Effect of Increased Excursion of the Ankle on the Severity of Acute Eccentric Contraction-Induced Strain Injury in the Gastrocnemius

Abstract

Background: Although muscle strain injury models are frequently employed, highly invasive procedures, including surgical intervention, are typically used to produce these models. In this study, a minimally invasive model of acute strain injury was produced with a single eccentric contraction.

Hypothesis: The authors tested whether the severity of strain injury in the in vivo gastrocnemius depends on the excursion of the ankle.

Study Design: Controlled laboratory study.

Methods: Animals were randomly assigned to 2 groups: a small range of motion group (90°-105°, n = 10) and a large range of motion group (90°-125°, n = 10). Tetanically activated gastrocnemius muscles with percutaneous electrical stimulation were simultaneously elongated by forced dorsiflexion of the ankle joint.

Results: Isometric tetanic force of the large range of motion group was significantly lower than the pretreatment control 1, 2, and 3 days after treatment. Such reduction was not observed in the small range of motion group. Apparent pathologic damage was observed in the large range of motion group throughout the study period of 10 days.

Conclusions: A larger range of motion causes more damage in eccentric contraction by using the in vivo model.

Clinical Relevance: This in vivo model is useful to elucidate mechanisms of prevention and recovery of strain injury.

Keywords

muscle strain eccentric force deficit gastrocnemius

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References

Armstrong RB

Ogilvie RW

Schwane JA.

Eccentric exercise-induced injury to rat skeletal muscle. J Appl Physiol. 1983;54:80-93.

Best TM

McCabe RP

Corr D

et al.

Evaluation of a new method to create a standardized muscle stretch injury. Med Sci Sports Exerc. 1998;30:200-205.

Bianchi S

Martinoli C

Abdelwahab IF

et al.

Sonographic evaluation of tears of the gastrocnemius medial head (“tennis leg”). J Ultrasound Med. 1998;17:157-162.

Brooks SV

Zerba E

Faulkner JA.

Injury to muscle fibres after single stretches of passive and maximally stimulated muscles in mice. J Physiol. 1995;488:459-469.

Cottrell WC

Pearsall AW IV

Hollis MJ.

Simultaneous tears of the Achilles tendon and medial head of the gastrocnemius muscle. Orthopedics. 2002;25:685-687.

Delgado GJ

Chung CB

Lektrakul N

et al.

Tennis leg: clinical US study of 141 patients and anatomic investigation of four cadavers with MR imaging and US. Radiology. 2002;224:112-119.

Friden J

Sjostrom M

Ekblom B.

Myofibrillar damage following intense eccentric exercise in man. Int J Sports Med. 1983;4:170-176.

Garrett WE Jr.

Injuries to the muscle-tendon unit. Instr Course Lect. 1988;37:275-282.

Gordon AM

Huxley AF

Julian FJ.

Tension development in highly stretched vertebrate muscle fibres. J Physiol. 1966;184:143-169.

10.

Hasselman CT

Best TM

Seaber AV

et al.

A threshold and continuum of injury during active stretch of rabbit skeletal muscle. Am J Sports Med. 1995;23:65-73.

11.

Hesselink MK

Kuipers H

Geurten P

et al.

Structural muscle damage and muscle strength after incremental number of isometric and forced lengthening contractions. J Muscle Res Cell Motil. 1996;17:335-341.

12.

Hunter KD

Faulkner JA.

Pliometric contraction-induced injury of mouse skeletal muscle: effect of initial length. J Appl Physiol. 1997;82:278-283.

13.

Huxley AF

Peachey LD.

The maximum length for contraction in vertebrate striated muscle. J Physiol. 1961;156:150-165.

14.

Kawakami Y

Amemiya K

Kanehisa H

et al.

Fatigue responses of human triceps surae muscles during repetitive maximal isometric contractions. J Appl Physiol. 2000;88:1969-1975.

15.

Lieber RL

Schmitz MC

Mishra DK

et al.

Contractile and cellular remodeling in rabbit skeletal muscle after cyclic eccentric contractions. J Appl Physiol. 1994;77:1926-1934.

16.

Lieber RL

Woodburn TM

Friden J.

Muscle damage induced by eccentric contractions of 25% strain. J Appl Physiol. 1991;70:2498-2507.

17.

Lowe DA

Warren GL

Ingalls CP

et al.

Muscle function and protein metabolism after initiation of eccentric contraction-induced injury. J Appl Physiol. 1995;79:1260-1270.

18.

Lynch GS

Faulkner JA.

Contraction-induced injury to single muscle fibers: velocity of stretch does not influence the force deficit. Am J Physiol. 1998;275:1548-1554.

19.

Macpherson PC

Schork MA

Faulkner JA.

Contraction-induced injury to single fiber segments from fast and slow muscles of rats by single stretches. Am J Physiol. 1996;271:C1438-C1446.

20.

McCully KK

Faulkner JA.

Injury to skeletal muscle fibers of mice following lengthening contractions. J Appl Physiol. 1985;59:119-126.

21.

Millar AP.

Strains of the posterior calf musculature (“tennis leg”). Am J Sports Med. 1979;7:172-174.

22.

Newham DJ

Jones DA

Clarkson PM.

Repeated high-force eccentric exercise: effects on muscle pain and damage. J Appl Physiol. 1987;63:1381-1386.

23.

Newham DJ

Jones DA

Ghosh G

et al.

Muscle fatigue and pain after eccentric contractions at long and short length. Clin Sci. 1988;74:553-557.

24.

Nikolaou PK

Macdonald BL

Glisson RR

et al.

Biomechanical and histological evaluation of muscle after controlled strain injury. Am J Sports Med. 1987;15:9-14.

25.

Noonan TJ

Best TM

Seaber AV

Garrett WE Jr.

Identification of a threshold for skeletal muscle injury. Am J Sports Med. 1994;22:257-261.

26.

Noonan TJ

Garrett WE Jr.

Injuries at the myotendinous junction. Clin Sports Med. 1992;11:783-806.

27.

Orchard J

Best TM.

The management of muscle strain injuries: an early return versus the risk of recurrence. Clin J Sport Med. 2002;12:3-5.

28.

Orchard JW.

Intrinsic and extrinsic risk factors for muscle strains in Australian football. Am J Sports Med. 2001;29:300-303.

29.

Orchard JW

Alcott E

James T

et al.

Exact moment of a gastrocnemius muscle strain captured on video. Br J Sports Med. 2002;36:222-223.

30.

Speer KP

Lohnes J

Garrett WE Jr.

Radiographic imaging of muscle strain injury. Am J Sports Med. 1993;21:89-95.

31.

Tidball JG

O’Halloran T

Burridge K.

Talin at myotendinous junctions. J Cell Biol. 1986;103:1465-1472.

32.

Tidball JG

Salem G

Zernicke R.

Site and mechanical conditions for failure of skeletal muscle in experimental strain injuries. J Appl Physiol. 1993;74:1280-1286.

33.

Warren GL

Hayes DA

Lowe DA

et al.

Eccentric contraction-induced injury in normal and hindlimb-suspended mouse soleus and EDL muscles. J Appl Physiol. 1994;77:1421-1430.

34.

Weishaupt D

Schweitzer ME

Morrison WB.

Injuries to the distal gastrocnemius muscle: MR findings. J Comput Assist Tomogr. 2001;25:677-682.

35.

Zarins B

Ciullo JV.

Acute muscle and tendon injuries in athletes. Clin Sports Med. 1983;2:167-182.