; and 3) killed-bacteria were capable of causing fibrin exudation, platelet aggregation and alveolar epithelial damage similar to live bacteria, but the degenerative changes in neutrophils, endothelial cells and intravascular fibrin formation that occur with live bacteria were not seen.
AmesTRMarkhamRJFOpuda-AsiboJLeiningerJRMaheswaranSK:
Pulmonary response to intratracheal challenge with Pasteurella haemolytica and Pasteurella multocida.
Can J Comp Med49:
395–400,
1985.
2.
BabiukLALawmanMJPBiolMIGiffordGA:
Bovine respiratory disease: pathogenesis and control by interferon.
In:A Seminar In Bovine Immunology, Western Veterinary Conference,
pp. 12–24.
Veterinary Learning Systems Co Inc.LasVegas, NV,
1987.
3.
BalkwillFRBurkeF:
The cytokine network.
Immunol Today10:
299–304,
1989.
4.
BaluyutCSSimonsonRRBemrickWJMaheswaranSK:
Interaction of Pasteurella haemolytica with bovine neutrophils: identification and partial characterization of a cytotoxin.
Am J Vet Res42:
1920–1926,
1981.
5.
BertramTA:
Quantitative morphology of peracute pulmonary lesions in swine induced by Haemophilus pleuropneumoniae.
Vet Pathol22:
598–609,
1985.
6.
BertramTA:
Intravascular macrophages in lungs of pigs infected with Haemophilus pleuropneumoniae.
Vet Pathol23:
681–691,
1986.
7.
BertramTAOverbyLHDanilowiczRElingTEBrodyAR:
Pulmonary intravascular macrophages metabolize arachidonic acid in vitro.
Am Rev Respir Dis138:
936–944,
1988.
8.
BillinghamMEJ:
Cytokines as inflammatory mediators.
Br Med Bull43:
350–370,
1987.
9.
BohsCTFishJCMillerTHTraberDL:
Pulmonary vascular response to endotoxin in normal and lymphocyte depleted sheep.
Circ Res6:
13–21,
1979.
10.
BrogdenKAAdlamCLehmkuhlHDCutlipRCKnightsJMEngenRL:
Effects of Pasteurella haemolytica (AI) capsular polysaccharide on sheep lung in vivo and on pulmonary surfactant in vitro.
Am J Vet Res50:
555–559,
1989.
11.
ChangYFRenshawHWMartensRJLivingstonLW:
Pasteurella haemolytica leukotoxin: chemiluminescent response of peripheral blood leukocytes from several different mammalian species to leukotoxin and opsinintreated living and killed, Pasteurella haemolytica and Staphylococcus aureus.
Am J Vet Res47:
67–74,
1986.
12.
ChapmanAHStahlMAllenCLYeeRFairDS:
Regulation of the procoagulant activity within the bronchoalveolar compartment of normal human lung.
Am Rev Respir Dis137:
1417–1425,
1987.
13.
ClinkenbeardKDMosierDAConferAW:
Transmembrane pore size and role of cell swelling in cytotoxicity caused by Pasteurella haemolytica leukotoxin.
Infect Immun57:
420–425,
1989.
14.
ClinkenbeardKDMosierDATimkoALConferAW:
Effects of Pasteurella haemolytica leukotoxin on cultured bovine lymphoma cells.
Am J Vet Res50:
271–275,
1989.
15.
ConferAWPancieraRJMosierDA:
Bovine pneumonic pasteurellosis: immunity to Pasteurella haemolytica.
J Am Vet Med Assoc193:
1308–1313,
1988.
16.
CotranRS:
New roles for endothelium in inflammation and immunity.
Am J Pathol129:
407–413,
1987.
17.
DyerRM:
The bovine respiratory disease complex: a complex interaction of host, environment and infectious factors.
Compend Cont Educ4:
S296–S304,
1982.
FitschenCSandhausRAWorthenGSHansonPM:
Bacterial lipopolysaccharide enhances chemoattractant-induced clastasc secretion by human neutrophils.
J. Leukoc Biol43:
547–556,
1988.
FreudenbergMAFreudenbergNGalanosC:
Time course of cellular distribution of endotoxin in liver, lung and kidney of rats.
Br J Exp Pathol63:
56–65,
1982.
23.
FreudenbergNFreudenbergMAGuzmanJMittermayerCHBandaraKGalanosC:
Identification of endotoxin-positive cell in the rat lung during shock.
Virchows Arch [A]404:
197–211,
1984.
24.
FriendSCThomsonRGWilkieBN:
Pulmonary lesions induced by Pasteurella haemolytica in cattle.
Can J Comp Med41:
219–223,
1977.
25.
GartnerSLSiekmannDGKangYHWatsonLPHomerLD:
Effects of lipopolysaccharide, lipid A, lipid X and phorbol esters on cultured bovine endothelial cells.
Lab Invest59:
181–191,
1988.
26.
HaddenJWGalyAHaddenEMJouraineJLCoffexRG:
Cyclic nucleotides in the immunopharmacology of lipopolysaccharide endotoxins.
In:Immunobiology and Immunopharmacology of Bacterial Endotoxins,
ed. SzentivanyAFriedmanHNowotnyA,
pp. 187–200.
Plenum Press,
New York, NY,
1986.
27.
HammerRFWeberAF:
Ultrastructure of agranular leukocytes in peripheral blood of normal cows.
Am J Vet Res35:
527–536,
1974.
28.
HeffnerJESahnSARepineJE:
The role of platelets in the adult respiratory distress syndrome.
Am Rev Respir Dis135:
482–492,
1987.
29.
KachlerKLMarkamRJFMuscoplatCCJohnsonDW:
Evidence of species specificity in cytocidal effects of Pasteurella haemolytica,
Infect Immun30:
615–616,
1980.
30.
KiessREWillBHCollierJR:
Skin toxicity and hemodynamic properties of endotoxin derived from Pasteurella haemolytica.
Am J Vet Res25:
935–941,
1964.
31.
LandoPAEdgingtonTS:
Lymphoid procoagulant response to bacterial endotoxin in the rat.
Infect Immun50:
660–666,
1985.
32.
LoRYCStrathdeeGAShewenPE:
Nucleotide sequency of the leukotoxin genes of Pasteurella haemolytica A1.
Infect Immun55:
1987–1996,
1987.
33.
LopezAMaxicMGRuhnkeLSavanMThomasonRG:
Cellular inflammatory response in the lungs of calves exposed to bovine viral diarrhea virus, Mycoplasma bovis, and Pasteurella haemolytica.
Am J Vet Res47:
1283–1286,
1986.
34.
MartinSWMechAHDavisDGJohnsonJACurtisRA:
Factors associated with morbidity and mortality in feedlot calves: the Bruce county beef project year two,
Can J Comp Med45:
102–112,
1981.
35.
MeyrickBORyanUSBrighamKL:
Direct effects of Escherichia coli endotoxin on structure and permeability of pulmonary endothelial monolayers and the endothelial layer of intimal explants.
Am J Pathol122:
140–151,
1986.
36.
MorrsionDCUlevichRJ:
The effects of bacterial endotoxin on host mediator systems.
Am J Pathol93:
527–617,
1978.
37.
NathanCF:
Neutrophil activation on biological surfaces. Massive secretion of hydrogen peroxide in response to products of macrophages and lymphocytes.
J Clin Invest80:
1550–1560,
1987.
38.
OtulakowskiGLShewenPEUdohAEMellorsAWilkieBN:
Proteolysis of sialoglycoprotein by Pasteurella haemolytica cytotoxic culture supernate.
Infect Immun42:
64–70,
1983.
39.
PancieraRJCorstvetREConferAWGreshamCN:
Bovine pneumonic pasteurellosis: effects of vaccination with live Pasteurella species.
Am J Vet Res45:
2538–2542,
1984.
40.
ParkerCW:
Lipid mediators produced through the lipoxygenase pathway.
Ann Rev Immunol5:
65–84,
1987.
41.
RehmtullaAJThomsonRG:
A review of the lesions of shipping fever of cattle.
Can Vet J22:
1–8,
1981.
42.
RimsayRLCoyle-DennisJELauermanLHSquirePG:
Purified and biological characterization of endotoxin fractions from Pasteurella haemolytica.
Am J Vet Res42:
2134–2138,
1981.
43.
SandborgRRSmolenJE:
Early biochemical events in leukocyte activation.
Lab Invest59:
300–320,
1988.
44.
SchieferBWardGEMoffattRE:
Correlation of microbiological and histological findings in bovine fibrinous pneumonia.
Vet Pathol15:
313–321,
1978.
45.
ShewenPEWilkieBN:
Cytotoxin of Pasteurella haemolytica action on bovine leukocytes.
Infect Immun35:
91–94,
1982.
46.
SlocombeRFDerksinFJRobinsonNETrappAGuptaANewmanJP:
Interaction of cold stess and Pasteurella haemolytica in the pathogenesis of pneumonic pasteurellosis in calves: methods of induction, and hematologic and pathologic changes.
Am J Vet Res45:
1757–1763,
1984.
47.
SlocombeRFMarlarkJIngersollRDerksenFJRobinsonNE:
Importance of neutrophils in the pathogenesis of pneumonic pasteurellosis in calves.
Am J Vet Res46:
2253–2258,
1985.
48.
SternDMBankINawrothPPCassimerisJKisielWFentonJWDinarelloCChessLJaffeEA:
Self regulation of procoagulent events on the endothelial cell surface.
J Exp Med162:
1223–1235,
1985.
49.
TabatabaiLBFankGH:
Neuraminidase from Pasteurella haemolytica.
Curr Microbiol5:
203–206,
1981.
50.
UragohKSueishiKNakamuraTIwanagasS:
A novel immunohistochemical method for the in vivo detection of endotoxin using horseshoe crab factor C.
J Histochem Cytochem36:
1275–1283,
1988.
51.
WarnerAEDeCampMMMolinaRMBrainJD:
Pulmonary removal of circulating endotoxin results in acute lung injury in sheep.
Lab Invest59:
219–230,
1988.
52.
WarnerAEMolinaRMBrainJD:
Uptake of blood-borne bacteria by pulmonary intravascular macrophages and consequent inflammatory response in sheep.
Am Rev Respir Dis136:
683–690,
1987.
53.
WhiteleyLOMaheswaranSKWeissDJAmesTR:
Morphologic and morphometric analysis of the acute responce of the bovine alveolar wall to Pasteurella haemotytica A1-derived endotoxin and leukotoxin.
J Comp Pathol(in press).
54.
WhiteleyLOMaheswaranSKWeissDJAmesTR:
Immunohistochemical localization of Pasteurella haemolytica Al-derived endotoxin, leukotoxin and capsular polysaccharide in experimental bovine pasteurella pneumonia.
Vet Pathol27:
141–149,
1990.
55.
WerbZ:
How the macrophage regulates its extracellular environment.
Am J Anat166:
237–256,
1983.
56.
WikseSE:
Feed lot cattle pneumonia.
Vet Clin North Am [Food Anim Pract]1:
289–310,
1985.
57.
WorthenGSHaslettCSmedlyLAReesAJGumbayRSHensenJEHensonPM:
Lung vascular injury induced by chemotactic factors: enhanced by bacterial endotoxins.
Fed Proc45:
7–11,
1986.
58.
YatesWDG:
A review of infectious bovine rhinotracheitis shipping fever pneumonia and viral-bacterial synergism in respiratory disease of cattle.
Can J Comp Med46:
225–263,
1982.
